SIST EN ISO 19574:2022
(Main)Footwear and footwear components - Qualitative test method to assess antifungal activity (growth test) (ISO 19574:2022)
Footwear and footwear components - Qualitative test method to assess antifungal activity (growth test) (ISO 19574:2022)
This International Standard specifies a test method (growth test) for qualitative evaluation of the antifungal activity of footwear and footwear components due to the action of micro-fungi.
This International Standard is applicable only to footwear and components that claim to have antifungal (antimycotic) or antimicrobial treatment effects.
Schuhe und Schuhbestandteile - Qualitatives Prüfverfahren zur Bestimmung der antimykotischen Wirksamkeit (Wachstumsprüfung) (ISO 19574:2022)
Dieses Dokument legt ein Prüfverfahren (Bewuchstest) zur qualitativen Bewertung der antimykotischen Wirksamkeit von Schuhen und Schuhbestandteilen fest, die der Aktivität von filamentösen Mikropilzen ausgesetzt sind.
Dieses Dokument ist ausschließlich für Schuhe und Schuhbestandteile, für die eine antimykotische (antifungale) oder antimikrobielle Wirkungsweise ausgelobt wird, anzuwenden.
ACHTUNG — Die hier festgelegten Prüfverfahren erfordern die Verwendung von Mikropilzen. Diese Prüfungen dürfen ausschließlich in Einrichtungen mit Eindämmungstechniken für den Umgang mit Mikroorganismen und durch geschultes und mit mikrobiologischen Verfahren vertrautes Personal durchgeführt werden.
Chaussures et composants de chaussures - Méthode d’essai qualitative pour évaluer l'activité antifongique (essai de croissance) (ISO 19574:2022)
Le présent document spécifie une méthode d'essai (essai de croissance) en vue de l'évaluation qualitative de l'activité antifongique de chaussures et de composants de chaussures soumis à l'action de microchampignons filamenteux.
Le présent document ne s'applique qu'aux chaussures et composants de chaussures pour lesquels des effets thérapeutiques antifongiques (antimycotiques) ou antimicrobiens sont déclarés.
Obutev in sestavni deli obutve - Kakovostna preskusna metoda za oceno protiglivične aktivnosti (preskus rasti) (ISO 19574:2022)
Ta mednarodni standard določa preskusno metodo (preskus rasti) za kakovostno ovrednotenje protiglivične aktivnosti obutve in sestavnih delov obutve zaradi delovanja mikrogliv.
Ta mednarodni standard se uporablja samo za obutev in sestavne dele obutve, za katere se trdi, da imajo protiglivične (antimikotične) ali antimikrobne terapevtske učinke.
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 19574:2022
01-april-2022
Obutev in sestavni deli obutve - Kakovostna preskusna metoda za oceno
protiglivične aktivnosti (preskus rasti) (ISO 19574:2022)
Footwear and footwear components - Qualitative test method to assess antifungal
activity (growth test) (ISO 19574:2022)
Schuhe und Schuhbestandteile - Qualitatives Prüfverfahren zur Bestimmung der
antimykotischen Wirksamkeit (Wachstumsprüfung) (ISO 19574:2022)
Chaussures et composants de chaussures - Méthode d’essai qualitative pour évaluer
l'activité antifongique (essai de croissance) (ISO 19574:2022)
Ta slovenski standard je istoveten z: EN ISO 19574:2022
ICS:
61.060 Obuvala Footwear
SIST EN ISO 19574:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 19574:2022
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SIST EN ISO 19574:2022
EN ISO 19574
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2022
EUROPÄISCHE NORM
ICS 61.060
English Version
Footwear and footwear components - Qualitative test
method to assess antifungal activity (growth test) (ISO
19574:2022)
Chaussures et composants de chaussures - Méthode Schuhe und Schuhbestandteile - Qualitatives
d'essai qualitative pour évaluer l'activité antifongique Prüfverfahren zur Bestimmung der antimykotischen
(essai de croissance) (ISO 19574:2022) Wirksamkeit (Wachstumsprüfung) (ISO 19574:2022)
This European Standard was approved by CEN on 18 January 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 19574:2022 E
worldwide for CEN national Members.
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SIST EN ISO 19574:2022
EN ISO 19574:2022 (E)
Contents Page
European foreword . 3
2
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SIST EN ISO 19574:2022
EN ISO 19574:2022 (E)
European foreword
This document (EN ISO 19574:2022) has been prepared by Technical Committee ISO/TC 216
"Footwear" in collaboration with Technical Committee CEN/TC 309 “Footwear” the secretariat of which
is held by UNE.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2022, and conflicting national standards shall
be withdrawn at the latest by August 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 19574:2022 has been approved by CEN as EN ISO 19574:2022 without any modification.
3
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SIST EN ISO 19574:2022
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SIST EN ISO 19574:2022
INTERNATIONAL ISO
STANDARD 19574
First edition
2022-01
Footwear and footwear components —
Qualitative test method to assess
antifungal activity (growth test)
Chaussures et composants de chaussures — Méthode d’essai
qualitative pour évaluer l'activité antifongique (essai de croissance)
Reference number
ISO 19574:2022(E)
© ISO 2022
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SIST EN ISO 19574:2022
ISO 19574:2022(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
© ISO 2022 – All rights reserved
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SIST EN ISO 19574:2022
ISO 19574:2022(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Safety . 2
6 Apparatus . 2
7 Reagents and culture medium .3
7.1 General . 3
7.2 Water . 3
7.3 Malt Extract Agar (MEA) medium. 3
7.3.1 Composition . 3
7.3.2 Preparation . 3
7.4 Physiological saline (sodium chloride solution) . 4
7.4.1 Composition . 4
7.4.2 Preparation . 4
7.5 Wetting agent (nonionic surfactant) . 4
7.6 Buffer solution . 4
7.6.1 Buffer stock . 4
7.6.2 Preparation of buffer stock . 4
7.6.3 Preparation of buffer solution . 4
8 Test microorganisms . 4
9 Preparation of test spore suspension. 5
10 Preparation of test specimens . 6
10.1 General . 6
10.2 Test and control specimens . 6
10.3 Pre-treatment of test and control specimens . 6
11 Test procedure .7
11.1 Inoculation of solidified agar plates . 7
11.2 Arrangement of test and control specimens . 7
11.3 Inoculation of test and control specimens . 7
11.4 Incubation . 7
11.5 Viability control of the spores . . 7
11.6 Assessment of micro-fungal growth . 7
12 Expression of results .8
13 Test report . 8
Bibliography .10
iii
© ISO 2022 – All rights reserved
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SIST EN ISO 19574:2022
ISO 19574:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 216, Footwear, in collaboration with
the European Committee for Standardization (CEN) Technical Committee CEN/TC 309, Footwear, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
© ISO 2022 – All rights reserved
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SIST EN ISO 19574:2022
INTERNATIONAL STANDARD ISO 19574:2022(E)
Footwear and footwear components — Qualitative test
method to assess antifungal activity (growth test)
CAUTION — Test methods specified herein require the use of micro-fungi. These tests shall only
be carried out in facilities with containment techniques for handling microorganisms and by
persons with training and experience in the use of microbiological techniques.
1 Scope
This document specifies a test method (growth test) for the qualitative evaluation of the antifungal
activity of footwear and footwear components exposed to the action of filamentous micro-fungi.
This document is only applicable to footwear and components that claim to have antifungal (antimycotic)
or antimicrobial treatment effects.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for
microbiological examinations
ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media
ISO 16187, Footwear and footwear components — Test method to assess antibacterial activity
ISO 19952, Footwear — Vocabulary
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 19952 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
antifungal activity
antimycotic activity
efficacy of a material or finish used to prevent or mitigate the growth of micro-fungi, to reduce the
number of micro-fungi or to kill micro-fungi
3.2
control specimen
material identical to the test material but without antifungal treatment
1
© ISO 2022 – All rights reserved
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SIST EN ISO 19574:2022
ISO 19574:2022(E)
4 Principle
The test and control specimens of footwear are inoculated with a mixed spore suspension of selected
mould test strains or with a single test strain according to the specific claim.
Antifungal performance is qualitatively determined by visual assessment of fungal growth after
specified or agreed incubation period.
5 Safety
Handling of microorganisms that are potentially hazardous requires a high degree of technical
competence and can be subject to current national legislation and regulations. Only personnel trained
in microbiological techniques should carry out such tests.
NOTE Refer to country-specific codes of practice for personal hygiene, disinfection and sterilizatio
...
SLOVENSKI STANDARD
oSIST prEN ISO 19574:2020
01-marec-2020
Obutev in sestavni deli obutve - Kakovostna preskusna metoda za oceno
protiglivične aktivnosti (preskus rasti) (ISO/DIS 19574:2020)
Footwear and footwear components - Qualitative test method to assess antifungal
activity (growth test) (ISO/DIS 19574:2020)
Schuhe und Schuhbestandteile - Qualitatives Prüfverfahren zur Bestimmung der
antimykotischen Wirksamkeit (Wachstumsprüfung) (ISO/DIS 19574:2020)
Chaussures et composants de chaussures - Méthode d’essai de croissance qualitative
pour évaluer les propriétés antifongiques contre les microchampignons filamenteux
(ISO/DIS 19574:2020)
Ta slovenski standard je istoveten z: prEN ISO 19574
ICS:
61.060 Obuvala Footwear
oSIST prEN ISO 19574:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
oSIST prEN ISO 19574:2020
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oSIST prEN ISO 19574:2020
DRAFT INTERNATIONAL STANDARD
ISO/DIS 19574
ISO/TC 216 Secretariat: UNE
Voting begins on: Voting terminates on:
2020-01-10 2020-04-03
Footwear and footwear components — Qualitative test
method to assess antifungal activity (growth test)
Chaussures et composants de chaussures — Méthode d’essai de croissance qualitative pour évaluer les
propriétés antifongiques contre les microchampignons filamenteux
ICS: 61.060
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 19574:2020(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2020
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oSIST prEN ISO 19574:2020
ISO/DIS 19574:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
---------------------- Page: 4 ----------------------
oSIST prEN ISO 19574:2020
ISO/DIS 19574:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Safety . 2
6 Apparatus . 2
7 Reagents and culture medium . 3
7.1 General . 3
7.2 Water . 3
7.3 Malt extract agar (MEA) medium . 3
7.3.1 Composition . 3
7.3.2 Preparation . 3
7.4 Physiological saline (sodium chloride solution) . 4
7.4.1 Composition . 4
7.4.2 Preparation . 4
7.5 Wetting agent (nonionic surfactant) . 4
7.6 Buffer solution . 4
7.6.1 Buffer stock . 4
7.6.2 Preparation of buffer stock . 4
7.6.3 Preparation of buffer solution . 4
8 Test microorganisms . 4
9 Preparation of test spore suspension . 5
10 Preparation of test specimens . 6
10.1 General . 6
10.2 Test and control specimens . 6
10.3 Pre-treatment of test and control specimens . 6
11 Test procedure . 6
11.1 Inoculation of solidified agar plates . 6
11.2 Arrangement of test and control specimens . 7
11.3 Inoculation of test and control specimens . 7
11.4 Incubation . 7
11.5 Viability control of the spores . 7
11.6 Assessment of micro-fungal growth . 7
12 Expression of results . 7
13 Test report . 8
Bibliography . 9
© ISO 2020 – All rights reserved iii
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oSIST prEN ISO 19574:2020
ISO/DIS 19574:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 216, Footwear.
iv © ISO 2020 – All rights reserved
---------------------- Page: 6 ----------------------
oSIST prEN ISO 19574:2020
DRAFT INTERNATIONAL STANDARD ISO/DIS 19574:2020(E)
Footwear and footwear components — Qualitative test
method to assess antifungal activity (growth test)
CAUTION — Test methods specified herein require the use of micro-fungi. These tests are only
to be carried out in facilities with containment techniques for handling microorganisms and by
persons with training and experience in the use of microbiological techniques.
1 Scope
This International Standard specifies a test method (growth test) for qualitative evaluation of the
antifungal activity of footwear and footwear components due to the action of filamentous micro-fungi.
This International Standard is applicable only to footwear and components that claim to have antifungal
(antimycotic) or antimicrobial treatment effects.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for
microbiological examinations
ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media
ISO 16187, Footwear and footwear components — Test method to assess antibacterial activity
ISO 19952, Footwear — Vocabulary
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 19952 and the following apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at http:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
antifungal activity
antimycotic activity
efficacy of a material or finish used to prevent or mitigate the growth of micro-fungi, to reduce the
number of micro-fungi or to kill micro-fungi
3.2
control specimens
material identical to the test material but without antifungal treatment
Note 1 to entry: If no control specimens are available, sterilized filter paper can be used as control specimens.
© ISO 2020 – All rights reserved 1
---------------------- Page: 7 ----------------------
oSIST prEN ISO 19574:2020
ISO/DIS 19574:2020(E)
4 Principle
The test and control specimens of footwear are inoculated with a mixed spore suspension of selected
mould test strains or with a single test strain according to the specific claim.
Antifungal performance is qualitatively determined by visual assessment of fungal growth after
specified or agreed incubation period.
5 Safety
Handling of microorganisms which are potentially hazardous requires a high degree of technical
competence and can be subject to current national legislation and regulations. Only personnel trained
in microbiological techniques should carry out such tests.
NOTE Refer to country-specific codes of practice for personal hygiene, disinfection and sterilization.
It is recommended that workers should consult IEC 60068-2-10, appendix A “Danger to personnel” and
ISO 7218 “Microbiology — General guidance for microbiological examinations”.
6 Apparatus
6.1 General
Disposable apparatus is an acceptable alternative to re-usable glassware and plastic if it has suitable
specifications.
Usual microbiological laboratory equipment in accordance with ISO 7218 and in particular the
following.
6.2 Biological safety cabinet
6.3 Microbiological incubator
Capable of maintaining a temperature of (28 ± 2) °C and a relative humidity of (85 ± 5) %.
6.4 Autoclave
Capable of maintaining a temperature of (121 ± 2) °C and a pressure of (103 ± 5) kPa, for wet sterilization,
used in accordance with ISO 7218.
6.5 Vortex mixer
6.6 pH-meter, having an accuracy of ± 0,1 pH-units.
1)
6.7 Laboratory centrifugal, 2 000 × g .
6.8 Microscope, magnification × 50.
6.9 Glass beads, 2 mm to 3 mm in diameter, for preparation of fungal spore solutions.
6.10 Glass wool or medical gauze (double layers), for preparation of fungal spore solutions.
6.11 Wide mouth jars, with cap, 500 ml, capable of being used with an autoclave (6.4).
1
...
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