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ASTM E734-80(2010)

(Specification)

Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)

Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)

ABSTRACT
This specification describes the physical requirements and corresponding test methods for disposable glass blood sample capillary tubes for use in microhematocrit procedures. Covered here are two different types of capillary tubes, namely, Type I (coated with heparin), and Type II (uncoated). The tubes shall be fabricated from Type I, Class B borosilicate glass, or Type II soda lime glass. Conversely, the heparin used for coating Type I tubes shall be of ammonium salt isolated from the lungs or intestinal mucosa of beef or pork origin. The tubes shall conform to specified requirements for design, dimension, workmanship, color coding, and lot or control number. They should also pass the following tests for capillarity, fluidity, sheep plasma, positive and negative controls, human whole blood, heparin potency assay, and resistance to centrifugal force.
SCOPE
1.1 This specification covers disposable glass blood sample capillary tubes for use in microhematocrit procedures.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

General Information

Status
Historical
Publication Date
30-Jun-2010
Technical Committee
E41 - Laboratory Apparatus
Drafting Committee
E41.01 - Laboratory Ware and Supplies
Current Stage
Ref Project

Relations

Replaces
ASTM E734-80(2005) - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)
Effective Date
01-Jul-2010

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ASTM E734-80(2010) - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)ASTM E734-80(2010) - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)
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ASTM E734-80(2010) - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E734 −80(Reapproved 2010)
Standard Specification for
Disposable Glass Blood Sample Capillary Tube
1
(Microhematocrit)
This standard is issued under the fixed designation E734; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5.2 Heparin—Heparin shall be of ammonium salt isolated
from the lungs or intestinal mucosa of beef or pork origin.The
1.1 This specification covers disposable glass blood sample
heparin potency shall be 1 mg of ammonium heparin com-
capillary tubes for use in microhematocrit procedures.
pound which shall be equal to at least 60 United States
1.2 The values stated in SI units are to be regarded as
Pharmacopeia (USP) units. Dry or wet heparin may be applied
standard. No other units of measurement are included in this
to the tube.
standard.
6. Physical Requirements
2. Referenced Documents
6.1 Design—The capillary tubes shall be straight and open
2
2.1 ASTM Standards:
atbothendswithoutliporconstriction.Thecapillarytubemay
E438Specification for Glasses in Laboratory Apparatus belightlyfirepolishedononeend.Theboreofthetubeshallbe
2.2 Other Standard: uniform and not vary in excess of 0.025 mm in 75 mm.
USP XIX United States Pharmacopeia
6.2 Dimensions—Type I and Type II capillary tubes shall
have a length of 75 6 0.5 mm. Inside diameter shall be from
3. Terminology
1.07 to 1.24 mm. Wall thickness shall be 0.20+0.03,−0.02
3.1 Definitions of Terms Specific to This Standard:
mm, as specified in Fig. 1.
3.1.1 disposable capillary tubes, n—in accordance with this
6.3 Workmanship—The capillary tubes shall be free of
specification and the expected product performance expressed
defects that noticeably detract from their appearance or impair
in this standard, those capillary tubes which are to be used one
their serviceability. The capillary tube shall be free of lint, or
time only. Any institution or individual who reuses a dispos-
significant foreign matter, loose or embedded, when viewed
able capillary tube must bear full responsibility for its safety
under normal room lighting. The tube ends shall be cut
and effectiveness.
approximately 90° to the tube axis and shall not be cracked or
have jagged ends or chips that enter the bore of the tubing.
4. Classification
6.4 Color Coding—Each capillary tube shall be color coded
4.1 This specification covers two different disposable glass
to identify the tube as coated with heparin or uncoated.Type I,
sample capillary tubes as follows:
heparin coated, shall have a red band and Type II, uncoated,
Type I—Coated with heparin.
shall have a blue band. The location of the red or blue band
Type II—Uncoated.
shall be as specified in Fig. 1.
5. Materials
6.5 Capillarity—The capillary tube shall be capable of
5.1 Glass—The pipets made to this specification shall be drawing sheep plasma or human whole blood to a level within
fabricated from borosilicate glass, Type I, Class B, or soda- 20 mm from the far end of the tube when tested as specified in
lime glass, Type II, in accordance with Specification E438. 7.1.
6.6 Fluidity(TypeI,Heparinized,only)—Coagulationofthe
sheepplasmaorhumanwholebloodshallnotbeevidentwhen
1
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on viewed under normal room lighting and tested as specified in
Apparatus.
7.2.
CurrenteditionapprovedJuly1,2010.PublishedJuly2010.Originallyapproved
6.7 Lot or Control Number—A lot or control number shall
in 1980. Last previous edition approved in 2005 as E734–80(2005). DOI:
10.1520/E0734-80R10.
be indicated on the capillary tube unit container and on the
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
intermediate package of containers.This lot or control number
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
shall be traceable to the origin (raw material purchases) of the
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. manufacturing record.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E734−80 (2010)
FIG. 1 Type I and Type II Capillary Tubes
6.8 Resistance to Centrifugal Force—Resistance to capil- 7.1.1 When using a sealant, the tube may not be filled
lary tube centrifugal force shall be such that no breakage completelytoallowfordryspacetobeoccupiedbythesealant
results when the tubes are tested as specified in 7.4. and avoid leakage.
6.9 Heparin Coating (Type I, Heparinized, on
...

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