ASTM E1259-01

Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C

SCOPE
1.1 This test method is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4818 and D 6227), system deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 09-Apr-2001
• ICS: 75.160.20 - Liquid fuels
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Replaces: ASTM E1259-94 - Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
• Effective Date: 10-Apr-2001
• Replaced By: ASTM E1259-05 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
• Effective Date: 01-Nov-2005
• Referred By: ASTM D6469-20 - Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• Effective Date: 10-Apr-2001
• Referred By: ASTM D7826-23a - Standard Guide for Evaluation of New Aviation Gasolines and New Aviation Gasoline Additives
• Effective Date: 10-Apr-2001
• Referred By: ASTM E1259-23 - Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
• Effective Date: 10-Apr-2001
• Referred By: ASTM D7847-22 - Standard Guide for Interlaboratory Studies for Microbiological Test Methods
• Effective Date: 10-Apr-2001

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E 1259 – 01
Standard Test Method for
Evaluation of Antimicrobials in Liquid Fuels Boiling Below
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390°C
This standard is issued under the fixed designation E 1259; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 2.2 Federal Standards:
40 CFR, Part 79, Fuels and Fuel Additives Registration
1.1 This test method is designed to evaluate antimicrobial
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Regulations
agents for the prevention of microbially influenced deteriora-
40 CFR, Part 152, Pesticide Registration and Classification
tion of liquid fuels (as defined by Specification D 396, D 910,
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Procedures
D 975,D 1655,D 2069,D 2880,D 3699,D 4818andD 6227),
system deterioration, or both.
3. Terminology
1.2 Knowledge of microbiological techniques is required
3.1 Definitions of Terms Specific to This Standard:
for these procedures.
3.1.1 antimicrobials, n—chemical agents that are cidal or
1.3 It is the responsibility of the investigator to determine
static to microorganisms.
whether Good Laboratory Practice (GLP) is required and to
3.1.2 microbial-induced deterioration, n—decomposition/
follow them where appropriate (40 CFR, 160), or as revised.
degradation of material (fuel) or making unsuitable for use, as
1.4 This standard does not purport to address all of the
a result of metabolic activity or the presence of microbes
safety concerns, if any, associated with its use. It is the
3.1.3 microbicide, n—an agent that kills microbes: bacterial
responsibility of the user of this standard to establish appro-
vegetative cells, fungal vegetative cells and spores, algae and
priate safety and health practices and determine the applica-
protozoa. This term is applied to chemical agents that kill
bility of regulatory limitations prior to use.
microbes.
2. Referenced Documents
4. Summary of Test Method
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2.1 ASTM Standards:
4.1 This test method is conducted on a reference fuel, and
D 396 Specification for Fuel Oils
determinines the antimicrobial efficacy under well-defined
D 910 Specification for Aviation Gasolines
conditions that include specific inocula Pseudomonas aerugi-
D 975 Specification for Diesel Fuel Oils
nosa, American Type Culture Collection, (ATCC) No. 33988,
D 1655 Specification for Aviation Turbine Fuels
Hormoconis resinae, ATCC No. 20495, and Yarrowia tropica-
D 2069 Specification for Marine Fuels
lis (formerly Candida tropicalis, ATCC No. 18138; as well as
D 2880 Specification for Gas Turbine Fuels
water/fuel ratios, and time of containment. It is designed for
D 3699 Specification for Kerosine
destructive sampling at regular intervals during bottom water
D 4814 SpecificationforAutomotiveSpark-IgnitionEngine
buildup. This test method allows for impact of fuel/water
Fuel
partitioning and time, on the antimicrobial agent, as well as the
D 6227 Specification for Grade 82 Unleaded Aviation
effect of continual rechallenge. Every 2 weeks, water phase is
Gasoline
increased by 0.25 % while concomitantly, a paired system is
D 6469 Guide to Microbial Contamination in Fuels and
destructively tested. Thus, at 4 weeks, there is an increase in
Fuel Systems
the water phase to 0.5 %, at 6 weeks 0.75 %, and at 8 weeks
1.0 %. At each sampling time interval, treated and untreated
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aliquots are checked for the three types of organisms in the
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides and is the direct responsibility of Subcommittee E35.15 onAntimicrobial
initial inoculum. These counts are coupled with gross obser-
Agents.
vations of each system for biofilm formation and interfacial
Current edition approved April 10, 2001. Published June 2001. Originally
growth.
published as E 1259 – 88. Last previous edition E 1259 – 94.
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
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Standards volume information, refer to the standard’s Document Summary page on Available from the Superintendent of Documents, U.S. Government Printing
the ASTM website. Office, Washington, DC 20402.
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E1259–01
5. Significance and Use Before initiating fuel antimicrobial tests, revitalize each of the
three cultures in accordance with the instructions contained
5.1 Guide D 6469 details the types of problems associated
with each culture.
with uncontrolled microbial growth in fuels and fuel systems.
8.1.2 Maintenance and Preparation of Inocula—All three
Treatment with effective antimicrobial a
...