This document specifies a laboratory test method for screening anti-fouling paints in a flow-through system using mussels as the test organism. It is intended to be used in conjunction with ISO 21716-1, which specifies the general requirements. The purpose of the test is to determine if there is a difference in mussel settlement on painted test panels compared with mussel settlement on inert non-toxic control panels under the conditions of the test. Examples of statistical analysis to determine if the difference in mussel settlement is statistically significant are given in Annex A.

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This document specifies general requirements and common specifications for preparing and aging panels coated with anti-fouling paint to perform laboratory bioassay screening tests against specified organisms. Such tests are given in the other parts of the ISO 21716 series, with which this document is intended to be used. This document is applicable to all anti-fouling paints that prevent or deter the attachment and growth of sessile organisms on a surface through chemical or biological means. It is not applicable to the following: — coatings that deter or prevent fouling solely by physical means such as biocide-free foul release paints; — anti-fouling methods used for controlling harmful marine organisms and pathogenic organisms in ships' ballast water and sediments according to IMO International Convention for the Control and Management of Ships' Ballast Water and Sediments, 2004[7].

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This document specifies a laboratory test method for screening anti-fouling paints in a flow-through system using barnacle cyprid larvae as the test organism. It is intended to be used in conjunction with ISO 21716-1, which specifies the general requirements. The purpose of the test is to determine if there is a difference in barnacle settlement on painted test panels compared with barnacle settlement on inert non-toxic control panels under the conditions of the test. Examples of statistical analysis to determine if the difference in barnacle settlement is statistically significant are given in Annex A.

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