ASTM E1428-99
(Test Method)Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptoverticillium Reticulum (A Pink Stain Organism)
Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by <i>Streptoverticillium Reticulum</i> (A Pink Stain Organism)
SCOPE
1.1 This test method is used for determining the performance of antimicrobial agents used in or on synthetic polymeric solids against staining by the actinomycete streptoverticillium reticulum. This organism has been chosen as an indicator organism, though other organisms have been known to cause undesirable staining in polymeric solids.
1.2 This test method is not suitable for evaluating dark-pigmented test samples.
1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E 1428 – 99
Standard Test Method for
Evaluating the Performance of Antimicrobials in or on
Polymeric Solids Against Staining by Streptoverticillium
reticulum (A Pink Stain Organism)
This standard is issued under the fixed designation E 1428; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 4. Interferences
1.1 This test method is used for determining the perfor- 4.1 An interference may be caused by contamination of
mance of antimicrobial agents used in or on synthetic poly- plates and agar by unwanted organisms that settle from the
meric solids against staining by the actinomycete Streptover- environment.
ticillium reticulum. This organism has been chosen as an 4.2 Dark pigments mask observation of the pink stain.
indicator organism, although other organisms have been known
5. Apparatus
to cause undesirable staining in polymeric solids.
1.2 This test method is not suitable for evaluating dark- 5.1 Petri dishes, 100 mm diameter.
5.2 Cotton swabs, sterile.
pigmented test samples.
1.3 A knowledge of microbiological techniques is recom- 5.3 Incubator—Incubating equipment for this test method
shall maintain a temperature of 29 6 1°C.
mended for these procedures.
1.4 This standard does not purport to address all of the 5.4 Autoclave.
5.5 Sterilizer, ethylene oxide (optional).
safety problems, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
6. Reagents and Materials
priate safety and health practices and determine the applica-
6.1 Yeast Malt Extract Agar (ISP Medium 2) —Prepare this
bility of regulatory limitations prior to use.
medium according to manufacturer’s directions.
2. Summary of Test Method
6.2 Inoculum—Streptoverticillium reticulum ATCC
25607. Maintain stock cultures on yeast malt extract agar. The
2.1 Test specimens are placed on an agar surface inoculated
with Streptoverticillium reticulum and incubated. After incuba- stock may be kept for not more than 12 months at approxi-
mately 3 to 10°C. Subcultures, incubated at 29 6 1°C for 7 to
tion, test specimens are rated visually by percentage of sample
area stained. 14 days, shall be used for inoculation.
7. Test Specimens
3. Significance and Use
3.1 This test method provides a technique for evaluating 7.1 From each test unit, cut duplicate 0.75 in. diameter
discs. If the test unit is of different construction on each side,
antimicrobials in or on polymeric solids against staining by
Streptoverticillium reticulum, and should assist in the predic- two specimens of each, two face up and two face down, shall
be tested.
tion of performance of treated articles under actual field
conditions. 7.2 A test unit containing no biocide should be included as
a positive stain control.
3.2 Conditioning of the specimens, such as exposure to
leaching, weathering, and heat treatment, may have significant
effects on performance of antimicrobials against staining.
Determination of these effects is not included in this test
method.
Presterilized and disposable plastic petri dishes are available from most
laboratory supply houses.
Bacto-Yeast Malt Extract Agar, Stock No. 0770-01, available from Difco Labs,
This test method is under the jurisdiction of ASTM Committee E-35 on P.O. Box 1058A, Detroit, MI 28232, has been found suitable for this purpose.
Pesticides and is the direct responsibility of Subcommittee E35.15 on Antimicrobial American Type Culture Collection, 10801 University Blvd., Manassas, VA
Agents. 20110.
Current edition approved March 10, 1999. Published July 1999. Originally A test unit is a solid in the form of plastic sheets, films, coated fabrics or similar
published as E 1428–91. Last previous edition E 1428–91. polymeric materials.
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