ISO 23923:2020
(Main)Cigarettes — Determination of selected volatile organic compounds in the mainstream smoke of cigarettes with an intense smoking regime — Method using GC/MS
Cigarettes — Determination of selected volatile organic compounds in the mainstream smoke of cigarettes with an intense smoking regime — Method using GC/MS
This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry (GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.
Cigarettes — Dosage de composés organiques volatils sélectionnés dans le courant principal de la fumée de cigarette avec un régime de fumage intense — Méthode par CG-SM
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INTERNATIONAL ISO
STANDARD 23923
First edition
2020-09
Cigarettes — Determination of
selected volatile organic compounds
in the mainstream smoke of cigarettes
with an intense smoking regime —
Method using GC/MS
Cigarettes — Dosage de composés organiques volatils sélectionnés
dans le courant principal de la fumée de cigarette avec un régime de
fumage intense — Méthode par CG-SM
Reference number
©
ISO 2020
© ISO 2020
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ii © ISO 2020 – All rights reserved
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 1
6 Reagents . 2
7 Preparation . 2
7.1 Preparation of glassware. 2
7.2 Preparation of standards. 3
7.2.1 General. 3
7.2.2 Preparation of internal standard spiking solution . 3
7.2.3 Preparation of working standards for isoprene, acrylonitrile, benzene,
and toluene . 3
7.2.4 Preparation of working standards for 1,3-butadiene . 4
8 Sampling . 5
9 Tobacco product preparation . 5
10 Sample generation — Smoking of cigarettes . 5
10.1 General . 5
10.2 Smoking machine setup . 5
10.3 Smoking . 7
10.3.1 General. 7
10.3.2 Linear smoking . 7
10.3.3 Rotary smoking . 7
11 Sample analysis . 7
11.1 Preparation of sample. 7
11.2 Determination . 8
11.2.1 GC-MS operating conditions . 8
11.2.2 Calibration . 9
11.2.3 Calculation . 9
12 Repeatability and reproducibility . 9
12.1 General . 9
12.2 Results of the 2012 collaborative study .10
13 Test report .12
Annex A (informative) Examples of chromatograms .13
Bibliography .17
Foreword
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electrotechnical standardization.
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described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
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This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
Any feedback or questions on this document should be directed to the user’s national standards body. A
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iv © ISO 2020 – All rights reserved
Introduction
The CORESTA (www .coresta .org) Special Analytes Sub-Group (since 2017 the Sub-Group changed
its name to Smoke Analytes Sub-Group) carried out a collaborative study in 2005 to compare smoke
analyte yields of selected volatile organic compounds (volatiles) obtained from different laboratories
using their own preferred methodologies. This study reported significant and unacceptable differences
in volatiles yields, especially for 1,3-butadiene and acrylonitrile and suggested that further work was
required to understand factors influencing the variability. Key parameters of existing methodologies
[1] [2]
were reviewed and further studies were conducted on selected volatiles between 2008 and 2009 .
These studies investigated critical method steps that required optimization before incorporation
into a CORESTA Recommended Method (CRM). The CRM was based on collecting the volatiles from
mainstream cigarette smoke in cryogenically cooled impinger traps containing methanol. The impinger
solutions were fortified with benzene-D and analysed by gas chromatography/mass spectrometry
(GC-MS).
This document was produced through a CORESTA collaborative study conducted in 2011, involving 17
[3]-[5]
laboratories from 11 countries and included 10 samples with different tar yields . Cigarettes were
smoked with the intense smoking regime specified in Health Canada Official Method T-115 (equivalent
to ISO 20778). Statistical evaluations carried out according to ISO 5725-1 and ISO 5725-2 are included.
No machine smoking regime can represent all human smoking behaviour.
— It is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document.
— Machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about differences in exposure and risk across brands.
— Smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using International Standards
INTERNATIONAL STANDARD ISO 23923:2020(E)
Cigarettes — Determination of selected volatile organic
compounds in the mainstream smoke of cigarettes with an
intense smoking regime — Method using GC/MS
WARNING — The use of this document can involve hazardous materials, operations and
equipment. This document does not purport to address all the safety problems associated with
its use. It is the responsibility of the user of this document to establish appropriate safety and
health practices and determine the applicability of any other restrictions prior to use.
1 Scope
This document specifies a method for the quantification of selected volatile organic compounds (VOCs:
1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry
(GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 8243, Cigarettes — Sampling
ISO 20778, Cigarettes — Routine analytical cigarette smoking machine — Definitions and standard
conditions with an intense smoking regime
ISO 20779, Cigarettes — Generation and collection of total particulate matter using a routine an
...
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