This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISOÂ 4387.
NOTEÂ Â Â Â Â Â ISOÂ 20778 and ISOÂ 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

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This document specifies a method for the determination of water content by the Karl Fischer method. It is applicable to raw tobacco as well as tobacco taken from finished products. The method is suitable for water contents ranging from a mass fraction of at least 2Â % to 55Â %.

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This document recollects and evaluates physical parameters of filter paper substrates for the determination of ignition propensity of cigarettes according to ISOÂ 12863.

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This document specifies a gas-chromatographic (GC) method for the determination of water content. It is applicable to raw tobacco as well as tobacco taken from finished products. The method is suitable for water contents ranging at least from a mass fraction of 2Â % to 55Â %.

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This document describes the requirements for a monitor test piece for smoking machine as well as its use.

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This document specifies a method for the determination of water content by the Karl Fischer method. It is applicable to raw tobacco as well as tobacco taken from finished products. The method is suitable for water contents ranging from a mass fraction of at least 2Â % to 55Â %.

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This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISOÂ 4387. NOTEÂ Â Â Â Â Â ISOÂ 20778 and ISOÂ 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

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This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in tobacco and the following tobacco products: cigarettes, cigars and smokeless tobacco products using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The TSNAs determined with this method are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

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This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

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This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by gas chromatography/mass spectrometry (GC-MS) in mainstream cigarette smoke using ISO 20778 smoking parameters.

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This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of cigarette mainstream smoke with the intense smoking regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

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This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC-MS) with methanol as extraction solvent. This method is specified using ISO 20778 smoking parameters. NOTE An alternative method for the determination of B[a]P is specified in ISO 23906-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

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This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones by reversed phase HPLC-UV/DAD in mainstream smoke using an intense smoking regime.

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This document specifies reference e-liquids to be used to test emissions generated by electronic cigarettes [1].
This document applies to the reference e-liquids to be used when an electronic cigarette is sold empty, without an e-liquid, and where the product information or instructions for use are not specific in terms of the compositional characteristics of the e-liquid to be used with the device.

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This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 20778 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 20 mg/cigarette and 45 mg/cigarette.

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This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 20778 smoking parameters. This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

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This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 3308 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol, m-Cresol and o-Cresol. This method is applicable to cigarettes with total particulate matter (TPM) yields between 1 mg/cigarette and 16 mg/cigarette.

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This document specifies a method for the determination of the content of total alkaloids as nicotine in tobacco by continuous-flow analysis. This method is applicable to leaf samples, stems, reconstituted tobacco sheet materials and tobacco blends.

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This document specifies additional test methods for routine analytical cigarette-smoking machines intended to check the conformity of these machines with ISO 3308 and ISO 20778.
It only establishes additional test methods for smoking machines and does not deal with actual smoking, which is described in other International Standards.
It is composed of four sections relating to
— the determination of pressure drop (Clause 4);
— the determination of significant puff profile parameters (Clause 5);
— the verification of restricted smoking (Clause 6);  
— the description of the soap film bubble flowmeter for the determination of the puff volume (Clause 7).
NOTE There are more possibilities for determining the aforementioned parameters. Systems giving the same results and accuracies can be used. The certificate of conformity with this standard can be obtained from the machine manufacturer.

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This document specifies a method for the determination of air permeability (AP).
It is applicable to materials used as cigarette papers, filter plug wrap and filter joining paper, including materials having an oriented permeable zone or discrete permeable zones where the measured permeability is in excess of 10 cm3·(min−1·cm−2) at 1 kPa. In addition, it is applicable to banded cigarette papers, with bands of a width of at least 4 mm.
NOTE For an estimate of the air permeability of materials outside the scope of this document, see Note 3 in 5.1.2 and 7.6.1.4.

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This document specifies the general principles to be applied when sampling batches of raw tobacco in order to assess either
— the mean value of one or more of its characteristics, or
— the heterogeneity of one or more of its characteristics.
Manufactured tobacco products, including products intended for sale or distribution, are specifically not included in the scope of this document.
This document is applicable to the sampling of batches of raw tobacco of the following types:
a) leaf tobacco:  
flue cured;
air cured;
sun cured;
fire cured;  
b) pretreated raw tobacco:  
which has undergone fermentation (in packages not intended for retail or wholesale sales or distribution, in bulk, in chambers);
which has been partially or completely stemmed;
which is in the form of stems;
which is in the form of waste and remnants;
which has been reconstituted in the form of strips.

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This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

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This document specifies a method for the gas-chromatographic determination of water in total particulate matter from the mainstream smoke. The smoking of cigarettes and collection of mainstream smoke are normally carried out in accordance with ISO 4387. However, the method of this document is also applicable to the determination of water in total particulate matter from the mainstream smoke obtained by non-standard smoking.
NOTE In countries not in a position to use the gas-chromatographic method, the determination of water in total particulate matter from the mainstream smoke is performed using the Karl Fischer method (see ISO 10362-2). In such cases, values obtained for water in total particulate matter from the mainstream smoke are used with the addition of a note made in the expression of the results.

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This document specifies a method for the determination of ventilation which is applicable to cigarettes.

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This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 3308 smoking parameters. This method is applicable to cigarettes with ammonia yields between 1 µg/cigarette and 30 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

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This document specifies a method for the gravimetric determination of the purity of nicotine using tungstosilicic acid. The method is applicable to pure nicotine or nicotine salts used to calibrate analytical methods for the determination of nicotine in the field of tobacco, tobacco products and smoke analysis.

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This document specifies the general principles to be applied when sampling batches of raw tobacco in order to assess either — the mean value of one or more of its characteristics, or — the heterogeneity of one or more of its characteristics. Manufactured tobacco products, including products intended for sale or distribution, are specifically not included in the scope of this document. This document is applicable to the sampling of batches of raw tobacco of the following types: a) leaf tobacco: flue cured; air cured; sun cured; fire cured; b) pretreated raw tobacco: which has undergone fermentation (in packages not intended for retail or wholesale sales or distribution, in bulk, in chambers); which has been partially or completely stemmed; which is in the form of stems; which is in the form of waste and remnants; which has been reconstituted in the form of strips.

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This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with methanol as extraction solvent. This method is specified using ISO 3308 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 22634-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.

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This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. This method is specified using ISO 3308 smoking parameters. This document provides an alternative method to that specified in ISO 22634-1, with a different clean-up, and a shorter total analytical run allowing a potential increase of sample throughput in comparison with ISO 22634-1.

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This document specifies a method for determination of carbon monoxide (CO) in the vapour phase of cigarette smoke with an intense smoking regime.

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This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

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This document specifies an analytical method to quantify the nicotine, propylene glycol and glycerol content in e-liquids by gas chromatography.

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This document specifies a method for the determination of the width of strands of cut tobacco. This method is only applicable on samples of strands of cut tobacco with a uniform cut width. NOTE There are other ways of measuring the width of the strands of cut tobacco. A system with the same accuracy can be used, for example a microscope with an internal fitted ruler or a camera with image processing system.

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This document specifies a method for the determination of air permeability (AP). It is applicable to materials used as cigarette papers, filter plug wrap and filter joining paper, including materials having an oriented permeable zone or discrete permeable zones where the measured permeability is in excess of 10 cm3·(min−1·cm−2) at 1 kPa. In addition, it is applicable to banded cigarette papers, with bands of a width of at least 4 mm. NOTE For an estimate of the air permeability of materials outside the scope of this document, see Note 3 in 5.1.2 and 7.6.1.4.

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This document specifies a method for the gas-chromatographic determination of water in total particulate matter from the mainstream smoke. The smoking of cigarettes and collection of mainstream smoke are normally carried out in accordance with ISO 4387. However, the method of this document is also applicable to the determination of water in total particulate matter from the mainstream smoke obtained by non-standard smoking. NOTE In countries not in a position to use the gas-chromatographic method, the determination of water in total particulate matter from the mainstream smoke is performed using the Karl Fischer method (see ISO 10362-2). In such cases, values obtained for water in total particulate matter from the mainstream smoke are used with the addition of a note made in the expression of the results.

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This document specifies a method for the determination of ventilation which is applicable to cigarettes.

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This document specifies a method for the determination of carbon monoxide (CO) in the vapour phase of water pipe tobacco smoke.

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This document specifies a method for the determination of carbon monoxide (CO) emission of glowing water pipe charcoal.

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This document specifies methods for the determination of total collected matter and for the subsequent determination of nicotine present in the smoke from water pipe tobacco generated and collected using a water pipe tobacco smoking machine. This document is only applicable for devices known as "arghile", "hookah", "nargile" or "shisha" in which tobacco is only heated, not combusted. Other types, such as "Chinese water pipe", are not covered.

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This document — defines smoking parameters and specifies the standard conditions to be provided for the routine analytical machine smoking of water pipe tobacco, where the water pipe tobacco sample is electrically heated only and not combusted; — specifies the requirements for a routine analytical smoking machine complying with the standard conditions. This document is only applicable for water pipe tobacco used in devices known as "arghile", "hookah", "nargile" or "shisha" in which tobacco is heated, not combusted. Other types of water pipe tobacco that are used in other devices such as "Chinese water pipe" are not covered.

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This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke with an intense smoking regime.

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This document is applicable to electronic cigarettes and similar vapour producing devices intended for the production of aerosol from e-liquids for consumption by inhalation. It is applicable to devices intended for use with or without nicotine content in the aerosol produced.  This standard is also applicable to e-liquid containers, filling mechanisms and accessories, electrical and other, intended for use with electronic cigarettes and similar vapour producing devices.
This standard specifies the minimum safety and technical requirements for electronic cigarette devices, e-liquid containers, and associated accessories when operated and maintained in the manner prescribed by the manufacturer.

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This document is applicable to electronic cigarettes and similar vapour producing devices intended for the production of aerosol from e-liquids for consumption by inhalation. It is applicable to devices intended for use with or without nicotine content in the aerosol produced. This standard is also applicable to e-liquid containers, filling mechanisms and accessories, electrical and other, intended for use with electronic cigarettes and similar vapour producing devices.
This standard specifies the minimum safety and technical requirements for electronic cigarette devices, e-liquid containers, and associated accessories when operated and maintained in the manner prescribed by the manufacturer.

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This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD. This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.

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This document specifies a method for the quantification of selected volatile organic compounds (VOCs: 1,3-butadiene, isoprene, acrylonitrile, benzene and toluene) by GC-MS in mainstream cigarette smoke using ISO 3308 smoking parameters. This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette.

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This European Technical Report gives a list of constituents of interest proposed for measurement in the aerosol for the purpose of regulatory submission under the Directive 2014/40/EU (TPD) [2] , for:
—   prefilled products such as disposable e-cigarettes and refill cartridges;
—   e-liquids sold in refill containers;
—   the following categories of hardware: coils or other heater elements of the vaping product, atomisers, rebuildable atomisers and all open tank or dripper products with inbuilt atomisers, including clearomisers.
This list is not intended to be comprehensive but rather, it represents the default minimum requirement. Depending on the device/liquid combination and the toxicological assessment other substances might have to be measured as well.

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This document specifies a method for the generation and collection of total particulate matter (TPM) present in the smoke from cigarettes using a routine analytical smoking machine with an intense smoking regime. It is not applicable to the determination of water or nicotine-free dry particulate matter when using the extraction procedure specified in ISO 4387:2000, 7.9.1 or the subsequent measurement of water specified in ISO 10362-1 or ISO 10362-2. NOTE The type of analytical smoking machine used could impact the mass of TPM collected on the smoke trap. This might be due to either incomplete collection or evaporation of water and other volatile compounds from the smoke trap.

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