ASTM E2274-16
(Test Method)Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants
Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants
SIGNIFICANCE AND USE
5.1 The procedure in this test method is used to evaluate the activity of a test reagent (antimicrobial agent/active ingredient) or formulation in the reduction or complete kill of the bacterial population in fabric and wash water following a single wash. The water to fabric ratio in common top loading machines is dynamic and varies by region. The proper water to fabric ratio and temperature for the worse-case scenario should be determined prior to testing. This test method may need to modified if the worse-case scenario is determined to be in top loading high efficiancy washing machine
SCOPE
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditional top-loading automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.
Note 1: This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading or top-loading, low water high efficiency automatic clothes washing operations.
1.2 This test method is intended to compliment Test Method E2406 and is to be used in the cases where this test method is determined to be the worse case scenario for product usage.
1.3 Knowledge of microbiological techniques is required for these procedures.
1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.)
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
Note 2: In this test method, metric units are used for all applications, except for distance in which case inches are used.
1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E2274 − 16
Standard Test Method for
1
Evaluation of Laundry Sanitizers and Disinfectants
This standard is issued under the fixed designation E2274; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
1.1 This test method is designed to evaluate sanitizing/ 2.1 ASTM Standards:
disinfectant laundry detergents/additives for use in traditional D1193Specification for Reagent Water
top-loading automatic clothes washing operations. This test E177Practice for Use of the Terms Precision and Bias in
method is designed predominantly to provide testing with ASTM Test Methods
representative vegetative bacteria but can also be designed to E691Practice for Conducting an Interlaboratory Study to
accommodate the testing of fungi and viruses. Determine the Precision of a Test Method
NOTE 1—This test method does not evaluate sanitizing/disinfectant
E1054Test Methods for Evaluation of Inactivators of Anti-
laundry detergent/additives for use in front-loading or top-loading, low
microbial Agents
water high efficiency automatic clothes washing operations.
E2406Test Method for Evaluation of Laundry Sanitizers
1.2 ThistestmethodisintendedtocomplimentTestMethod
and Disinfectants for Use in High Efficiency Washing
E2406 and is to be used in the cases where this test method is
Operations
determined to be the worse case scenario for product usage.
E2756Terminology Relating toAntimicrobial andAntiviral
Agents
1.3 Knowledge of microbiological techniques is required
2.2 Other Standards:
for these procedures.
AATCC Test Method 70Water Repellency; Tumble Jar
1.4 It is the responsibility of the investigator to determine
3
Dynamic Absorption Test
whether Good Laboratory Practices (GLP) are required and to
OCSPP 810.2400: Disinfectants and Sanitizers for Use on
follow them where appropriate (see section 40 CFR, 160 or as
4
Fabrics and Textiles – Efficacy Data Recommendations
revised.)
5
40 CFR, Part 160, Good Laboratory Practice Standards
1.5 The values stated in SI units are to be regarded as
3. Terminology
standard. No other units of measurement are included in this
standard.
3.1 For definitions of terms used in this test method refer to
Terminology E2756.
NOTE 2—In this test method, metric units are used for all applications,
except for distance in which case inches are used.
3.2 Definitions of Terms Specific to This Standard:
1.6 Appropriate modifications to the test method may be 3.2.1 active antimicrobial ingredient—a substance added to
a formulation intended specifically for the inhibition or inac-
required when the testing organisms are not specified herein.
tivation of microorganisms.
1.7 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
responsibility of the user of this standard to establish appro-
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
priate safety and health practices and determine the applica-
Standards volume information, refer to the standard’s Document Summary page on
bility of regulatory limitations prior to use.
the ASTM website.
3
Available from American Association of Textile Chemists and Colorists
(AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://
1
This test method is under the jurisdiction of ASTM Committee E35 on www.aatcc.org.
4
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct AvailablefromUnitedStatesEnvironmentalProtectionAgency(EPA),William
responsibility of Subcommittee E35.15 on Antimicrobial Agents. Jefferson Clinton Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460,
Current edition approved April 15, 2016. Published May 2016. Originally http://www.epa.gov.
5
approved in 2003. Last previous edition approved in 2009 as E2247–09. DOI: Available from U.S. Government Publishing Office Bookstore 710 North
10.1520/E2274-16. Capitol Street N.W. Washington, DC, http://www.gpo.gov/about/bookstore.htm
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E2274 − 16
3.2.2 antimicrobial agent(s)—an active ingredient designed
to suppress the growth or action of microorganisms.
3.2.3 carrier count control—procedure used to determine
the initial number of microorganisms on a fabric carrier
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2274 − 09 E2274 − 16
Standard Test Method for
1
Evaluation of Laundry Sanitizers and Disinfectants
This standard is issued under the fixed designation E2274; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditional top-loading
automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative
bacteria but can also be designed to accommodate the testing of fungi and viruses.
NOTE 1—This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading, front-loading or top-loading,
low water volume high efficiency automatic clothes washing operations.
1.2 This test method is intended to compliment Test Method E2406 and is to be used in the cases where this test method is
determined to be the worse case scenario for product usage.
1.3 Knowledge of microbiological techniques is required for these procedures.
1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow
them where appropriate (see section 40 CFR, 160 or as revised.)
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
NOTE 2—In this test method, metric units are used for all applications, except for distance in which case inches are used.
1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2406 Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
2.2 Other Documents:Standards:
3
AATCC Test Method 70-199770 Water Repellency; Tumble Jar Dynamic Absorption Test
DIS/TSS 13OCSPP 810.2400 Laundry Additives—Disinfection and Sanitization, U.S. Environmental Protection Agency, Office
of Pesticide Programs, April 1980: Disinfectants and Sanitizers for Use on Fabrics and Textiles – Efficacy Data
4
Recommendations
5
40 CFR, Part 160 , Good Laboratory Practice Standards
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 1, 2009April 15, 2016. Published December 2009May 2016. Originally approved in 2003. Last previous edition approved in 20032009 as
E2247 – 03.E2247 – 09. DOI: 10.1520/E2274-09.10.1520/E2274-16.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from American Association of Textile Chemists and Colorists (AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://www.aatcc.org.
4
Available from United States Environmental Protection Agency (EPA), Ariel Rios William Jefferson Clinton Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460,
http://www.epa.gov.
5
Available from U.S. Government Publishing Office Bookstore 710 North Capitol Street N.W. Washington, DC, http://www.gpo.gov/about/bookstore.htm
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E2274 − 16
3. Terminology
3.1 For definitions of terms used in this test method refer to Terminology E2756.
3.2 Definitions:Definitio
...
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