ASTM E787-81(2011)
(Specification)Standard Specification for Disposable Glass Micro Blood Collection Pipets
Standard Specification for Disposable Glass Micro Blood Collection Pipets
ABSTRACT
This specification covers two dimensionally different (short and long) disposable glass micropipets used primarily to collect whole human blood specimens for clinical analysis and testing. Short and long pipets are available as coated with heparin (Type I) or uncoated (Type II).The pipets shall be fabricated from borosilicate glass, Type I, Class B, or soda lime glass, Type II. Heparin shall be the ammonium salt isolated from the lungs or intestinal mucosa of beef or pork origin and shall meet the specified heparin potency. The physical requirements including design, dimensions, workmanship, color coding, capillarity, fluidity, lot or control number, resistance to centrifugal force, and heparin coating are specified. The following tests shall be performed: capillarity test, fluidity test, sheep plasma test, human whole blood test, resistance to centrifugal force test, and heparin content test. The physical requirements for short Caraway pipet and long Natelson pipet are illustrated as well.
SCOPE
1.1 This specification covers two dimensionally different disposable glass micropipets used primarily to collect whole human blood specimens for clinical analysis and testing. They are available as coated with heparin or uncoated.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E787 −81 (Reapproved 2011)
Standard Specification for
Disposable Glass Micro Blood Collection Pipets
This standard is issued under the fixed designation E787; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1. Scope 5. Materials and Manufacture
1.1 This specification covers two dimensionally different
5.1 Glass—The pipets shall be fabricated from borosilicate
disposable glass micropipets used primarily to collect whole
glass, Type I, Class B, or soda lime glass, Type II, in
human blood specimens for clinical analysis and testing. They
accordance with Specification E438.
are available as coated with heparin or uncoated.
5.2 Heparin—shall be the ammonium salt isolated from the
lungs or intestinal mucosa of beef or pork origin. The heparin
2. Referenced Documents
potency shall be 1 mg of ammonium heparin compound which
2.1 ASTM Standards:
is equal to at least 100 USP units.
E438 Specification for Glasses in Laboratory Apparatus
6. Physical Requirements
3. Terminology
6.1 Design—The disposable glass micro blood collection
3.1 Definitions of Terms Specific to This Standard:
pipets, both short and long, shall be straight and pulled to a
3.1.1 disposable micropipets—in accordance with this
tapered point at one end.Any cross section of the pipets, taken
specification and the expected product performance expressed
in a plane perpendicular to the longitudinal axis, shall be
in this standard, those pipets which are to be used one time
circular. The pipets shall be lightly firepolished at both ends
only. Any institution or individual who reuses a disposable
with no run-in and possess color bands to denote presence or
pipet must bear full responsibility for its safety and effective-
absence of heparin content.
ness.
6.2 Dimensions:
6.2.1 The short Caraway pipet shall be approximately 75
4. Classification
mm long and 4 mm in outside diameter. The pipet shall hold a
4.1 This specification covers two dimensionally different
liquid volume of 310 to 470 µL. The tapered point length and
disposable glass pipets as follows:
tip orifice opening shall be as specified in Fig. 1.
4.1.1 Short Pipet—Approximately 75 mm long and coated
6.2.2 The long Natelson pipet shall be approximately 150
with heparin (Type I) or uncoated (Type II). These are
mm long and 3 mm in outside diameter. The pipet shall hold a
commercially recognized as Caraway pipets.
liquid volume of 220 to 420 µL. The tapered point length and
4.1.2 Long Pipet—Approximately 150 mm long and coated
tip orifice opening shall be as specified in Fig. 2.
with heparin (Type I) or uncoated (Type II). These are
6.3 Workmanship—The pipets, as illustrated in Fig. 1 and
commercially recognized as Natelson pipets.
Fig. 2, shall be free of defects that noticeably detract from their
appearance or impair their serviceability. They shall be free of
lint, or significant foreign matter, loose or embedded when
This specification is under the jurisdiction of ASTM Committee E41 on
viewedundernormalroomlighting.Thetopandtipendsofthe
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
pipets shall be cut at approximately 90° to the pipet axis and
Apparatus.
Current edition approved Dec. 1, 2011. Published December 2011. Originally
shall not be cracked or have jagged ends or chips that enter the
ε1
approved in 1981. Last previous edition approved in 2006 as E787 – 81 (2006) .
bore of the pipet.
DOI: 10.1520/E0787-81R11.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
6.4 Color Coding—Each disposable glass micro blood col-
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
lection pipet shall be color-coded to identify the pipet. The
Standards volume information, refer to the standard’s Document Summary page on
heparin-coated pipet (Type 1) shall have a red color band. The
the ASTM website.
Caraway, W. T., and Fanger, H., “Ultramicro Procedures In Clinical
Chemistry,” American Journal of Clinical Pathology , 25, 1955, pp. 316–331.
Natelson, S., Ph.D., Micro-Techniques of Clinical Chemistry, Charles C.
Thomas, Springfield, Ill., 1961, p. 70. The United States Pharmacopeia, 19th Revision, pp. 229–230.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E787−81 (2011)
7.1.1 When using a sealant or plastic closure, the pipets
should not be filled completely to allow for dry space which
will be occupied by the sealant or closure. This step should aid
in preventing pipet leakage when handled or centrifuged.
7.2 Fluidity Test—Test the pipets, both short and long, for
fluidity by using sheep plasma (6.3) or human whole blood
(6.4).
7.3 Sheep Plasma Test:
7.3.1 General—Conduct the test initially by preparing re-
calcified sheep plasma by the following process:
7.3.1.1 Prepare sheep plasma in accordance with the USP
assayforsodiumheparin.Add10mLofpreparedsheepplasma
Capacity: 310 to 470 µL
to 2.0 mL of the 1.0 % calcium chloride solution used in the
Coding: Red band-heparin-coated (Type I)
heparin assay. Mix the sheep plasma and calcium chloride
Blue band-uncoated (Type II)
solution well.
7.3.2 Preparation of Controls—Use samples of both the
Dimensions in millimetres
A Overall length 73–77
plain sheep plasma and recalcified sheep plasma as controls in
B Outside diameter 3.90–4.20
accordance with the following:
C Inside diameter 2.40–2.80
7.3.2.1 Positive Control—Fill an uncoated (that is, nonhepa-
D Inside tip diameter 0.70–1.50
E Length of taper 6–12
rinized) pipet with recalcified sheep plasma.
F Color band location 0–10
7.3.2.2 Negative Control—Fill a coated (that is, heparin-
ized) pipet with plain sheep plasma.
FIG. 1 Caraway Pipet
7.3.3 Procedure—Immediately after the preparation of re-
calcified sheep plasma, fill the pipets by immersing the tips in
the recalcified sheep plasma while holding the pipe
...
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