ASTM E1839-20
(Practice)Standard Practice for Efficacy of Slimicides for the Paper Industry—Bacterial and Fungal Slime
Standard Practice for Efficacy of Slimicides for the Paper Industry—Bacterial and Fungal Slime
SIGNIFICANCE AND USE
5.1 This practice is to be used to determine if a slime control agent has application in the paper industry for control of bacterial or fungal slime/biofilm.
5.2 This practice is run in acid, alkaline, or acid and alkaline conditions to determine the efficacy of the slime control agent.
5.3 The test conditions may be modified to reflect intended use patterns in typical paper mill systems, including use of actual paper mill furnish.
SCOPE
1.1 This practice presents a procedure to evaluate the efficacy of slimicides for the control of bacterial and fungal slimes in paper mill systems and their counterparts.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (40 CFR 160).
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1839 − 20
Standard Practice for
Efficacy of Slimicides for the Paper Industry—Bacterial and
1
Fungal Slime
This standard is issued under the fixed designation E1839; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2.2 TAPPI Standard:
3
T 205Forming Handsheets for Physical Tests of Pulp
1.1 This practice presents a procedure to evaluate the
2.3 CFR Standard:
efficacy of slimicides for the control of bacterial and fungal
4
40 CFR Part 160Good Laboratory Practice Standards
slimes in paper mill systems and their counterparts.
3. Terminology
1.2 It is the responsibility of the investigator to determine
whether Good Laboratory Practices (GLP) are required and to
3.1 For definitions of terms related to this practice, see
follow them where appropriate (40 CFR 160). Terminology E2756.
3.2 Definitions:
1.3 The values stated in SI units are to be regarded as
3.2.1 furnish, n—pulp slurry fed to a paper machine. The
standard. No other units of measurement are included in this
type of pulp (sulfite, Kraft, mechanical), the source of fiber
standard.
(virgin,recycledincludingpre-orpost-consumerwastepaper),
1.4 This standard does not purport to address all of the
and the pH are used to designate a specific type of furnish.
safety concerns, if any, associated with its use. It is the
3.2.2 microbicide, n—aphysicalorchemicalagentthatkills
responsibility of the user of this standard to establish appro-
microorganisms.
priate safety, health, and environmental practices and deter-
3.2.3 pulp, n—wood separated by chemical or mechanical
mine the applicability of regulatory limitations prior to use.
means into their fibrous components.The pulp is used to make
1.5 This international standard was developed in accor-
paper, paper board, or pulp sheets after specific treatments.
dance with internationally recognized principles on standard-
Hardwoodpulpismadefromtrees,suchasmaplesoroaks,and
ization established in the Decision on Principles for the
softwood pulp is produced from trees, such as pines.
Development of International Standards, Guides and Recom-
3.2.4 pulp slurry, n—an aqueous combination of cellulosic
mendations issued by the World Trade Organization Technical
fibers, fillers, and other additives used for specific grades of
Barriers to Trade (TBT) Committee.
paper.
3.2.5 slime, n—biofilm.
2. Referenced Documents
3.2.6 slimicides, n—chemical agent added during pulp and
2
2.1 ASTM Standards:
paper processing to reduce the growth of slime-forming
D1193Specification for Reagent Water
microorganisms.
E1054Test Methods for Evaluation of Inactivators of Anti-
microbial Agents
4. Summary of Practice
E2756Terminology Relating toAntimicrobial andAntiviral
4.1 Bacterial cells or fungal spores are added to acid or
Agents
alkaline pulp slurries, or both, treated with slimicides to
6 7
achieve final concentrations of 2×10 to1×10 bacteria/mL
5 6
and/or 10 to 10 fungal spores/mL, and incubated at appro-
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides,
priatetemperaturefordeterminedtimeperiods.Aliquotsofthe
Antimicrobials, and Alternative Control Agentsand is the direct responsibility of
test suspension are then neutralized, plated onto bacterial or
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Jan. 1, 2020. Published March 2020. Originally
approved in 1996. Last previous edition approved in 2013 as E1839–13. DOI:
3
10.1520/E1839-20. Available from TechnicalAssociation of the Pulp and Paper Industry (TAPPI),
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or 15 Technology Parkway South, Norcross, GA 30092, http://www.tappi.org.
4
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
Standards volume information, refer to the standard’s Document Summary page on 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
the ASTM website. www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1839 − 20
fungal medium, and observed for growth. Results with biocide 6.13 Colony counter, manual, such as Quebec, Buck, and
are compared to results without biocide (control). Wolfhuegel, or proven colony image analyzer (electronic/
scanner type) are suitable for counting pl
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1839 − 13 E1839 − 20
Standard Test Method Practice for
Efficacy of Slimicides for the Paper Industry—Bacterial and
1
Fungal Slime
This standard is issued under the fixed designation E1839; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method presents a procedure to evaluate the efficacy of slimicides for the control of bacterial and fungal slimes
in paper mill systems and their counterparts.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow
them where appropriate (40 CFR 160).
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
2.2 TAPPI Standard:
3
T 205 Forming Handsheets for Physical Tests of Pulp
2.3 CFR Standard:
4
40 CFR Part 160 Good Laboratory Practice Standards
1
This test method practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agentsand is the direct responsibility
of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2013Jan. 1, 2020. Published May 2013March 2020. Originally approved in 1996. Last previous edition approved in 20072013 as
E1839 – 07.E1839 – 13. DOI: 10.1520/E1839-13.10.1520/E1839-20.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from Technical Association of the Pulp and Paper Industry (TAPPI), 15 Technology Parkway South, Norcross, GA 30092, http://www.tappi.org.
4
Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1839 − 20
3. Terminology
3.1 For definitions of terms related to this practice, see Terminology E2756.
3.2 Definitions:
3.2.1 furnish, n—pulp slurry fed to a paper machine. The type of pulp (sulfite, Kraft, mechanical), the source of fiber (virgin,
recycled including pre- or post-consumer waste paper), and the pH are used to designate a specific type of furnish.
3.2.2 microbicide, n—a physical or chemical agent that kills microorganisms.
3.2.3 pulp, n—wood separated by chemical or mechanical means into their fibrous components. The pulp is used to make paper,
paper board, or pulp sheets after specific treatments. Hardwood pulp is made from trees, such as maples or oaks, and softwood
pulp is produced from trees, such as pines.
3.2.4 pulp slurry, n—an aqueous combination of cellulosic fibers, fillers, and other additives used for specific grades of paper.
3.2.5 slime, n—biofilm.
3.2.6 slimicides, n—chemical agent added during pulp and paper processing to reduce the growth of slime-forming
microorganisms.
4. Summary of Test Method
4.1 Bacterial cells or fungal spores are added to acid or alkaline pulp slurries, or both, treated with slimicides to achieve final
6 7 5 6
concentrations of 2 × 10 to 1 × 10 bacteria/mL and/or 10 to 10 fungal spores/mL, and incubated at appropriate temperature for
determined time periods. Aliquots of the test suspension are then neutralized, plated onto bacterial or fungal medium, and observed
for growth. Results with biocide are compared to results without biocide (control).
4.2 As a performance standard, an effective slimicide is one that shows a continued reduction in bacterial and fungal counts
relative to the control over the duration of the test.
5. Significance and Use
5.1 This test method is to b
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