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ASTM E1259-05

(Test Method)

Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C

Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C

SIGNIFICANCE AND USE
Guide D 6469 details the types of problems associated with uncontrolled microbial growth in fuels and fuel systems. Treatment with effective antimicrobial agents is one element of contamination control strategy.
The procedure should be used to evaluate the relative efficacy of microbicides in liquid fuels boiling below 390°C. The effect of environmental conditions, such as a variety of fuel additives, metal surfaces, and climatology, are variables that can be included in specific tests using this protocol.
This practice addresses product performance issues only. Regulatory Agencies restrict and control the use of both pesticides (in the U.S.: 40 CFR 152) and fuel additives (40 CFR 79). Regardless of performance in this method, antimicrobials must only be used in compliance with applicable regulations. Specific industries, for example, the aviation industry, may place further restrictions on chemicals used for fuel treatment.
SCOPE
1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4814, D 6227, and D 6751), system deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
31-Oct-2005
ICS
75.160.20 - Liquid fuels
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

Relations

Replaces
ASTM E1259-01 - Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
Effective Date
01-Nov-2005
Replaced By
ASTM E1259-10 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
Effective Date
01-Oct-2010
Referred By
ASTM E1259-23 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
Effective Date
01-Nov-2005
Referred By
ASTM D7826-23a - Standard Guide for Evaluation of New Aviation Gasolines and New Aviation Gasoline Additives
Effective Date
01-Nov-2005
Referred By
ASTM D7847-22 - Standard Guide for Interlaboratory Studies for Microbiological Test Methods
Effective Date
01-Nov-2005
Referred By
ASTM D6469-20 - Standard Guide for Microbial Contamination in Fuels and Fuel Systems
Effective Date
01-Nov-2005

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ASTM E1259-05 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°CASTM E1259-05 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
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ASTM E1259-05 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1259 – 05
Standard Practice for
Evaluation of Antimicrobials in Liquid Fuels Boiling Below
1
390°C
This standard is issued under the fixed designation E1259; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D5465 Practice for Determining Microbial Colony Counts
from Waters Analyzed by Plating Methods
1.1 This practice is designed to evaluate antimicrobial
D6227 Specification for Grade 82 UnleadedAviation Gaso-
agents for the prevention of microbially influenced deteriora-
line
tion of liquid fuels (as defined by Specification D396, D910,
D6293 Test Method for Oxygenates and Paraffin, Olefin,
D975, D1655, D2069, D2880, D3699, D4814, D6227, and
Naphthene, Aromatic(O-PONA) Hydrocarbon Types in
D6751), system deterioration, or both.
Low-Olefin Spark Ignition Engine Fuels by Gas Chroma-
1.2 Knowledge of microbiological techniques is required
tography
for these procedures.
D6469 Guide for Microbial Contamination in Fuels and
1.3 It is the responsibility of the investigator to determine
Fuel Systems
whether Good Laboratory Practice (GLP) is required and to
D6729 Test Method for Determination of Individual Com-
follow them where appropriate (40 CFR, 160), or as revised.
ponents in Spark Ignition Engine Fuels by 100 Metre
1.4 This standard does not purport to address all of the
Capillary High Resolution Gas Chromatography
safety concerns, if any, associated with its use. It is the
D6733 Test Method for Determination of Individual Com-
responsibility of the user of this standard to establish appro-
ponents in Spark Ignition Engine Fuels by 50-Metre
priate safety and health practices and determine the applica-
Capillary High Resolution Gas Chromatography
bility of regulatory limitations prior to use.
D6751 Specification for Biodiesel Fuel Blend Stock (B100)
2. Referenced Documents for Middle Distillate Fuels
2
D6974 Practice for Enumeration of Viable Bacteria and
2.1 ASTM Standards:
Fungi in Liquid Fuels—Filtration and Culture Procedures
D396 Specification for Fuel Oils
E1326 Guide for Evaluating Nonconventional Microbio-
D910 Specification for Aviation Gasolines
logical Tests Used for Enumerating Bacteria
D975 Specification for Diesel Fuel Oils
2.2 NACE Standard:
D1655 Specification for Aviation Turbine Fuels
3
TM0172 Determining Corrosive Properties of Cargoes in
D2069 Specification for Marine Fuels
4
Petroleum Product Pipelines
D2880 Specification for Gas Turbine Fuel Oils
2.3 Federal Standards:
D3699 Specification for Kerosine
40 CFR, Part 79, Fuels and Fuel Additives Registration
D4057 Practice for Manual Sampling of Petroleum and
5
Regulations
Petroleum Products
40 CFR, Part 152, Pesticide Registration and Classification
D4814 Specification for Automotive Spark-Ignition Engine
5
Procedures
Fuel
3. Terminology
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides
3.1 Definitions of Terms Specific to This Standard:
and is the direct responsibility of Subcommittee E35.15 on Antimicrobial Agents.
3.1.1 antimicrobial, n—see biocide.
Current edition approved Nov. 1, 2005. Published December 2005. Originally
approved in 1988. Last previous edition approved in 2001 as E1259 – 01. DOI:
3.1.2 biocide, n—a physical or chemical agent that kills
10.1520/E1259-05.
living organisms.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
4
the ASTM website. Item No. 21204, available from NACE International, Houston TX.
3 5
Withdrawn. The last approved version of this historical standard is referenced AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
on www.astm.org. 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
E1259 – 05
3.1.2.1 Discussion—Biocides are further classified as bac- 6. Apparatus
tericides (kill bacteria), fungicides (kill fungi), and microbi-
6.1 Colony Counter—Any of several types, for example, a
cides (kill both bacterial and fungi). They are also referred to
Quebec Colony Counter may be used.
as antimicrobials.
6.2 Drums; Steel—208 L (55 gal) 16 ga. steel, open-head
3.1.3 microbially-influenced deterioration,
drum with removable 16 ga. lid fitted with 2.05 cm and 1.90
n—decomposition/degradation of material (fuel) or making
cm threaded ports for venting and sampling.
unsuitable for use, as a result of
...

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ASTM E1259-23(Practice)
Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C

SIGNIFICANCE AND USE
5.1 Guide D6469 details the types of problems associated with uncontrolled microbial growth in fuels and fuel systems. Treatment with effective antimicrobial agents is one element of contamination control strategy.  
5.2 The procedure should be used to evaluate the relative efficacy of microbicides in liquid fuels boiling below 390 °C. The effect of environmental conditions, such as a variety of fuel additives, metal surfaces, and climatology, are variables that can be included in specific tests using this protocol.  
5.3 This practice addresses product performance issues only. Regulatory Agencies restrict and control the use of both pesticides (in the U.S.: 40 CFR 152) and fuel additives (40 CFR 79). Regardless of performance in this method, antimicrobials must only be used in compliance with applicable regulations. Specific industries, for example, the aviation industry, may place further restrictions on chemicals used for fuel treatment.
SCOPE
1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751, and D7467), system deterioration, or both.  
1.2 Knowledge of microbiological techniques is required for these procedures.  
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Standard Test Method for Hydrogen Content of Aviation Turbine Fuels by Low Resolution Nuclear Magnetic Resonance Spectrometry

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5.1 The combustion quality of aviation turbine fuel has traditionally been controlled in specifications by such tests as smoke point (see Test Method D1322), smoke volatility index, aromatic content of luminometer number (see Test Method D1740). Evidence is accumulating that a better control of the quality may be obtained by limiting the minimum hydrogen content of the fuel.  
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1.1 This test method covers the determination of the hydrogen content of aviation turbine fuels.  
1.2 Use Test Methods D4808 or D7171 for the determination of hydrogen in other petroleum liquids.  
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1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific warning statement, see 7.1.  
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ASTM D4808-23(Test Method)
Standard Test Methods for Hydrogen Content of Light Distillates, Middle Distillates, Gas Oils, and Residua by Low-Resolution Nuclear Magnetic Resonance Spectroscopy

SIGNIFICANCE AND USE
5.1 The hydrogen content represents a fundamental quality of a petroleum product that has been correlated with many of the performance characteristics of that product.  
5.2 This test method provides a simple and more precise alternative to existing test methods, specifically combustion techniques (Test Methods D5291) for determining the hydrogen content on a range of petroleum products.
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Test Method  
Petroleum Products  
Boiling Range, °C (°F)
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A  
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15–260 (60–500)  
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ASTM D5188-23(Test Method)
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5.1 This guide is intended for the developers or sponsors of new aviation gasolines or additives to describe the data requirements necessary to support the development of specifications for these new products by ASTM members. The ultimate goal of the data generated in accordance with this guide is to provide an understanding of the performance of the new fuel or additive within the property constraints and compositional bounds of the proposed specification criteria.  
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5.1 This test method provides a rapid and precise elemental measurement with simple sample preparation. Typical analysis times are approximately 4 min to 5 min per sample with a preparation time of approximately 1 min to 3 min per sample.  
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5.1 Some acids can be present in aviation turbine fuels due either to the acid treatment during the refining process or to naturally occurring organic acids. Significant acid contamination is not likely to be present because of the many check tests made during the various stages of refining. However, trace amounts of acid can be present and are undesirable because of the consequent tendencies of the fuel to corrode metals that it contacts or to impair the water separation characteristics of the aviation turbine fuel.  
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5.1 Wear due to excessive friction resulting in shortened life of engine components such as fuel pumps and fuel controls has sometimes been ascribed to lack of lubricity in an aviation fuel.  
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SCOPE
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5.1 The analysis of trace oxygenates in automotive spark-ignition engine fuel has become routine in certain areas to ensure compliance whenever oxygenated fuels are used. In addition, test methods to measure trace levels of oxygenates in automotive spark-ignition fuel are necessary to assess product quality.
SCOPE
1.1 This test method covers the determination of trace oxygenates in automotive spark-ignition engine fuel. The method used is a multidimensional gas chromatographic method using 1,2-dimethoxy ethane as the internal standard. The oxygenates that are analyzed are: methyl-tertiary butyl ether (MTBE), ethyl-tertiary butyl ether (ETBE), diisopropyl ether (DIPE), methanol, tertiary-amyl methyl ether (TAME), n-propanol, i-propanol, n-butanol, i-butanol, tert-butyl alcohol, sec-butyl alcohol, and tert-pentanol. Ethanol is usually not measured as a trace oxygenate since ethanol can be used as the main oxygenate compound in finished automotive spark-ignition fuels such as reformulated automotive spark-ignition fuels. The concentration range of the oxygenates covered in the ILS study was from 10 mg/kg to 2000 mg/kg. In addition this method is also suitable for the measurement of the C5 isomeric alcohols (2-methyl-1-butanol, 2-methyl-2-butanol) present from the fermentation of ethanol.  
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SCOPE
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1.2.1 Exceptions—SI units are provided for all parameters except where there is no direct equivalent such as the units for screw threads, National Pipe Threads/diameters, tubing size, or where there is a sole source of supply equipment specification.  
1.2.2 See also A7.1 for clarification; it does not supersede 1.2 and 1.2.1.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. See Annex A1 for general safety precautions.  
1.4 Table of Contents:    
Section  
Scope  
1  
Referenced Documents  
2  
Terminology  
3  
Summary of Test Method  
4  
Significance and Use  
5  
Apparatus  
6  
Engine Fluids and Cleaning Solvents  
7  
Preparation of Apparatus  
8  
Engine/Stand Calibration and Non-Reference Oil Tests  
9  
Test Procedure  
10  
Calculations, Ratings, and Test Validity  
11  
Report  
12  
Precision and Bias  
13  
Annexes  
Safety Precautions  
Annex A1  
Intake Air Aftercooler  
Annex A2  
The Cummins ISB Engine Build Parts Kit  
Annex A3  
Sensor Locations and Special Hardware  
Annex A4  
External Oil System  
Annex A5  
Cummins Service Publications  
Annex A6  
Specified Units and Formats  
Annex A7  
Oil Analyses  
Annex A8  
Alternate Fuel Approval Process  
Annex A9  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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