ASTM E2911-13

Standard Guide for Relative Intensity Correction of Raman Spectrometers (Withdrawn 2022)

SIGNIFICANCE AND USE
4.1 Generally, Raman spectra measured using grating-based dispersive or Fourier transform Raman spectrometers have not been corrected for the instrumental response (spectral responsivity of the detection system). Raman spectra obtained with different instruments may show significant variations in the measured relative peak intensities of a sample compound. This is mainly as a result of differences in their wavelength-dependent optical transmission and detector efficiencies. These variations can be particularly large when widely different laser excitation wavelengths are used, but can occur when the same laser excitation is used and spectra of the same compound are compared between instruments. This is illustrated in Fig. 1, which shows the uncorrected luminescence spectrum of SRM 2241, acquired upon four different commercially available Raman spectrometers operating with 785 nm laser excitation. Instrumental response variations can also occur on the same instrument after a component change or service work has been performed. Each spectrometer, due to its unique combination of filters, grating, collection optics and detector response, has a very unique spectral response. The spectrometer dependent spectral response will of course also affect the shape of Raman spectra acquired upon these systems. The shape of this response is not to be construed as either “good or bad” but is the result of design considerations by the spectrometer manufacturer. For instance, as shown in Fig. 1, spectral coverage can vary considerably between spectrometer systems. This is typically a deliberate tradeoff in spectrometer design, where spectral coverage is sacrificed for enhanced spectral resolution.
SCOPE
1.1 This guide is designed to enable the user to correct a Raman spectrometer for its relative spectral-intensity response function using NIST Standard Reference Materials2 in the 224X series (currently SRMs 2241, 2242, 2243, 2244, 2245, 2246), or a calibrated irradiance source. This relative intensity correction procedure will enable the intercomparison of Raman spectra acquired from differing instruments, excitation wavelengths, and laboratories.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 Because of the significant dangers associated with the use of lasers, ANSI Z136.1 or suitable regional standards should be followed in conjunction with this practice.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Jun-2013
• ICS: 71.040.50 - Physicochemical methods of analysis
• Technical Committee: E13 - Molecular Spectroscopy and Separation Science
• Drafting Committee: E13.08 - Raman Spectroscopy

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2911 − 13
Standard Guide for
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Relative Intensity Correction of Raman Spectrometers
This standard is issued under the fixed designation E2911; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
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1. Scope 2.2 ANSI Standard:
Z136.1Safe Use of Lasers
1.1 This guide is designed to enable the user to correct a
Raman spectrometer for its relative spectral-intensity response
3. Terminology
2
function using NIST Standard Reference Materials in the
3.1 Definitions—Terminology used in this practice con-
224X series (currently SRMs 2241, 2242, 2243, 2244, 2245,
forms to the definitions in Terminology E131.
2246), or a calibrated irradiance source. This relative intensity
4. Significance and Use
correctionprocedurewillenabletheintercomparisonofRaman
spectra acquired from differing instruments, excitation
4.1 Generally,Ramanspectrameasuredusinggrating-based
wavelengths, and laboratories.
dispersive or Fourier transform Raman spectrometers have not
been corrected for the instrumental response (spectral respon-
1.2 The values stated in SI units are to be regarded as
sivity of the detection system). Raman spectra obtained with
standard. No other units of measurement are included in this
different instruments may show significant variations in the
standard.
measured relative peak intensities of a sample compound.This
1.3 Because of the significant dangers associated with the
is mainly as a result of differences in their wavelength-
use of lasers, ANSI Z136.1 or suitable regional standards
dependent optical transmission and detector efficiencies.These
should be followed in conjunction with this practice.
variations can be particularly large when widely different laser
1.4 This standard does not purport to address all of the
excitation wavelengths are used, but can occur when the same
safety concerns, if any, associated with its use. It is the
laser excitation is used and spectra of the same compound are
responsibility of the user of this standard to establish appro-
compared between instruments. This is illustrated in Fig. 1,
priate safety and health practices and determine the applica-
which shows the uncorrected luminescence spectrum of SRM
bility of regulatory limitations prior to use.
2241, acquired upon four different commercially available
Raman spectrometers operating with 785 nm laser excitation.
2. Referenced Documents
Instrumental response variations can also occur on the same
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instrument after a component change or service work has been
2.1 ASTM Standards:
E131Terminology Relating to Molecular Spectroscopy performed. Each spectrometer, due to its unique combination
offilters,grating,collectionopticsanddetectorresponse,hasa
E1840Guide for Raman Shift Standards for Spectrometer
Calibration very unique spectral response. The spectrometer dependent
spectral response will of course also affect the shape of Raman
E2529Guide for Testing the Resolution of a Raman Spec-
trometer spectra acquired upon these systems. The shape of this re-
sponse is not to be construed as either “good or bad” but is the
result of design considerations by the spectrometer manufac-
1 turer. For instance, as shown in Fig. 1, spectral coverage can
This guide is under the jurisdiction of ASTM Committee E13 on Molecular
Spectroscopy and Separation Science and is the direct responsibility of Subcom- vary considerably between spectrometer systems. This is
mittee E13.08 on Raman Spectroscopy.
typically a deliberate tradeoff in spectrometer design, where
Current edition approved July 1, 2013. Published July 2013. DOI: 10.1520/
spectralcoverageissacrificedforenhancedspectralresolution.
E2911–13
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Trademark of and available from NIST Office of Reference Materials, 100
4.2 Variations in spectral peak intensities can be mostly
Bureau Drive, Stop 2300, Gaithersburg, MD 20899-2300. http://www.nist.gov/srm.
corrected through calibration of the Raman intensity (y) axis.
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
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Standards volume information, refer to the standard’s Document Summary page on Available fromAmerican National Standards Institute (ANSI), 25 W. 43rd St.,
the ASTM website. 4th Floor, New York, NY 10036, http://www.ansi.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E2911 − 13
FIG. 1 SRM 2241 Measured on Four Commercial Raman Spectrometers Utilizing 785 nm Excitation
The conventional method of calibrati
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