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ASTM E3152-18

(Guide)

Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

SIGNIFICANCE AND USE
3.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers.  
3.2 Antifungal activity is typically:  
3.2.1 Determination of article susceptibility to fungal colonization,  
3.2.2 Determination of fungistatic activity (qualitative determination of prevented or delayed fungal colonization), and  
3.2.3 Determination of fungicidal/sporicidal activity (quantitative determination of spore kill).  
3.3 The degree of required surface examination varies from gross visual examination to detailed microscopic assessment among these methods.  
3.4 This guide provides an overview of established methods and suggestions for their applicability, with consideration to the type of substrate treated or the type of antifungal treatment being assessed.
SCOPE
1.1 This guide provides information on various test methods currently available to assess antifungal activity on natural or synthetic substrates.  
1.2 Knowledge of microbiological techniques is required for the practice of this guide.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Historical
Publication Date
31-Jan-2018
ICS
11.100.20 - Biological evaluation of medical devices
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

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ASTM E3152-18 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial AgentsASTM E3152-18 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
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ASTM E3152-18 - Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E3152 − 18
Standard Guide for
Standard Test Methods and Practices Available for
Determining Antifungal Activity on Natural or Synthetic
1
Substrates Treated with Antimicrobial Agents
This standard is issued under the fixed designation E3152; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D4141/D4141M Practice for Conducting Black Box and
Solar Concentrating Exposures of Coatings
1.1 This guide provides information on various test methods
D4300 Test Methods for Ability of Adhesive Films to
currently available to assess antifungal activity on natural or
Support or Resist the Growth of Fungi
synthetic substrates.
D4445 Test Method for Fungicides for Controlling Sapstain
1.2 Knowledge of microbiological techniques is required
and Mold on Unseasoned Lumber (Laboratory Method)
for the practice of this guide.
D4576 Test Method for Mold Growth Resistance of Wet
1.3 This standard does not purport to address all of the
Blue and Wet White
safety concerns, if any, associated with its use. It is the
D4783 Test Methods for Resistance of Adhesive Prepara-
responsibility of the user of this standard to establish appro-
tions in Container to Attack by Bacteria, Yeast, and Fungi
priate safety, health, and environmental practices and deter-
D5259 Test Method for Isolation and Enumeration of En-
mine the applicability of regulatory limitations prior to use.
terococci from Water by the Membrane Filter Procedure
1.4 This international standard was developed in accor-
D5590 Test Method for Determining the Resistance of Paint
dance with internationally recognized principles on standard-
Films and Related Coatings to Fungal Defacement by
ization established in the Decision on Principles for the
Accelerated Four-Week Agar Plate Assay
Development of International Standards, Guides and Recom-
D6329 Guide for Developing Methodology for Evaluating
mendations issued by the World Trade Organization Technical
the Ability of Indoor Materials to Support Microbial
Barriers to Trade (TBT) Committee.
Growth Using Static Environmental Chambers
D6469 Guide for Microbial Contamination in Fuels and Fuel
2. Referenced Documents
Systems
2
2.1 ASTM Standards: D6974 Practice for Enumeration of Viable Bacteria and
C1338 Test Method for Determining Fungi Resistance of
Fungi in Liquid Fuels—Filtration and Culture Procedures
Insulation Materials and Facings D7436 Classification System for Unfilled Polyethylene Plas-
D2020 Test Methods for Mildew (Fungus) Resistance of
tics Molding and Extrusion Materials with a Fractional
3
Paper and Paperboard (Withdrawn 2009) Melt Index Using ISO Protocol and Methodology
D3273 Test Method for Resistance to Growth of Mold on the
D7855/D7855M Test Method for Determination of Mold
Surface of Interior Coatings in an Environmental Cham-
Growth on Coated Building Products Designed for Inte-
ber
rior Applications Using an Environmental Chamber and
D3456 Practice for Determining by Exterior Exposure Tests
Indirect Inoculation
the Susceptibility of Paint Films to Microbiological Attack
D7910 Practice for Collection of Fungal Material From
Surfaces by Tape Lift
E1326 Guide for Evaluating Non-culture Microbiological
1 Tests
This guide is under the jurisdiction of ASTM Committee E35 on Pesticides,
Antimicrobials, and Alternative Control Agents and is the direct responsibility of E2111 Quantitative Carrier Test Method to Evaluate the
Subcommittee E35.15 on Antimicrobial Agents.
Bactericidal, Fungicidal, Mycobactericidal, and Sporicidal
Current edition approved Feb. 1, 2018. Published March 2018. DOI: 10.1520/
Potencies of Liquid Chemicals
E3152–18
2
E2197 Quantitative Disk Carrier Test Method for Determin-
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
ing Bactericidal, Virucidal, Fungicidal, Mycobactericidal,
Standards volume information, refer to the standard’s Document Summary page on
and Sporicidal Activities of Chemicals
the ASTM website.
3 E2471 Test Method for Using Seeded-Agar for the Screen-
The last approved version of this historical standard is referenced on
www.astm.org. ing Assessment of Antimicrobial Activity In Carpets
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E3152 − 18
E2722 Test Method for Using Seeded-Agar for the Screen- 3. Significance and Use
ing Assessment of Antimicrobial Activity in Fabric and
3.1 Fungi are known to produce objectionable odors, s
...

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Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

SIGNIFICANCE AND USE
4.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers.  
4.2 Antifungal activity is typically:  
4.2.1 Determination of article susceptibility to fungal colonization,  
4.2.2 Determination of fungistatic activity (qualitative determination of prevented or delayed fungal colonization), and  
4.2.3 Determination of fungicidal/sporicidal activity (quantitative determination of spore kill).  
4.3 The degree of required surface examination varies from gross visual examination to detailed microscopic assessment among these methods.  
4.4 This guide provides an overview of established methods and suggestions for their applicability, with consideration to the type of substrate treated or the type of antifungal treatment being assessed.
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1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This guide will encompass considerations for manufacturers regarding sources and potential causes of subvisible particles in biomanufacturing operations and the use of dynamic imaging particle analyzers as a suggested common method to monitor them. The guide will address the following components of particle analysis using dynamic imaging microscopy: fundamental principles, operation, image analysis methods, sample handling, instrument calibration, and data reporting.
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1.2 Understanding the nature of particles and their source is a key to the ability to take actions to adjust the manufacturing process to ensure final product quality. Dynamic imaging microscopy (also known as flow imaging or flow microscopy) is a useful technique for particle analysis and characterization (proteinaceous and other types) during product development, in-process and commercial release with a sensitive detection and characterization of subvisible particles at ≥2 µm and ≤100 µm (although smaller and larger particles may also be reported if data are available). In this technique brightfield illumination is used to capture images either directly in a process stream, or as a continuous sample stream passes through a flow cell positioned in the field of view of an imaging system. An algorithm performs a particle detection routine. This process is a key step during dynamic imaging. The digital particle images in the sample are processed by image morphology analysis software that quantifies the particles in size, count, image intensity, and morphological parameters. Dynamic imaging particle analyzers can produce direct determinations of the particle count per unit volume (that is, particle concentration), as a function of particle size by dividing the particle count by the volume of imaged fluid (see Appendix X1).  
1.3 This guide will describe best practices and considerations in applying dynamic imaging to identification of potential sources and causes of particles during biomanufacturing. These results can be used to monitor these particles and where possible, to adjust the manufacturing process to avoid their formation. This guide will also address the fundamental principles of dynamic imaging analysis including image analysis methods, sample preparation, instrument calibration and verification and data reporting.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
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Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

SIGNIFICANCE AND USE
4.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers.  
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5.1 The purpose of this guide is to provide guidance on characterization of the properties of porcine fibrinogen as a starting material for surgical implants and as a matrix for tissue-engineered medical products (TEMPs). This guide contains a set of physical and chemical parameters directly related to the function of porcine fibrinogen. This guide can be used to help select and characterize appropriate fibrinogen starting materials for specific purposes. Not all tests or parameters are suitable for all uses of fibrinogen.  
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1.4 The primary focus of this document is fibrinogen derived from porcine blood, which is similar to human fibrinogen. The biggest advantage that pigs have over other species (such as cattle, sheep, goats, elk, and deer) is that they are less likely to transmit transmissible spongiform encephalitis (TSE) (ISO 22442-1 Annex D; WHO Guidelines, 2003; WHO Guidelines, 2006; WHO Guidelines, 2010). The document may also discuss fibrinogen from other sources when useful information is available. Fibrin is also discussed in some sections.  
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5.1 The CFT assay provides a sensitive and reliable method to detect ricin biological activity and results can be generated within 3 h. The assay measures the amount of ricin biological activity when compared to a known ricin standard and provides a quantitative measurement for active ricin.  
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SCOPE
1.1 This specification covers preformed expansion joint filler having relatively little extrusion and substantial recovery after release from compression.  
1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.  
Note 1: Attention is called to Specifications D1752 and D994/D994M.  
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1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This specification covers hot- and cold-worked precipitation-hardenable nickel alloy rod, bar, forgings, and forging stock for high-temperature service. Chemical analysis shall be performed on the alloy and shall conform to the chemical composition requirement in carbon, manganese, silicon, phosphorus, sulfur, chromium, cobalt, molybdenum, columbium, tantalum, titanium, aluminum, zirconium, boron, iron, copper, and nickel. The material shall follow recommended annealing treatment, solution treatment, stabilizing treatment, and precipitation hardening treatment. Tension testing, hardness testing and stress-rupture testing shall be performed on the material and shall comply to the required tensile strength, yield strength, elongation, reduction in area, and Brinell hardness.
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