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ASTM E1054-02

(Test Method)

Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents

Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents

SCOPE
1.1 These methods are used to determine the effectiveness of procedures and agents for inactivating (neutralizing, quenching) the microbiocidal properties of antimicrobial agents and to ensure that no components of the neutralizing procedures and agents, themselves, exert an inhibitory effect on microorganisms targeted for recovery.
Note 1—Knowledge of microbiological and statistical techniques is required for these procedures. These methods are not applicable to testing with viruses (see Test Method E 1482).
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
09-May-2002
ICS
11.080.20 - Disinfectants and antiseptics
71.100.35 - Chemicals for industrial and domestic disinfection purposes
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

Relations

Replaced By
ASTM E1054-08 - Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents
Effective Date
01-Apr-2008
Referred By
ASTM E3160-18 - Standard Test Method for Quantitative Evaluation of the Antibacterial Properties of Porous Antibacterial Treated Articles
Effective Date
10-May-2002
Referred By
ASTM E2966-14(2019) - Standard Test Method for Quantitative Assessment of Sanitizing Solutions for Carpet
Effective Date
10-May-2002
Referred By
ASTM E2752-10(2015) - Standard Guide for Evaluation of Residual Effectiveness of Antibacterial Personal Cleansing Products
Effective Date
10-May-2002
Referred By
ASTM E2799-22 - Standard Test Method for Testing Disinfectant Efficacy against <emph type="bdit">Pseudomonas aeruginosa</emph> Biofilm using the MBEC Assay
Effective Date
10-May-2002
Referred By
ASTM E2315-23 - Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure
Effective Date
10-May-2002
Referred By
ASTM E2406-16 - Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations
Effective Date
10-May-2002
Referred By
ASTM E2111-12(2018) - Standard Quantitative Carrier Test Method to Evaluate the Bactericidal, Fungicidal, Mycobactericidal, and Sporicidal Potencies of Liquid Chemicals
Effective Date
10-May-2002
Referred By
ASTM E640-06(2019) - Standard Test Method for Preservatives in Water-Containing Cosmetics
Effective Date
10-May-2002
Referred By
ASTM E3092-18 - Standard Practice for Evaluating Efficacy of Vaporous Decontaminants on Materials Contaminated with <emph type="bdit">Bacillus</emph> Spores and Contained Within 0.2&#xb5;m Filter-Capped Tubes
Effective Date
10-May-2002
Referred By
ASTM E2870-19 - Standard Practice for Evaluating Relative Effectiveness of Antimicrobial Handwashing Formulations using the Palmar Surface and Mechanical Hand Sampling
Effective Date
10-May-2002
Referred By
ASTM E645-18 - Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
Effective Date
10-May-2002
Referred By
ASTM E1874-22e1 - Standard Practice for Recovery of Microorganisms From Skin using the Cup Scrub Technique
Effective Date
10-May-2002
Referred By
ASTM E3178-18 - Standard Practice for Evaluating Static and Cidal Chemical Decontaminants against <emph type="bdit">Bacillus</emph> Spores using Centrifugal Filtration Tubes
Effective Date
10-May-2002
Referred By
ASTM E2274-16 - Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants
Effective Date
10-May-2002

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ASTM E1054-02 - Standard Test Methods for Evaluation of Inactivators of Antimicrobial AgentsASTM E1054-02 - Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents
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ASTM E1054-02 - Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E1054–02
Standard Test Methods for
1
Evaluation of Inactivators of Antimicrobial Agents
This standard is issued under the fixed designation E 1054; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 antimicrobial effectiveness evaluation—a determina-
tion of microbiocidal properties of an antimicrobial agent by
1.1 These methods are used to determine the effectiveness
methods, such as Test Methods E 645 and E 1115.
of procedures and agents for inactivating (neutralizing,
3.1.3 CFU/mL—colony-forming units of a microorganism
quenching) the microbiocidal properties of antimicrobial
per millilitre of fluid.
agents and to ensure that no components of the neutralizing
3.1.4 neutralizer—a procedure or chemical agent used to
proceduresandagents,themselves,exertaninhibitoryeffecton
inactivate,neutralize,orquenchthemicrobiocidalpropertiesof
microorganisms targeted for recovery.
an antimicrobial agent.
NOTE 1—Knowledge of microbiological and statistical techniques is
3.1.5 neutralizer effectiveness—a neutralizer’s ability to
required for these procedures. These methods are not applicable to testing
inactivate,neutralize,orquenchthemicrobiocidalpropertiesof
with viruses (see Test Method E 1482).
an antimicrobial agent.
1.2 This standard does not purport to address all of the
3.1.6 neutralizer toxicity—any inhibitory effects a neutral-
safety concerns, if any, associated with its use. It is the
izer may have on the survival of a microbial population.
responsibility of the user of this standard to establish appro-
3.1.7 test material control—an evaluation of the activity of
priate safety and health practices and determine the applica-
an test material in reducing a known population of microor-
bility of regulatory limitations prior to use.
ganisms.
3.1.8 test organism viability—the population or viability of
2. Referenced Documents
a challenge microorganism used in a neutralization assay.
2
2.1 ASTM Standards:
E 645 Test Method for Effectiveness of Microbicides Used 4. Summary of Test Methods
in Cooling Systems
NOTE 2—The neutralization test method selected must be identical to
E 1115 Test Method for Evaluation of Surgical Hand Scrub
the method used in the antimicrobial effectiveness evaluation.
Formulations
4.1 Neutralization Assay with Recovery on Solid Medium—
E 1482 Test Method for Neutralization of Virucidal Agents
Neutralization assay for antimicrobial effectiveness tests that
in Virucidal Effectiveness Evaluations
recover and quantify microorganism populations on solid
(agar) media. This method is appropriate for antimicrobial
3. Terminology
agents that can be chemically inactivated or diluted to sub-
3.1 Definitions of Terms Specific to This Standard:
inhibitory levels.
3.1.1 antimicrobial agent—a test formulation, chemical
4.2 Neutralization Assay with Recovery in Liquid
compound, or product designed to prevent the growth of
Medium—Neutralization assay for antimicrobial effectiveness
microbes either by inhibiting growth or destroying the mi-
tests that recover surviving microorganism populations in
crobe.
liquid media for a growth/no growth determination. This
method is appropriate for antimicrobial agents that can be
chemically inactivated or diluted to sub-inhibitory levels.
1
These test methods are under the jurisdiction of ASTM Committee E35 on
4.3 Neutralization Assay with Recovery by Membrane
Pesticides and are the direct responsibility of Subcommittee E35.15 on Antimicro-
Filtration—Neutralization assay for antimicrobial effective-
bial Agents.
ness tests that recover and quantify microorganism populations
Current edition approved May 10, 2002. Published August 2002. Originally
published as E 1054 – 85. Last previous edition E 1054 – 91.
by using membrane filtration. This method is appropriate for
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
antimicrobial agents that cannot be chemically inactivated or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
diluted to sub-inhibitory levels. This method should not be
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. usedwhendifficultiesareincurredduringthefiltrationprocess.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
E1054–02
5. Significance and Use brane filter unit should be suitable for testing the antimicrobial
agent and recovery of the microorganisms.
5.1 The effectiveness of antimicrobial agents such
...

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ASTM E1482-23(Practice)
Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization

SIGNIFICANCE AND USE
5.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and before the inoculation of these mixtures into host systems for assay of viral infectivity.  
5.2 The purpose of the practice is to reduce the concentration of the cytotoxic properties of the test product and neutralizers in order to permit the evaluation of viral infectivity at dilutions that would otherwise be toxic to the host cells.  
5.3 The practice is applicable to the testing of liquid, pre-saturated towelettes, and pressurized disinfectant products, as well as handwash/rub products.  
Note 3: When testing products, the ability of the solution to pass through the column must be verified prior to testing. Certain products with high viscosities are unable to pass through columns. If the product is determined to be too viscous, alternative neutralization methods should be employed.  
5.4 This practice is compatible with organic soil loads, hard water, disinfectants containing organic solvents, and chemical neutralizers.
SCOPE
1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.
Note 1: Gel filtration columns may impact virus titer and their use should be taken into consideration when selected for use.  
1.2 This practice should be performed only by persons trained in virology techniques.  
1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of centrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.  
1.4 This practice will aid in the reduction, but not necessarily elimination, of test product toxicity while preserving the titer of the input virus.  
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ASTM E1054-22(Practice)
Standard Practices for Evaluation of Inactivators of Antimicrobial Agents

SIGNIFICANCE AND USE
5.1 The effectiveness of antimicrobial agents incorporated into disinfectants, sanitizers, and antiseptics is measured by their ability to kill microorganisms within a specified contact time. Hence, accurate determination of antimicrobial effectiveness requires complete and immediate inactivation (neutralization) of the antimicrobial agent. Inefficient or incomplete neutralization will permit killing or inactivation of microorganisms to continue beyond the experimental exposure time, resulting in an overestimation of antimicrobial activity.  
5.2 The neutralization methods commonly used in antimicrobial effectiveness evaluations are chemical inactivation, dilution, and filtration. All critical parameters of an antimicrobial effectiveness evaluation—for example, media, equipment, microorganism(s), and temperature of solutions—must be duplicated in the performance of selected neutralization procedure.  
5.3 The neutralization evaluation must include at least three replications (five replications in Section 9) so that a statistical analysis of the microbial recovery data can be performed. The number of replicates used in the evaluation depends on the statistical significance required for the expected results, the variability encountered in the data, and the relative effectiveness of the neutralization procedure.  
5.4 A limitation of these evaluation procedures is that they use microorganisms that have not been exposed to an antimicrobial agent. Under experimental conditions, cells exposed to neutralization procedures are likely to be damaged to different degrees by the antimicrobial agent. Sublethal injury may be a factor in recovery, and the effect of the neutralization procedure on recovery of injured organisms should be examined. This method is not intended to assess recovery of injured organisms.
Note 3: Ideally, all microorganisms used in the antimicrobial effectiveness evaluation should be tested in the neutralization assay. However, representative organism...
SCOPE
1.1 These test procedures are used to determine the effectiveness of methodologies procedures and materials intended for inactivating (neutralizing, quenching) the microbicidal properties of antimicrobial agents; to ensure that no components of the neutralizing procedures and materials, themselves, exert an inhibitory effect on microorganisms targeted for recovery; and to demonstrate that the antimicrobial chemistry tested is microbicidal.  
1.2 Knowledge of microbiological and statistical techniques is required for these procedures.
Note 1: These methods are not suitable when testing the virucidal activity of microbicides (see Test Method E1482).  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
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ASTM E1153-22(Test Method)
Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate, Hard, Nonporous Non-Food Contact Surfaces

SIGNIFICANCE AND USE
4.1 This test method shall be used to determine if a chemical intended for use as a non-food contact sanitizer or as a one-step cleaner-sanitizer provides percent reductions of the selected test organisms on treated carriers as compared to control.
SCOPE
1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous, non-food contact surfaces against Staphylococcus aureus, or Klebsiella pneumoniae or Klebsiella aerogenes, or a combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When utilizing test surfaces not described herein (see Test Method E2274) or when evaluating spray-based or towelette-based antimicrobial products, modifications may also be required.  
1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner-sanitizer formulations recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces.  
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Standard Test Method for Determining the Bacteria-Eliminating Effectiveness of Healthcare Personnel Hand Rub Formulations Using Hands of Adults

SIGNIFICANCE AND USE
5.1 Hand hygiene is considered one of the most important measures for preventing the spread of infectious microorganisms. Hand rubs reduce the microbial load on the hands without the use of soap and water, and are thus an important tool in the practice of good hand hygiene. Alcohol-based hand rubs are recommended in healthcare settings for use on hands that are not visibly soiled. They are formulated to be applied full strength to dry hands, “rubbed in” until dry, and are not rinsed off.  
5.2 This test method is designed specifically to evaluate hand rubs for efficacy in eliminating bacteria from experimentally-contaminated hands. It is designed as an alternative to Test Method E1174, which was intended primarily to evaluate antimicrobial handwashing agents that are lathered with the aid of water and then rinsed off. When using Test Method E1174 to evaluate hand rubs, inadequate drying of the hands after contamination dilutes the test material and can compromise activity, to result in an underestimation of effectiveness. Additionally, because hand rubs are not rinsed after product use, activity can be further degraded by build-up of soil from the contaminating broth and inactivated challenge bacteria on the hands.  
5.2.1 In this method, application to the hands of a small volume of high-titer test bacteria suspension minimizes soil load such that the skin is completely dry prior to application of the test material. Further, by applying the bacterial suspension only prior to those test material application cycles followed by sampling, excessive buildup of killed bacteria on the hands is avoided, and the potential impact of non-volatile test product ingredients on bacteria-eliminating effectiveness after ten consecutive applications can be specifically assessed.  
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1.1 This test method is designed to determine the activity of healthcare personnel hand rubs, (also known as hand rubs, hygienic hand rubs, hand sanitizers, or hand antiseptics) against transient microbial skin flora on the hands after a single application and after repeated applications.  
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Standard Practice for Evaluating Static and Cidal Chemical Decontaminants against Bacillus Spores using Centrifugal Filtration Tubes

SIGNIFICANCE AND USE
5.1 The practice can be used to evaluate coupon materials of any composition, insofar as the coupon can be small enough to fit inside filter units mentioned in 4.1.  
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5.2.1 Quantitative—The total number of spores per coupon is determined by dilution-plating, and all spores remaining on the coupon are assayed for activity in the extraction tube to provide confidence that all the spores were accounted for.  
5.2.2 Statistical Confidence—The use of five independent preparations of spore inocula for a statistical n of 5.  
5.2.3 Sensitivity—Allows for complete detection of all culturable spores inoculated on a coupon, including the spores that remain attached to the coupon.
5.2.3.1 The limit of detection is dependent on the culturability of fully matured spores to germinate, outgrow and divide in the presence of the extraction medium (1% tryptic soy broth, 100 mM L-Alanine, 1 mM inosine, 0.05% Tween 80) and/or on tryptic soy agar.
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5.2.4 Safety—Inoculated coupons are contained within filter units.  
5.2.5 Simplicity of Testing—Tests and extractions are performed in the same filter unit to minimize coupon handling steps.  
5.2.6 Scalable and Rapid—A maximum of 36 samples can be processed in 1 h by two technicians; a total of 300 samples have been processed by six technicians in 5 h (1, 2).  
5.2.7 Wide application for numerous Bacillus species and strains. The method has also been modified and used for vegetative bacteria and viruses as well (1, 2).
SCOPE
1.1 This practice is used to quantify the efficacy of liquid or solid decontaminants on Bacillus spores dried on the surface of coupons made from porous and non-porous materials. This practice can distinguish between bactericidal and bacteriostatic chemicals within decontamination mixtures. This is important because many decontaminants contain both reactive compounds and high concentrations of bacteriostatic surfactants. All test samples are directly compared to pre-neutralized controls, un-inoculated negative growth controls, and solution controls on the same day as the test in order to increase practical confidence in the inactivation data.  
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ASTM E3092-18(Practice)
Standard Practice for Evaluating Efficacy of Vaporous Decontaminants on Materials Contaminated with Bacillus Spores and Contained Within 0.2µm Filter-Capped Tubes

SIGNIFICANCE AND USE
5.1 The practice can be used to evaluate coupon materials of any composition, insofar as the coupon can be prepared small enough to fit inside a 50-ml conical tube.  
5.2 This practice defines procedures that are quantitative, scalable, rapid, sensitive, safe, reduces consumables, minimizes labor and addresses statistical confidence (1, 2, 4).  
5.2.1 Quantitative—The total number of spores per coupon is determined by dilution-plating, and all spores remaining on the coupon are assayed for activity in the extraction tube to provide confidence that 100 % of spores were assayed.  
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5.2.5 Simplicity of Testing—Tests and extractions are performed in the same 50-ml conical tube to minimize handling steps.  
5.2.6 Scalable and Rapid—A maximum of 36 samples can be processed in 1 h by two technicians; a total of 300 samples have been processed by six technicians in 5 h (1-3).  
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SCOPE
1.1 This practice is used to quantify the efficacy of vaporous decontaminants on Bacillus spores dried on the surface of coupons made from porous and non-porous materials and contained within 0.2µm filter-capped tubes.  
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1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM E1115-11(2017)(Test Method)
Standard Test Method for Evaluation of Surgical Hand Scrub Formulations

SIGNIFICANCE AND USE
5.1 The procedure in this test method should be used to evaluate the activity of the test formulation in reducing the bacterial population of the hands immediately after a single use and to determine persistent activity (inhibition of growth) after 6 h. Optionally, measurements of persistent activity after a 3 h period and measurements of cumulative activity may be made after repetitive uses over a five day period.
SCOPE
1.1 This test method is designed to measure the reduction of microbial flora on the skin. It is intended for determining both immediate and persistent (continuing antimicrobial effect) microbial reductions, after single or repetitive treatments, or both. It may also be used to measure cumulative antimicrobial activity after repetitive treatments.  
1.2 A knowledge of microbiological techniques is required for these procedures.  
1.3 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects (21 CFR, Parts 50 and 56)  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4.1 In this test method, SI units are used for all applications, except for distance, in which case inches are used and SI units follow in parentheses.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
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ASTM E2752-10(2015)(Guide)
Standard Guide for Evaluation of Residual Effectiveness of Antibacterial Personal Cleansing Products

SIGNIFICANCE AND USE
5.1 The procedure is used to evaluate personal cleansing products containing antibacterial ingredients that are intended to reduce the number of organisms on intact skin. It also may be used to demonstrate the effect of residual antibacterial activity by means of inhibition of the proliferation of bacteria on the skin after contact.
SCOPE
1.1 This guide is designed to demonstrate the effectiveness of an antibacterial personal cleansing product in reducing the numbers of a marker organism (representing transients) both immediately and after prolonged exposure to (cleansing) washing when used as recommended under simulated use conditions. The method demonstrates the effect of residual antibacterial activity by means of inhibition of proliferation of bacteria on the skin after the contact period. Antimicrobial activity is compared with a vehicle or to a baseline organism count.  
1.2 A knowledge of microbiological techniques is required for these procedures.  
1.3 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects (21 CFR Parts 50 and 56).  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
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Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization

SIGNIFICANCE AND USE
5.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and before the inoculation of these mixtures into host systems for assay of viral infectivity.  
5.2 The purpose of the practice is to reduce the concentration of the cytotoxic properties of the test product and neutralizers in order to permit the evaluation of viral infectivity at dilutions that would otherwise be toxic to the host cells.  
5.3 The practice is applicable to the testing of liquid, pre-saturated towelettes, and pressurized disinfectant products, as well as handwash/rub products.  
Note 3: When testing products, the ability of the solution to pass through the column must be verified prior to testing. Certain products with high viscosities are unable to pass through columns. If the product is determined to be too viscous, alternative neutralization methods should be employed.  
5.4 This practice is compatible with organic soil loads, hard water, disinfectants containing organic solvents, and chemical neutralizers.
SCOPE
1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.
Note 1: Gel filtration columns may impact virus titer and their use should be taken into consideration when selected for use.  
1.2 This practice should be performed only by persons trained in virology techniques.  
1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of centrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.  
1.4 This practice will aid in the reduction, but not necessarily elimination, of test product toxicity while preserving the titer of the input virus.  
1.5 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM
ASTM E1153-22(Test Method)
Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate, Hard, Nonporous Non-Food Contact Surfaces

SIGNIFICANCE AND USE
4.1 This test method shall be used to determine if a chemical intended for use as a non-food contact sanitizer or as a one-step cleaner-sanitizer provides percent reductions of the selected test organisms on treated carriers as compared to control.
SCOPE
1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous, non-food contact surfaces against Staphylococcus aureus, or Klebsiella pneumoniae or Klebsiella aerogenes, or a combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When utilizing test surfaces not described herein (see Test Method E2274) or when evaluating spray-based or towelette-based antimicrobial products, modifications may also be required.  
1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner-sanitizer formulations recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces.  
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.)  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard may involve hazardous materials, chemicals and microorganisms and should be performed only by persons who have had formal microbiological training.  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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