Standard Test Method for Efficacy of Fungal Control Agents as Preservatives for Aqueous-Based Products Used in the Paper Industry

SIGNIFICANCE AND USE
This test method should be used to determine if a fungal control agent is effective to preserve pigment suspensions, dye solutions, pulp slurries, starch solutions, polymers, sizing agents, latex emulsions, and other specific aqueous-based materials used in the paper industry. Separate evaluations should be made on a representative type for each specific class of product to be preserved.
Note 1—Control of bacterial spoilage of similar products can be evaluated by Test Method E723.
Note 2—Slimicides for control of fungal or bacterial slime can be evaluated by Test Method E1839.
SCOPE
1.1 This laboratory test method is used to determine the efficacy of a fungal control agent to prevent spoilage of in-process aqueous-based products used in the paper industry.
1.2 For information on bacterial control agents, see Test Method E723.
1.3 It is the responsibility of the investigator to determine whether good laboratory practices (GLP) are required and to follow them when appropriate (see 40 CFR 160).  
1.4 A knowledge of microbiological techniques is required for these procedures.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E875 − 10
StandardTest Method for
Efficacy of Fungal Control Agents as Preservatives for
1
Aqueous-Based Products Used in the Paper Industry
This standard is issued under the fixed designation E875; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2.2 Federal Standard:
3
40 CFR 160 Good Laboratory Practice Standards
1.1 This laboratory test method is used to determine the
efficacy of a fungal control agent to prevent spoilage of
3. Terminology
in-process aqueous-based products used in the paper industry.
3.1 Definitions of Terms Specific to This Standard:
1.2 For information on bacterial control agents, see Test 3.1.1 fungal control agent, n—an agent that either kills or
Method E723. prevents growth of fungi and either kills or prevents the
germination of fungal spores. This term is applied to chemical
1.3 It is the responsibility of the investigator to determine
biocidal or biostatic agents.
whether good laboratory practices (GLP) are required and to
3.1.2 preservative, n—chemical agent used to prevent mi-
follow them when appropriate (see 40 CFR 160).
crobial soilage of products due to microbial growth.
1.4 A knowledge of microbiological techniques is required
4. Summary of Test Method
for these procedures.
4.1 Aqueous material to be preserved is inoculated with an
1.5 The values stated in SI units are to be regarded as
appropriate fungal inoculum followed by addition of a concen-
standard. No other units of measurement are included in this
tration of fungal control agent that will kill the fungi or prevent
standard.
their growth for a desired period of time, or both. In addition,
1.6 This standard does not purport to address all of the
the agent will also prevent fungal spore germination. Fungal
safety concerns, if any, associated with its use. It is the growth is determined by visible signs of deterioration in the
responsibility of the user of this standard to establish appro- test sample, and by obtaining fungal numbers and comparing
them to a sample without any fungal control agent. The proper
priate safety and health practices and determine the applica-
level of fungal control agent is one that prevents product
bility of regulatory limitations prior to use.
deterioration and reduces and keeps the organisms to an
acceptable level in the test material, as determined by the tester
2. Referenced Documents
or user.
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
5. Significance and Use
E723 Test Method for Efficacy of Antimicrobials as Preser-
5.1 This test method should be used to determine if a fungal
vatives for Aqueous-Based Products Used in the Paper
control agent is effective to preserve pigment suspensions, dye
Industry (Bacterial Spoilage)
solutions, pulp slurries, starch solutions, polymers, sizing
E1839 Test Method for Efficacy of Slimicides for the Paper
agents, latex emulsions, and other specific aqueous-based
Industry—Bacterial and Fungal Slime
materials used in the paper industry. Separate evaluations
should be made on a representative type for each specific class
of product to be preserved.
1
This test method is under the jurisdiction of ASTM Committee E35 on
NOTE 1—Control of bacterial spoilage of similar products can be
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
evaluated by Test Method E723.
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
NOTE 2—Slimicides for control of fungal or bacterial slime can be
Current edition approved April 1, 2010. Published May 2010. Originally
evaluated by Test Method E1839.
approvedin1982.Lastpreviouseditionapprovedin2005asE875 – 00(2005).DOI:
10.1520/E0875–10.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
Standards volume information, refer to the standard’s Document Summary page on 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
the ASTM website. www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E875 − 10
6. Apparatus 7.4.1 Spore Suspending Media and Container—Milk dilu-
4
tion bottles containing 100 mL Butterfield Buffer with solid
6.1 Two Balances— One should be sensitive to 0.1 g at a
glass beads, for preparing sterile spore suspensions.
load of 200 g with a platform to accommodate bottles being
7.4.2 Culture Media, slants of the selec
...

This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E875–00 (Reapproved 2005) Designation: E875 – 10
Standard Test Method for
Efficacy of Fungal Control Agents as Preservatives for
1
Aqueous-Based Products Used in the Paper Industry
This standard is issued under the fixed designation E875; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This laboratory test method is used to determine the efficacy of a fungal control agent to prevent spoilage of in-process
aqueous-based products used in the paper industry.
1.2 For information on bacterial control agents, see Test Method E723.
1.3 It is the responsibility of the investigator to determine whether good laboratory practices (GLP) are required and to follow
them when appropriate (see 40 CFR 160)160).
1.4 A knowledge of microbiological techniques is required for these procedures.
1.5
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water E599Test Method for Efficacy of Slimicides for the Paper Industry--Fungal Slime
0
E600Test Method for Efficacy of Slimicides for the Paper Industry--Bacterial Slime
E723 Test Method for Efficacy of Antimicrobials as Preservatives for Aqueous-Based Products Used in the Paper Industry
(Bacterial Spoilage) Test Method for Efficacy of Antimicrobials as Preservatives for Aqueous-Based Products Used in the
Paper Industry (Bacterial Spoilage)
E1839 Test Method for Efficacy of Slimicides for the Paper IndustryBacterial and Fungal Slime
2.2 Federal Standard:
3
40 CFR 160 Good Laboratory Practice Standards
3. Terminology
3.1 Definitions of Terms Specific to This Standard:
3.1.1 fungal control agent, n—an agent that either kills or prevents growth of fungi and either kills or prevents the germination
of fungal spores. This term is applied to chemical biocidal or biostatic agents.
3.1.2 preservative, n—chemical agent used to prevent microbial soilage of products due to microbial growth.
4. Summary of Test Method
4.1 Aqueous material to be preserved is inoculated with an appropriate fungal inoculum followed by addition of a concentration
of fungal control agent that will kill the fungi or prevent their growth for a desired period of time, or both. In addition, the agent
will also prevent fungal spore germination. Fungal growth is determined by visible signs of deterioration in the test sample, and
by obtaining fungal numbers and comparing them to a sample without any fungal control agent.The proper level of fungal control
agent is one that prevents product deterioration and reduces and keeps the organisms to an acceptable level in the test material,
as determined by the tester or user.
1
This test method is under the jurisdiction ofASTM Committee E35 on Pesticides andAlternative ControlAgents and is the direct responsibility of Subcommittee E35.15
on Antimicrobial Agents.
Current edition approved Nov.April 1, 2005.2010. Published November 2005.May 2010. Originally approved in 1982. Last previous edition approved in 20002005 as
E875 – 00(2005). DOI: 10.1520/E0875-00R05.10.1520/E0875–10.
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
E875 – 10
5. Significance and Use
5.1 This test method should be used to determine if a fungal control agent is effective to preserve pigment suspensions, dye
solutions, pulp slurries, starch solutions, polymers, sizing agents, latex emulsions, and other specific aqueous-based materials used
in the pap
...

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