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ASTM E1657-98(2001)

(Practice)

Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography

Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography

SCOPE
1.1 This practice is intended to serve as a guide for the testing of the performance of a variable-wavelength photometric detector (VWPD) used as the detection component of a liquid-chromatographic (LC) system operating at one or more wavelengths in the range 190 to 800 nm. Many of the measurements are made at 254 nm for consistency with Practice E685. Measurements at other wavelengths are optional.
1.2 This practice is intended to describe the performance of the detector both independently of the chromatographic system (static conditions) and with flowing solvent (dynamic conditions).
1.3 For general liquid chromatographic procedures, consult Refs (1-9).
1.4 For general information concerning the principles, construction, operation, and evaluation of liquid-chromatography detectors, see Refs (10,11 )in addition to the sections devoted to detectors in Refs  (1-7).
1.5 The values stated in SI units are to be regarded as standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
31-Dec-2000
ICS
71.040.50 - Physicochemical methods of analysis
Technical Committee
E13 - Molecular Spectroscopy and Separation Science
Drafting Committee
E13.19 - Separation Science
Current Stage
Ref Project

Relations

Replaces
ASTM E1657-98 - Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography
Effective Date
01-Jan-2001
Replaced By
ASTM E1657-98(2006) - Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography
Effective Date
01-Mar-2006
Referred By
ASTM D6953-18 - Standard Test Method for Determination of Antioxidants and Erucamide Slip Additives in Polyethylene Using Liquid Chromatography (LC)
Effective Date
01-Jan-2001

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ASTM E1657-98(2001) - Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid ChromatographyASTM E1657-98(2001) - Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography
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ASTM E1657-98(2001) - Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn. Contact ASTM
International (www.astm.org) for the latest information.
Designation:E1657–98(Reapproved2001)
Standard Practice for
Testing Variable-Wavelength Photometric Detectors Used in
1
Liquid Chromatography
This standard is issued under the fixed designation E 1657; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope E 685 Practice for Testing Fixed-Wavelength Photometric
4
Detectors Used in Liquid Chromatography
1.1 This practice is intended to serve as a guide for the
testing of the performance of a variable-wavelength photomet-
3. Terminology
ric detector (VWPD) used as the detection component of a
3.1 Definitions:
liquid-chromatographic (LC) system operating at one or more
3.1.1 absorbance calibration—the procedure that verifies
wavelengths in the range 190 to 800 nm. Many of the
that the absorbance scale is correct within 65%.
measurements are made at 254 nm for consistency with
3.1.2 drift—the average slope of the noise envelope ex-
Practice E 685. Measurements at other wavelengths are op-
pressed in absorbance units per hour (AU/h) as measured over
tional.
a period of 1 h.
1.2 This practice is intended to describe the performance of
3.1.3 dynamic—under conditions of a flow rate of 1.0
the detector both independently of the chromatographic system
mL/min.
(static conditions) and with flowing solvent (dynamic condi-
3.1.4 linear range—of a VWPD,therangeofconcentrations
tions).
of a test substance in a test solvent over which the ratio of
1.3 For general liquid chromatographic procedures, consult
2 response of the detector versus concentration of test substance
Refs (1-9).
is constant to within 5 % as determined from the linearity plot
1.4 For general information concerning the principles, con-
specified in 7.1.2 and illustrated in Fig. 1. The linear range
struction, operation, and evaluation of liquid-chromatography
should be expressed as the ratio of the upper limit of linearity
detectors, see Refs (10, 11) in addition to the sections devoted
obtained from the plot to either a) the lower linear concentra-
to detectors in Refs (1-7).
tion, or b) the minimum detectable concentration, if the
1.5 The values stated in SI units are to be regarded as
minimum detectable concentration is greater than the lower
standard.
linear concentration.
1.6 This standard does not purport to address all of the
3.1.5 long-term noise—the maximum amplitude in AU for
safety concerns, if any, associated with its use. It is the
all random variations of the detector signal of frequencies
responsibility of the user of this standard to establish appro-
between 6 and 60 cycles per hour (0.1 and 1.0 cycles per min).
priate safety and health practices and determine the applica-
3.1.5.1 Discussion—Itrepresentsnoisethatcanbemistaken
bility of regulatory limitations prior to use.
for a late-eluting peak. This noise corresponds to the observed
2. Referenced Documents noise only and may not always be present.
3.1.6 minimum detectability— of a VWPD, that concentra-
2.1 ASTM Standards:
tion of a specific solute in a specific solvent that results in a
E 275 Practice for Describing and Measuring Performance
detector response corresponding to twice the static short-term
of Ultraviolet, Visible, and Near-Infrared Spectrophotom-
3 noise.
eters
3.1.6.1 Discussion—The static short-term noise is a mea-
E 682 Practice for Liquid Chromatography Terms and Re-
4 surement of peak-to-peak noise.Astatistical approach to noise
lationships
suggests that a value of three times the rms (root-mean-square)
noise would insure that any value outside this range would not
1
This practice is under the jurisdiction of ASTM Committee E13 on Molecular
be noise with a confidence level of greater than 99 %. Since
Spectroscopy and is the direct responsibility of Subcommittee E13.19 on Chroma-
peak-to-peak noise is approximately five times the rms noise
tography.
(12), the minimum detectability defined in this practice is a
Current edition approved Nov. 10, 1998. Published January 1999. Originally
more conservative estimate.
published as E 1657 – 94. Last previous edition E 1657 – 96.
2
The boldface numbers in parentheses refer to the list of references at the end of
3.1.7 response time (speed of output)— the detector, the
this practice.
time required for the detector output to change from 10 % to
3
Annual Book of ASTM Standards, Vol 03.06.
4
90 % of the new equilibrium value when the composition of
Annual Book of ASTM Standards, Vol 14.02.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

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NOTICE: This standa
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