CEN/TC 230/WG 3 - Water analysis - Microbiological methods

This European Standard describes a method for the detection of those serotypes of enterovirus that replicate and form plaques in BGM cells under agar. These will largely be poliovirus and Coxsackievirus B serotypes. Most serotypes of echovirus, Coxsackievirus A and animal enteroviruses are unlikely to produce plaques under these conditions. Reoviruses are unlikely to be detectable within the specified seven day incubation period. It is applicable to any type of water and processed water sample although toxic elements may interfere with cell culture.
The methods to confirm that the plaques seen contain enterovirus are given. Methods to identify which serotype of enterovirus is found in the plaque are not included as this is not required by the EU Bathing Water Directive.
Basic cell culture procedures including the preparation, maintenance and enumeration of BGM cell culture are not described but suggestions are made for media that may be used (see Annex A).

Migrated from Progress Sheet (TC Comment) (2000-07-10): TC 230 Res. 87: // approval procedure on the revision of ISO 7899-2:1984. ++ N315: New TD (TA/980915)

This European Standard specifies a method for the enumeration of culturable micro-organisms in water by counting the colonies formed in a nutrient agar culture medium after aerobic incubation at 36 °C and 22 °C. The method is intended to measure the operational efficiency of the treatment process of public drinking water supplies and for general application to all types of water. It is particulary applicable to the examination of water intended for  human consumption, including water in closed containers and to natural mineral waters.

The method is intended for a concentration range to meet the demands of the EC Drinking Water Directive.

TC - Modifications in Scope and Subclauses 3.1, 8.3 and B.5.3

Migrated from Progress Sheet (TC Comment) (2000-07-10): PQ launched on 1991.05 has failed. A new draft shall be elaborated by CEN/TC 230

ISO 17994:2004 defines an evaluation procedure for comparing two methods intended for the detection or quantification of the same target group or species of microorganisms.
ISO 17994:2004 provides the mathematical basis for the evaluation of the average relative performance of two methods against chosen criteria of equivalence.
Any two enumeration methods based on counts (of colonies or positive tubes) or any two detection methods (presence/absence methods) intended for the same purpose can be compared.
ISO 17994:2004 provides no solution to directly compare a quantitative method (colony count or MPN) with a detection method (P/A).

This European Standard presents a method for the isolation and enumeration of Pseudomonas aeruginosa in bottled water samples by a membrane filtration technique. This method can also be applied to other types of water with a low background flora, for example pool waters and waters intended for human consumption.