SIST EN ISO 11732:2005
(Main)Water quality - Determination of ammonium nitrogen - Method by flow analysis (CFA and FIA) and spectrometric detection (ISO 11732:2005)
Water quality - Determination of ammonium nitrogen - Method by flow analysis (CFA and FIA) and spectrometric detection (ISO 11732:2005)
ISO 11732:2005 specifies methods suitable for the determination of ammonium nitrogen in various types of waters (such as ground, drinking, surface, and waste waters) in mass concentrations ranging from 0,1 mg/l to 10 mg/l (in the undiluted sample), applying either FIA or CFA. In particular cases, the range of application may be adapted by varying the operating conditions.
Wasserbeschaffenheit - Bestimmung von Ammoniumstickstoff - Verfahren mittels Fließanalytik (CFA und FIA) und spektrometrischer Detektion (ISO 11732:2005)
Diese Internationale Norm legt Verfahren zur Bestimmung von Ammonium-Stickstoff in unterschiedlichen Wässern fest (wie z. B. Grund-, Trink-, Oberflächen- und Abwässern) in Massenkonzentrationen von 0,1 mg/l bis 10 mg/l (in der unverdünnten Probe), wobei entweder die FIA (Abschnitt 3) oder die CFA (Abschnitt 4) eingesetzt wird. In Einzelfällen kann durch Variation der Arbeitsbedingungen der Anwendungsbereich verändert werden.
Qualité de l'eau - Dosage de l'azote ammoniacal - Méthode par analyse en flux (CFA et FIA) et détection spectrométrique (ISO 11732:2005)
L'ISO 11732:2005 spécifie des méthodes appropriées de dosage de l'azote ammoniacal dans différents types d'eaux (comme l'eau souterraine, l'eau potable, l'eau de surface et l'eau résiduaire), dont les concentrations en masse se situent dans une plage de 0,1 mg/l à 10 mg/l (dans l'échantillon non dilué), en appliquant la FIA (voir Article 3) ou la CFA (voir Article 4). Dans des cas particuliers, la gamme d'application peut être adaptée en faisant varier les conditions opératoires.
Kakovost vode - Določevanje amonijevega dušika – Metoda s pretočno analizo (CFA in FIA) in spektrometrijsko detekcijo (ISO 11732:2005)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2005
1DGRPHãþD
SIST EN ISO 11732:1999
.DNRYRVWYRGH'RORþHYDQMHDPRQLMHYHJDGXãLND±0HWRGDVSUHWRþQRDQDOL]R
&)$LQ),$ LQVSHNWURPHWULMVNRGHWHNFLMR ,62
Water quality - Determination of ammonium nitrogen - Method by flow analysis (CFA and
FIA) and spectrometric detection (ISO 11732:2005)
Wasserbeschaffenheit - Bestimmung von Ammoniumstickstoff - Verfahren mittels
Fließanalytik (CFA und FIA) und spektrometrischer Detektion (ISO 11732:2005)
Qualité de l'eau - Dosage de l'azote ammoniacal - Méthode par analyse en flux (CFA et
FIA) et détection spectrométrique (ISO 11732:2005)
Ta slovenski standard je istoveten z: EN ISO 11732:2005
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11732
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2005
ICS 13.060.50 Supersedes EN ISO 11732:1997
English version
Water quality - Determination of ammonium nitrogen - Method
by flow analysis (CFA and FIA) and spectrometric detection
(ISO 11732:2005)
Qualité de l'eau - Dosage de l'azote ammoniacal - Méthode Wasserbeschaffenheit - Bestimmung von
par analyse en flux (CFA et FIA) et détection Ammoniumstickstoff - Verfahren mittels Fließanalytik (CFA
spectrométrique (ISO 11732:2005) und FIA) und spektrometrischer Detektion (ISO
11732:2005)
This European Standard was approved by CEN on 3 January 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11732:2005: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 11732:2005) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by August 2005, and conflicting national
standards shall be withdrawn at the latest by August 2005.
This document supersedes EN ISO 11732:1997.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 11732:2005 has been approved by CEN as EN ISO 11732:2005 without any
modifications.
INTERNATIONAL ISO
STANDARD 11732
Second edition
2005-02-01
Water quality — Determination of
ammonium nitrogen — Method by flow
analysis (CFA and FIA) and spectrometric
detection
Qualité de l'eau — Dosage de l'azote ammoniacal — Méthode par
analyse en flux (CFA et FIA) et détection spectrométrique
Reference number
ISO 11732:2005(E)
©
ISO 2005
ISO 11732:2005(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2005
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2005 – All rights reserved
ISO 11732:2005(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references . 1
3 Determination of ammonium nitrogen by flow injection analysis (FIA) and spectrometric
detection . 1
4 Determination of ammonium nitrogen by continuous flow analysis (CFA) and
spectrometric detection . 7
5 Calculation. 10
6 Precision . 10
7 Test report . 11
Annex A (informative) Examples of flow analysis systems . 12
Annex B (informative) Precision data. 16
Bibliography . 18
ISO 11732:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11732 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,
chemical and biochemical methods.
This second edition cancels and replaces the first edition (ISO 11732:1997), which has been technically
revised.
iv © ISO 2005 – All rights reserved
ISO 11732:2005(E)
Introduction
Methods using flow analysis are automating wet chemical procedures and are therefore particularly suitable
for the processing of large sample series at a high analysis frequency (up to 100 samples per hour).
[1],[2] [3]
It is differentiated between flow injection analysis (FIA) and continuous flow analysis (CFA) . Both methods
consist of the automatic dosage of the sample introduced into a flow system (manifold) in which the sample
analytes react with the reagent solutions on their way through the manifold. The sample preparation may be
integrated into the manifold. The reaction product is measured in a flow detector.
The user should be aware that particular problems could require the specification of additional marginal
conditions.
INTERNATIONAL STANDARD ISO 11732:2005(E)
Water quality — Determination of ammonium nitrogen —
Method by flow analysis (CFA and FIA) and spectrometric
detection
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this standard be carried
out by suitably trained staff.
1 Scope
This International Standard specifies methods suitable for the determination of ammonium nitrogen in various
types of waters (such as ground, drinking, surface, and waste waters) in mass concentrations ranging from
0,1 mg/l to 10 mg/l (in the undiluted sample), applying either FIA (Clause 3) or CFA (Clause 4). In particular
cases, the range of application may be adapted by varying the operating conditions.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 5667-1, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes
ISO 5667-2, Water quality — Sampling — Part 2: Guidance on sampling techniques
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of water
samples
ISO 8466-1, Water quality — Calibration and evaluation of analytical methods and estimation of performance
characteristics — Part 1: Statistical evaluation of the linear calibration function
3 Determination of ammonium nitrogen by flow injection analysis (FIA) and
spectrometric detection
3.1 Principle
The sample containing ammonium is injected into a continuous carrier stream by means of an injection valve
and is mixed with a continuously streaming flow of an alkaline solution. The ammonia formed is separated in a
diffusion cell from the solution over a hydrophobic semipermeable membrane and taken up by a streaming
recipient flow containing a pH indicator. Due to the resulting pH shift, the indicator solution will change its
colour which is measured continuously in the flow photometer. Additional information about this analytical
technique is given in references [4], [5], [6], [7] and [8].
NOTE Equipment following this principle using CFA instead of FIA is commercially available, however it has not been
validated.
ISO 11732:2005(E)
3.2 Interferences
Volatile amines, if present, diffuse through the membrane and lead to a pH shift. If the concentrations of the
volatile amines (e.g. methylamine or ethylamine) are equal to those of the ammonium, higher results may then
[12]
be expected . Significant concentrations of volatile amines can be reduced by distilling the sample adjusted
to pH 5,8 prior to the analysis.
In rare cases, it may be possible that the sample does not reach a pH of 12 after the addition of the alkaline
reagent solution, thus leading to a loss of ammonium because it will not be converted quantitatively into
ammonia. This may particularly occur with strong acidic or buffered samples. In these cases, the pH of the
sample shall be adjusted to the range of 3 to 5 by the addition of sodium hydroxide solution (3.3.2 or 3.3.3).
A high co
...
SLOVENSKI STANDARD
01-julij-2005
Nadomešča:
SIST EN ISO 11732:1999
Kakovost vode - Določevanje amonijevega dušika – Metoda s pretočno analizo
(CFA in FIA) in spektrometrijsko detekcijo (ISO 11732:2005)
Water quality - Determination of ammonium nitrogen - Method by flow analysis (CFA and
FIA) and spectrometric detection (ISO 11732:2005)
Wasserbeschaffenheit - Bestimmung von Ammoniumstickstoff - Verfahren mittels
Fließanalytik (CFA und FIA) und spektrometrischer Detektion (ISO 11732:2005)
Qualité de l'eau - Dosage de l'azote ammoniacal - Méthode par analyse en flux (CFA et
FIA) et détection spectrométrique (ISO 11732:2005)
Ta slovenski standard je istoveten z: EN ISO 11732:2005
ICS:
13.060.50 Preiskava vode na kemične Examination of water for
snovi chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11732
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2005
ICS 13.060.50 Supersedes EN ISO 11732:1997
English version
Water quality - Determination of ammonium nitrogen - Method
by flow analysis (CFA and FIA) and spectrometric detection
(ISO 11732:2005)
Qualité de l'eau - Dosage de l'azote ammoniacal - Méthode Wasserbeschaffenheit - Bestimmung von
par analyse en flux (CFA et FIA) et détection Ammoniumstickstoff - Verfahren mittels Fließanalytik (CFA
spectrométrique (ISO 11732:2005) und FIA) und spektrometrischer Detektion (ISO
11732:2005)
This European Standard was approved by CEN on 3 January 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11732:2005: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 11732:2005) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by August 2005, and conflicting national
standards shall be withdrawn at the latest by August 2005.
This document supersedes EN ISO 11732:1997.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 11732:2005 has been approved by CEN as EN ISO 11732:2005 without any
modifications.
INTERNATIONAL ISO
STANDARD 11732
Second edition
2005-02-01
Water quality — Determination of
ammonium nitrogen — Method by flow
analysis (CFA and FIA) and spectrometric
detection
Qualité de l'eau — Dosage de l'azote ammoniacal — Méthode par
analyse en flux (CFA et FIA) et détection spectrométrique
Reference number
ISO 11732:2005(E)
©
ISO 2005
ISO 11732:2005(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2005
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2005 – All rights reserved
ISO 11732:2005(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references . 1
3 Determination of ammonium nitrogen by flow injection analysis (FIA) and spectrometric
detection . 1
4 Determination of ammonium nitrogen by continuous flow analysis (CFA) and
spectrometric detection . 7
5 Calculation. 10
6 Precision . 10
7 Test report . 11
Annex A (informative) Examples of flow analysis systems . 12
Annex B (informative) Precision data. 16
Bibliography . 18
ISO 11732:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11732 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,
chemical and biochemical methods.
This second edition cancels and replaces the first edition (ISO 11732:1997), which has been technically
revised.
iv © ISO 2005 – All rights reserved
ISO 11732:2005(E)
Introduction
Methods using flow analysis are automating wet chemical procedures and are therefore particularly suitable
for the processing of large sample series at a high analysis frequency (up to 100 samples per hour).
[1],[2] [3]
It is differentiated between flow injection analysis (FIA) and continuous flow analysis (CFA) . Both methods
consist of the automatic dosage of the sample introduced into a flow system (manifold) in which the sample
analytes react with the reagent solutions on their way through the manifold. The sample preparation may be
integrated into the manifold. The reaction product is measured in a flow detector.
The user should be aware that particular problems could require the specification of additional marginal
conditions.
INTERNATIONAL STANDARD ISO 11732:2005(E)
Water quality — Determination of ammonium nitrogen —
Method by flow analysis (CFA and FIA) and spectrometric
detection
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this standard be carried
out by suitably trained staff.
1 Scope
This International Standard specifies methods suitable for the determination of ammonium nitrogen in various
types of waters (such as ground, drinking, surface, and waste waters) in mass concentrations ranging from
0,1 mg/l to 10 mg/l (in the undiluted sample), applying either FIA (Clause 3) or CFA (Clause 4). In particular
cases, the range of application may be adapted by varying the operating conditions.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 5667-1, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes
ISO 5667-2, Water quality — Sampling — Part 2: Guidance on sampling techniques
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of water
samples
ISO 8466-1, Water quality — Calibration and evaluation of analytical methods and estimation of performance
characteristics — Part 1: Statistical evaluation of the linear calibration function
3 Determination of ammonium nitrogen by flow injection analysis (FIA) and
spectrometric detection
3.1 Principle
The sample containing ammonium is injected into a continuous carrier stream by means of an injection valve
and is mixed with a continuously streaming flow of an alkaline solution. The ammonia formed is separated in a
diffusion cell from the solution over a hydrophobic semipermeable membrane and taken up by a streaming
recipient flow containing a pH indicator. Due to the resulting pH shift, the indicator solution will change its
colour which is measured continuously in the flow photometer. Additional information about this analytical
technique is given in references [4], [5], [6], [7] and [8].
NOTE Equipment following this principle using CFA instead of FIA is commercially available, however it has not been
validated.
ISO 11732:2005(E)
3.2 Interferences
Volatile amines, if present, diffuse through the membrane and lead to a pH shift. If the concentrations of the
volatile amines (e.g. methylamine or ethylamine) are equal to those of the ammonium, higher results may then
[12]
be expected . Significant concentrations of volatile amines can be reduced by distilling the sample adjusted
to pH 5,8 prior to the analysis.
In rare cases, it may be possible that the sample does not reach a pH of 12 after the addition of the alkaline
reagent solution, thus leading to a loss of ammonium because it will not be converted quantitatively into
ammonia. This may particularly occur with strong acidic or buffered samples. In these cases, the pH of the
sample shall be adjusted to the range of 3 to 5 by the addition of sodium hydroxide solution (3.3.2 or 3.3.3).
A high concentration of metal
...
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