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ASTM E3285-22

(Test Method)

Standard Test Method for Evaluation of Contact-Mediated Microbial Transference

Standard Test Method for Evaluation of Contact-Mediated Microbial Transference

SIGNIFICANCE AND USE
4.1 This test method utilizes filter paper as a medium for evaluating touch-transfer of bacteria to material surfaces. Sample surfaces are exposed to filter papers saturated with a Staphylococcus aureus suspension, followed by recovery of the bacteria from the surface with Replicate Organism Detection and Counting (RODAC) plates. This test method reports the log10 reduction of bacterial transfer on an intended test surface compared to a control surface. The test and control surfaces can differ by texture, coating, treatment, or any other desired variables, as long as they are the same material.
SCOPE
1.1 This test method is designed to evaluate the contact-mediated microbial transference on two-dimensional material surfaces. Material surfaces intended to reduce microorganism adherence or contamination may be evaluated using this test method. Additionally, this test method can be utilized on a wide variety of material surfaces for other downstream processes.  
1.2 This test method is designed to quantify the fomite transfer of bacteria on material surfaces through touch-transfer, be inexpensive, require limited training in practice, and be adaptable to many surfaces or microorganisms. Non-porous surfaces are contaminated with inoculated filter paper as a carrier and surface contamination is quantified by sampling with Replicate Organism Detection and Counting (RODAC) plates.  
1.3 Basic microbiology training is required to perform this test method.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Published
Publication Date
14-Mar-2022
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

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ASTM E3285-22 - Standard Test Method for Evaluation of Contact-Mediated Microbial TransferenceASTM E3285-22 - Standard Test Method for Evaluation of Contact-Mediated Microbial Transference
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ASTM E3285-22 - Standard Test Method for Evaluation of Contact-Mediated Microbial Transference
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3285 − 22
Standard Test Method for
1
Evaluation of Contact-Mediated Microbial Transference
This standard is issued under the fixed designation E3285; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope E1054PracticesforEvaluationofInactivatorsofAntimicro-
bial Agents
1.1 This test method is designed to evaluate the contact-
E2756Terminology Relating toAntimicrobial andAntiviral
mediated microbial transference on two-dimensional material
Agents
surfaces. Material surfaces intended to reduce microorganism
adherence or contamination may be evaluated using this test
3. Terminology
method.Additionally,thistestmethodcanbeutilizedonawide
3.1 Definitions—For definitions of terms used in this test
variety of material surfaces for other downstream processes.
method, refer to Terminology E2756.
1.2 This test method is designed to quantify the fomite
3.2 Definitions of Terms Specific to This Standard:
transferofbacteriaonmaterialsurfacesthroughtouch-transfer,
3.2.1 microbialtransference,n—relocationofamicroorgan-
be inexpensive, require limited training in practice, and be
ism from a previously contaminated surface to a different
adaptable to many surfaces or microorganisms. Non-porous
surface by contact.
surfaces are contaminated with inoculated filter paper as a
carrier and surface contamination is quantified by sampling
4. Significance and Use
with Replicate Organism Detection and Counting (RODAC)
4.1 This test method utilizes filter paper as a medium for
plates.
evaluating touch-transfer of bacteria to material surfaces.
1.3 Basic microbiology training is required to perform this
Sample surfaces are exposed to filter papers saturated with a
test method.
Staphylococcusaureussuspension,followedbyrecoveryofthe
bacteria from the surface with Replicate Organism Detection
1.4 The values stated in SI units are to be regarded as
and Counting (RODAC) plates. This test method reports the
standard. No other units of measurement are included in this
log reduction of bacterial transfer on an intended test surface
10
standard.
comparedtoacontrolsurface.Thetestandcontrolsurfacescan
1.5 This standard does not purport to address all of the
differ by texture, coating, treatment, or any other desired
safety concerns, if any, associated with its use. It is the
variables, as long as they are the same material.
responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter- 5. Apparatus
mine the applicability of regulatory limitations prior to use.
5.1 Calibrated timer—readable in minutes and seconds,
1.6 This international standard was developed in accor-
5.2 Cell spreader—sterile,
dance with internationally recognized principles on standard-
ization established in the Decision on Principles for the 5.3 Centrifuge—capable of 2500 × g,
Development of International Standards, Guides and Recom-
5.4 Forceps—sterile,
mendations issued by the World Trade Organization Technical
5.5 Incubator—capable of maintaining a temperature of
Barriers to Trade (TBT) Committee.
35°C 62°C,
2. Referenced Documents
5.6 Thermometer or thermocouple,
2
2.1 ASTM Standards:
5.7 Variable volume pipettors and sterile tips, capable of
dispensing volumes from 10 µL-1000 µL, with current
1
This test method is under the jurisdiction of ASTM Committee E35 on
calibration,
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
5.8 Vortex mixer—any vortex mixer that will ensure proper
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved March 15, 2022. Published April 2022. DOI: 10.1520/
agitation and mixing of the contents of test tubes.
E3285–22
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or 6. Reagents and Materials
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
6.1 Reagents:
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. 6.1.1 Ethanol—commercially available 95% ethanol.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E3285 − 22
NOTE 2—Additional challenge species may be substituted to evaluate
6.1.2 Phosphate Buffered Saline (PBS)—Prepare according
the breadth of a material’s antimicrobial activity versus species to which
to manufacturer’s instructions t
...

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1.1 This test method covers the enumeration of bacteria that can tolerate high salt concentrations or can hydrolyze protein/collagen, or both. This test method is applicable to raceway brine, brine-cured hides and skins, and pre-charge raceway liquor.  
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