ASTM E1428-99(2009)
(Test Method)Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptoverticillium reticulum (A Pink Stain Organism)
Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by <med><i>Streptoverticillium reticulum</i></i> (A Pink Stain Organism)
SIGNIFICANCE AND USE
This test method provides a technique for evaluating antimicrobials in or on polymeric solids against staining by Streptoverticillium reticulum, and should assist in the prediction of performance of treated articles under actual field conditions.
Conditioning of the specimens, such as exposure to leaching, weathering, and heat treatment, may have significant effects on performance of antimicrobials against staining. Determination of these effects is not included in this test method.
SCOPE
1.1 This test method is used for determining the performance of antimicrobial agents used in or on synthetic polymeric solids against staining by the actinomycete Streptoverticillium reticulum. This organism has been chosen as an indicator organism, although other organisms have been known to cause undesirable staining in polymeric solids.
1.2 This test method is not suitable for evaluating dark-pigmented test samples.
1.3 A knowledge of microbiological techniques is recommended for these procedures.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
General Information
Relations
Buy Standard
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1428 − 99(Reapproved 2009)
Standard Test Method for
Evaluating the Performance of Antimicrobials in or on
Polymeric Solids Against Staining by Streptoverticillium
reticulum (A Pink Stain Organism)
This standard is issued under the fixed designation E1428; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 Conditioning of the specimens, such as exposure to
leaching, weathering, and heat treatment, may have significant
1.1 This test method is used for determining the perfor-
effects on performance of antimicrobials against staining.
mance of antimicrobial agents used in or on synthetic poly-
Determination of these effects is not included in this test
meric solids against staining by the actinomycete Streptover-
method.
ticillium reticulum. This organism has been chosen as an
indicatororganism,althoughotherorganismshavebeenknown
4. Interferences
to cause undesirable staining in polymeric solids.
4.1 An interference may be caused by contamination of
1.2 This test method is not suitable for evaluating dark-
plates and agar by unwanted organisms that settle from the
pigmented test samples.
environment.
1.3 A knowledge of microbiological techniques is recom-
4.2 Dark pigments mask observation of the pink stain.
mended for these procedures.
1.4 The values stated in SI units are to be regarded as
5. Apparatus
standard. No other units of measurement are included in this
5.1 Petri dishes, 100 mm diameter.
standard.
NOTE 1—Presterilized and disposable plastic petri dishes are available
1.5 This standard does not purport to address all of the
from most laboratory supply houses.
safety concerns, if any, associated with its use. It is the
5.2 Cotton swabs, sterile.
responsibility of the user of this standard to establish appro-
priate safety and health practices and determine the applica-
5.3 Incubator—Incubating equipment for this test method
bility of regulatory limitations prior to use.
shall maintain a temperature of 29 6 1°C.
5.4 Autoclave.
2. Summary of Test Method
5.5 Sterilizer, ethylene oxide (optional).
2.1 Test specimens are placed on an agar surface inoculated
with Streptoverticillium reticulum and incubated. After
6. Reagents and Materials
incubation, test specimens are rated visually by percentage of
sample area stained.
6.1 Yeast Malt Extract Agar (ISP Medium 2) —Prepare this
medium according to manufacturer’s directions.
3. Significance and Use
6.2 Inoculum—Streptoverticillium reticulum ATCC 25607.
3.1 This test method provides a technique for evaluating
Maintain stock cultures on yeast malt extract agar. The stock
antimicrobials in or on polymeric solids against staining by
may be kept for not more than 12 months at approximately 3
Streptoverticillium reticulum, and should assist in the predic-
to 10°C. Subcultures, incubated at 29 6 1°C for 7 to 14 days,
tion of performance of treated articles under actual field
shall be used for inoculation.
conditions.
The sole source of supply of Bacto-Yeast Malt ExtractAgar, Stock No. 0770-01
This test method is under the jurisdiction of ASTM Committee E35 on known to the committee at this time is Difco Labs, P.O. Box 1058A, Detroit, MI
Pesticides and Alternative Control Agentsand is the direct responsibility of Sub- 28232. If you are aware of alternative suppliers, please provide this information to
committee E35.15 on Antimicrobial Agents. ASTM International Headquarters. Your comments will receive careful consider-
Current edition approved Oct. 1, 2009. Published November 2009. Originally ation at a meeting of the responsible technical committee, which you may attend.
approved in 1991. Last previous edition approved in 2004 as E1428 – 99(2004). American Type Culture Collection, 10801 University Blvd., Manassas, VA
DOI: 10.1520/E1428-99R09. 20110.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E1428 − 99 (2009)
7. Test Specimens 8.5 Incubation—Cover the inoculated test plates containing
the specimens and incubate at 29 6 1°C for 14 days.
7.1 From each test unit (Note 2), cut duplicate 0.75 in.
diameter discs. If the test unit is of different construction on
NOTE 3—Covered petri dishes containing yeast malt
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.