ASTM E3152-23

Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

SIGNIFICANCE AND USE
4.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers.  
4.2 Antifungal activity is typically:  
4.2.1 Determination of article susceptibility to fungal colonization,  
4.2.2 Determination of fungistatic activity (qualitative determination of prevented or delayed fungal colonization), and  
4.2.3 Determination of fungicidal/sporicidal activity (quantitative determination of spore kill).  
4.3 The degree of required surface examination varies from gross visual examination to detailed microscopic assessment among these methods.  
4.4 This guide provides an overview of established methods and suggestions for their applicability, with consideration to the type of substrate treated or the type of antifungal treatment being assessed.
SCOPE
1.1 This guide provides information on various test methods currently available to assess antifungal activity on natural or synthetic substrates.  
1.2 Knowledge of microbiological techniques is required for the practice of this guide.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 31-Mar-2023
• ICS: 11.100.20 - Biological evaluation of medical devices
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Refers: ASTM F1094-87(2020) - Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method
• Effective Date: 15-Apr-2020
• Refers: ASTM D6974-20 - Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• Effective Date: 01-May-2020

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3152 − 23
Standard Guide for
Standard Test Methods and Practices Available for
Determining Antifungal Activity on Natural or Synthetic
1
Substrates Treated with Antimicrobial Agents
This standard is issued under the fixed designation E3152; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D4300 Test Methods for Ability of Adhesive Films to
Support or Resist the Growth of Fungi
1.1 This guide provides information on various test methods
D4445 Test Method for Fungicides for Controlling Sapstain
currently available to assess antifungal activity on natural or
and Mold on Unseasoned Lumber (Laboratory Method)
synthetic substrates.
D4576 Test Method for Mold Growth Resistance of Wet
1.2 Knowledge of microbiological techniques is required
Blue and Wet White
for the practice of this guide.
D4783 Test Methods for Resistance of Adhesive Prepara-
1.3 This standard does not purport to address all of the
tions in Container to Attack by Bacteria, Yeast, and Fungi
safety concerns, if any, associated with its use. It is the
D5259 Test Method for Isolation and Enumeration of En-
responsibility of the user of this standard to establish appro-
terococci from Water by the Membrane Filter Procedure
priate safety, health, and environmental practices and deter-
D5590 Test Method for Determining the Resistance of Paint
mine the applicability of regulatory limitations prior to use.
Films and Related Coatings to Fungal Defacement by
1.4 This international standard was developed in accor-
Accelerated Four-Week Agar Plate Assay
dance with internationally recognized principles on standard-
D6329 Guide for Developing Methodology for Evaluating
ization established in the Decision on Principles for the
the Ability of Indoor Materials to Support Microbial
Development of International Standards, Guides and Recom-
Growth Using Static Environmental Chambers
mendations issued by the World Trade Organization Technical
D6469 Guide for Microbial Contamination in Fuels and Fuel
Barriers to Trade (TBT) Committee.
Systems
D6974 Practice for Enumeration of Viable Bacteria and
2. Referenced Documents
2 Fungi in Liquid Fuels—Filtration and Culture Procedures
2.1 ASTM Standards:
D7436 Classification System for Unfilled Polyethylene Plas-
C1338 Test Method for Determining Fungi Resistance of
tics Molding and Extrusion Materials with a Fractional
Insulation Materials and Facings
Melt Index Using ISO Protocol and Methodology
D2020 Test Methods for Mildew (Fungus) Resistance of
3
D7584 Test Method for Evaluating the Resistance of the
Paper and Paperboard (Withdrawn 2009)
Surface of Wet Blue and Wet White to the Growth of
D3273 Test Method for Resistance to Growth of Mold on the
Fungi in an Environmental Chamber
Surface of Interior Coatings in an Environmental Cham-
ber D7855/D7855M Test Method for Determination of Mold
D3456 Practice for Determining by Exterior Exposure Tests Growth on Coated Building Products Designed for Inte-
the Susceptibility of Paint Films to Microbiological Attack
rior Applications Using an Environmental Chamber and
Indirect Inoculation
D7910 Practice for Collection of Fungal Material From
1
This guide is under the jurisdiction of ASTM Committee E35 on Pesticides,
Surfaces by Tape Lift
Antimicrobials, and Alternative Control Agents and is the direct responsibility of
E1326 Guide for Evaluating Non-culture Microbiological
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2023. Published April 2023. Originally
Tests
approved in 2018. Last previous edition approved in 2018 as E3152 – 18. DOI:
E2111 Quantitative Carrier Test Method to Evaluate the
10.1520/E3152-23.
2 Bactericidal, Fungicidal, Mycobactericidal, and Sporicidal
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Potencies of Liquid Chemicals
Standards volume information, refer to the standard’s Document Summary page on
E2197 Quantitative Disk Carrier Test Method for Determin-
the ASTM website.
3 ing Bactericidal, Virucidal, Fungicidal, Mycobactericidal,
The last approved version of this historical standard is referenced on
www.astm.org. and Sporicidal Activities of Chemicals
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E3152 − 23
E2471 Test
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E3152 − 18 E3152 − 23
Standard Guide for
Standard Test Methods and Practices Available for
Determining Antifungal Activity on Natural or Synthetic
1
Substrates Treated with Antimicrobial Agents
This standard is issued under the fixed designation E3152; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This guide provides information on various test methods currently available to assess antifungal activity on natural or synthetic
substrates.
1.2 Knowledge of microbiological techniques is required for the practice of this guide.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of
regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
C1338 Test Method for Determining Fungi Resistance of Insulation Materials and Facings
3
D2020 Test Methods for Mildew (Fungus) Resistance of Paper and Paperboard (Withdrawn 2009)
D3273 Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber
D3456 Practice for Determining by Exterior Exposure Tests the Susceptibility of Paint Films to Microbiological Attack
D4141/D4141M Practice for Conducting Black Box and Solar Concentrating Exposures of Coatings
D4300 Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi
D4445 Test Method for Fungicides for Controlling Sapstain and Mold on Unseasoned Lumber (Laboratory Method)
D4576 Test Method for Mold Growth Resistance of Wet Blue and Wet White
D4783 Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi
D5259 Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
D5590 Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated
Four-Week Agar Plate Assay
1
This guide is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Feb. 1, 2018April 1, 2023. Published March 2018April 2023. Originally approved in 2018. Last previous edition approved in 2018 as E3152 – 18.
DOI: 10.1520/E3152–1810.1520/E3152-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
The last approved version of this historical standard is referenced on www.astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E3152 − 23
D6329 Guide for Developing Methodology for Evaluating the Ability of Indoor Materials to Support Microbial Growth Using
Static Environmental Chambers
D6469 Guide for Microbial Contamination in Fuels and Fuel Systems
D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
D7436 Classification System for Unfilled Polyethylene Plastics Molding and Extrusion Materials with a Fractional Melt Index
Using ISO Protocol and Methodology
D7584 Test Method for Evaluating the Resistance of the Surface of Wet Blue and Wet White to the Growth of Fungi in an
Environmental Chamber
D7855/D7855M Test Method for Determination of Mold Growth on Coated Building Products Designed for Interior
Applications Using an Environmental Chamber and Indirect Inoculation
D7910 Practice for Collection of Fungal Material From Surfaces by Tape Lift
E1326 Guide for Evaluating Non-culture Microbiological Tests
E2111 Quantitative Carrier Test Method to Evaluate the Bactericidal
...