Standard Test Methods for Determination of the Effects of Biogenic Acidification on Concrete Antimicrobial Additives and/or Concrete Products

SIGNIFICANCE AND USE
4.1 As described in Guide C1894, the MIC of concrete is considered to be a three-stage process with the reduction in pH (Stage I) (for example, 12.5 > pH > 9-10), the establishment of biofilms which further lowers the pH (Stage II) (for example, 9-10 > pH > 4-6) and eventual deterioration due to biogenic acid exposure (Stage III) (for example,  
4.2 The tests are performed in simulated exposure solutions containing well-controlled bacterial strains that are grown in the laboratory. These tests do not require an environmental chamber and are intended to be performed as benchtop tests in biosafety level 1 laboratory conditions. These tests are suitable for simulation of the Stage II and III of MIC because the pH range of the solution can be controlled within the ranges of each stage.  
4.3 This standard provides three test methods.  
4.3.1 Test Method A is suitable for assessing the efficacy of antimicrobial admixtures in delaying or preventing biogenic acidification in a nutrient-rich simulated wastewater exposure solution.  
4.3.2 Test Method B is suitable for assessing the effectiveness of antimicrobial admixtures in a prescribed cementitious system (Option B1) or assessing the performance of different cementitious systems (Option B2) in delaying or preventing microbially-induced corrosion of concrete in the Stage II of MIC.  
4.3.3 Test Method C is suitable for assessing the suitability of cementitious systems in delaying or preventing microbially-induced corrosion of concrete in the Stage III of MIC.  
4.4 The results obtained by these test methods should serve as information to be used with Guide C1894 in, but not as the sole basis for, selection of a biologically-resistant material for a particular application. No attempt has been made to incorporate into these test methods all the various factors that may affect the performance of a material when subjected to actual service.
SCOPE
1.1 This standard presents test methods for the determination of the effects of biogenic acidification on concrete products and/or efficacy of antimicrobial products to resist microbially-induced corrosion (MIC) of concrete. In these tests, the biogenic acidification is achieved by sulfur-oxidizing bacteria (SOB) that can convert elemental sulfur or thiosulfate to sulfuric acid without the use of H2S gas.  
1.2 This standard is referenced in the guideline document for MIC of concrete products. Guide C1894 provides guidance for microbially-induced corrosion of concrete products and an overview of where this test, and its options, can and should be used. This document is not intended to be a guideline document for MIC of concrete products.  
1.3 This standard does not cover controlled breeding chamber tests, in which H2S gas is produced by bacterial activity and acidification is the result of the conversion of this H2S gas to sulfuric acid.  
1.4 This standard does not cover chemical acid immersion tests, in which acidification is achieved by chemical sulfuric acid addition, not by bacterial activity. Testing protocols for chemical acid immersion are described in Test Methods C267 and C1898.  
1.5 This standard does not cover tests that assess field exposure conditions or sewage pipe, concrete tank, or concrete riser network design.  
1.6 This standard does not cover live trial tests where concrete coupons or other specimens are monitored in sewers.  
1.7 The tests described in this standard should not be performed on concrete samples that have already been exposed to MIC conditions.  
1.8 This standard does not cover concrete deterioration due to chemical sulfate attack, which is caused by the reaction of sulfate compounds that exist in wastewater with the hydration products of cement. Test methods for assessing sulfate attack are provided by Test Methods C452 and C1012/C1012M.  
1.9 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this ...

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ASTM C1904-20 - Standard Test Methods for Determination of the Effects of Biogenic Acidification on Concrete Antimicrobial Additives and/or Concrete Products
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:C1904 −20
Standard Test Methods for
Determination of the Effects of Biogenic Acidification on
1
Concrete Antimicrobial Additives and/or Concrete Products
This standard is issued under the fixed designation C1904; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope products of cement. Test methods for assessing sulfate attack
are provided by Test Methods C452 and C1012/C1012M.
1.1 This standard presents test methods for the determina-
tion of the effects of biogenic acidification on concrete prod- 1.9 The values stated in SI units are to be regarded as
ucts and/or efficacy of antimicrobial products to resist standard. No other units of measurement are included in this
microbially-induced corrosion (MIC) of concrete. In these standard.
tests, the biogenic acidification is achieved by sulfur-oxidizing
1.10 This standard does not purport to address all of the
bacteria (SOB) that can convert elemental sulfur or thiosulfate
safety concerns, if any, associated with its use. It is the
to sulfuric acid without the use of H S gas.
2 responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter-
1.2 This standard is referenced in the guideline document
mine the applicability of regulatory limitations prior to use.
forMICofconcreteproducts.GuideC1894providesguidance
1.11 This international standard was developed in accor-
for microbially-induced corrosion of concrete products and an
dance with internationally recognized principles on standard-
overview of where this test, and its options, can and should be
ization established in the Decision on Principles for the
used. This document is not intended to be a guideline docu-
Development of International Standards, Guides and Recom-
ment for MIC of concrete products.
mendations issued by the World Trade Organization Technical
1.3 This standard does not cover controlled breeding cham-
Barriers to Trade (TBT) Committee.
bertests,inwhichH Sgasisproducedbybacterialactivityand
2
acidification is the result of the conversion of this H S gas to
2
2. Referenced Documents
sulfuric acid.
2
2.1 ASTM Standards:
1.4 This standard does not cover chemical acid immersion
C125Terminology Relating to Concrete and Concrete Ag-
tests, in which acidification is achieved by chemical sulfuric
gregates
acid addition, not by bacterial activity. Testing protocols for
C150/C150MSpecification for Portland Cement
chemical acid immersion are described in Test Methods C267
C192/C192MPracticeforMakingandCuringConcreteTest
and C1898.
Specimens in the Laboratory
1.5 This standard does not cover tests that assess field C260/C260MSpecification for Air-Entraining Admixtures
exposure conditions or sewage pipe, concrete tank, or concrete
for Concrete
riser network design. C267Test Methods for Chemical Resistance of Mortars,
Grouts,andMonolithicSurfacingsandPolymerConcretes
1.6 This standard does not cover live trial tests where
C452Test Method for Potential Expansion of Portland-
concrete coupons or other specimens are monitored in sewers.
Cement Mortars Exposed to Sulfate
1.7 The tests described in this standard should not be
C494/C494MSpecification for Chemical Admixtures for
performedonconcretesamplesthathavealreadybeenexposed
Concrete
to MIC conditions.
C595/C595MSpecification for Blended Hydraulic Cements
1.8 This standard does not cover concrete deterioration due C618Specification for Coal Fly Ash and Raw or Calcined
to chemical sulfate attack, which is caused by the reaction of Natural Pozzolan for Use in Concrete
sulfate compounds that exist in wastewater with the hydration C822Terminology Relating to Concrete Pipe and Related
Products
1
These test methods are under the jurisdiction of ASTM Committee C13 on
2
Concrete Pipe and is the direct responsibility of Subcommittee C13.03 on For referenced ASTM standards, visit the ASTM website, www.astm.org, or
DeterminingtheEffectsofBiogenicSulfuricAcidonConcretePipeandStructures. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved Oct. 1, 2020. Published October 2020. DOI: 10.1520/ Standards volume information, refer to the standard’s Document Summary page on
C1904_C1904M-20 the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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C1904−20
C989/C989MSpecification for Slag Cement for Use in biosafetylevel1laboratoryconditions.Thesetestsaresuitable
Concrete and Mortars for simulation of the Stage II and III o
...

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