ISO/TC 126/WG 10 - Intense smoking regime

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of cigarette mainstream smoke with the intense smoking regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC-MS) with methanol as extraction solvent. This method is specified using ISO 20778 smoking parameters. NOTE An alternative method for the determination of B[a]P is specified in ISO 23906-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry (GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones by reversed phase HPLC-UV/DAD in mainstream smoke using an intense smoking regime.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 20778 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 20 mg/cigarette and 45 mg/cigarette.

This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 3308 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 1 mg/cigarette and 16 mg/cigarette.

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 20778 smoking parameters. This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

This document specifies a method for determination of carbon monoxide (CO) in the vapour phase of cigarette smoke with an intense smoking regime.

This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke with an intense smoking regime.

This document describes the requirements for a monitor test piece as well as its use.

This document: — defines smoking parameters and specifies the conditions to be provided for the routine analytical machine smoking of cigarettes under specified intense conditions; — specifies the requirements for a routine analytical smoking machine conforming with these conditions; — specifies additional test methods for machine verification. Annex A gives examples of designs of smoking machine enclosures. NOTE The type of analytical smoking machine used could impact the mass of total particulate matter collected on the smoke trap. This might be due to either incomplete collection or evaporation of water and other volatile compounds from the smoke trap.

This document specifies a method for the generation and collection of total particulate matter (TPM) present in the smoke from cigarettes using a routine analytical smoking machine with an intense smoking regime. It is not applicable to the determination of water or nicotine-free dry particulate matter when using the extraction procedure specified in ISO 4387:2000, 7.9.1 or the subsequent measurement of water specified in ISO 10362-1 or ISO 10362-2. NOTE The type of analytical smoking machine used could impact the mass of TPM collected on the smoke trap. This might be due to either incomplete collection or evaporation of water and other volatile compounds from the smoke trap.

ISO/TR 19478-2:2015 extends the analysis reported in ISO/TR 19478‑1:2014 and reports additional studies focused on the conclusions i) and j) from that Technical Report. It identifies and assesses factors impacting on the measurement of smoke TPM, NFDPM, nicotine, water, and carbon monoxide yields when increasing the intensity of the puffing regime from that specified in ISO 3308:2000 to the regime specified in Health Canada Method T-115.

ISO/TR 19478-1:2014 comprises a description of the collaborative study undertaken by WG 10 during 2010 and 2011 and an analysis of the data generated in the study.