ASTM D2269-99(2005)
(Test Method)Standard Test Method for Evaluation of White Mineral Oils by Ultraviolet Absorption
Standard Test Method for Evaluation of White Mineral Oils by Ultraviolet Absorption
SIGNIFICANCE AND USE
The ultraviolet absorption of white mineral oils is used to determine their suitability for use in food, drug, and cosmetic applications.
The U.S. Pharmacopeia and the National Formulary specifications for mineral oil require the measurement of ultraviolet absorption.
SCOPE
1.1 This test method describes a procedure for the examination and evaluation of NF and USP grade white mineral oils.
1.2 This test method is not applicable to oils containing additives soluble in dimethyl sulfoxide (DMSO) that exhibit fluorescence or fluorescence quenching properties.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific warning statement, see 7.1.1-7.1.3
General Information
Relations
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation:D2269–99 (Reapproved 2005)
Standard Test Method for
Evaluation of White Mineral Oils by Ultraviolet Absorption
This standard is issued under the fixed designation D2269; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 radiant energy, n—energy transmitted as electromag-
netic waves.
1.1 This test method describes a procedure for the exami-
3.1.3 radiant power, P, n—the rate at which energy is
nation and evaluation of NF and USPgrade white mineral oils.
transported in a beam of radiant energy.
1.2 This test method is not applicable to oils containing
3.2 Definitions of Terms Specific to This Standard:
additives soluble in dimethyl sulfoxide (DMSO) that exhibit
3.2.1 absorbance, A, n—the logarithm to the base 10 of the
fluorescence or fluorescence quenching properties.
reciprocal of the transmittance, T. In symbols:
1.3 The values stated in SI units are to be regarded as
standard. No other units of measurement are included in this A 5 log ~1/T!52log T
10 10
standard.
where T is the transmittance as defined in 3.2.5.
1.4 This standard does not purport to address all of the
3.2.2 absorptivity, a, n—the absorbance divided by the
safety concerns, if any, associated with its use. It is the
product of sample pathlength and concentration. In symbols:
responsibility of the user of this standard to establish appro-
a 5 A/bc
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use. For specific
whereAistheabsorbanceasdefinedin3.2.1,bisthesample
warning statements, see 7.1.1-7.1.3.
pathlength as defined in 3.2.4, and c is the concentration as
2. Referenced Documents
defined in 3.2.3.
3.2.3 concentration, c, n—the quantity of sample expressed
2.1 ASTM Standards:
in grams per litre.
D1840 TestMethodforNaphthaleneHydrocarbonsinAvia-
3.2.4 sample pathlength, b, n—the distance in centimetres,
tion Turbine Fuels by Ultraviolet Spectrophotometry
measuredinthedirectionofpropagationofthebeamofradiant
E131 Terminology Relating to Molecular Spectroscopy
energy, between the surfaces of the specimen on which the
E275 Practice for Describing and Measuring Performance
radiant energy is incident and the surface of the specimen from
of Ultraviolet and Visible Spectrophotometers
which it is emergent.
2.2 Other Standard:
3.2.5 transmittance, T, n—the ratio of the radiant power
U.S. Pharmacopeia USP XIII/National Formulary (NF
transmitted by the mineral oil extract in its cell to the radiant
XVIII)
power transmitted by the solvent control in its cell. Expressed
3. Terminology
by:
3.1 Definitions:
T 5 P /P
e c
3.1.1 For definitions of terms and symbols relating to
where P is the radiant power transmitted by the mineral oil
e
absorption spectroscopy see Terminology E131. Terms of
extract and P is the radiant power transmitted by the solvent
c
particular significance are the following:
control.
4. Summary of Test Method
This test method is under the jurisdiction of ASTM Committee D02 on
Petroleum Products and Lubricants and is the direct responsibility of Subcommittee 4.1 Asample of oil is extracted with dimethyl sulfoxide and
D02.04 on Hydrocarbon Analysis.
the ultraviolet absorbance of the extract is determined in the
Current edition approved June 1, 2005. Published August 2005. Originally
range from 260 to 350 nm. The absorbance is compared with
´1
approved in 1964. Last previous edition approved in 1999 as D2269–99 . DOI:
that of a naphthalene standard.
10.1520/D2269-99R05.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
Available from The United States Pharmacopeia (USP), 12601 Twinbrook
Parkway, Rockville, MD 20852.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
D2269–99 (2005)
charcoal. Thecolumnis25mmindiameter,drawnto6.4mmindiameter
5. Significance and Use
at the bottom and has a reservoir at the top for containing the liquid. Glass
5.1 The ultraviolet absorption of white mineral oils is used
wool is placed in the bottom of the column, and about 13 mm of 0.707 to
to determine their suitability for use in food, drug, and
0.074 (25 to 200 mesh) or 0.0149 to 0.074 (100 to 200 mesh) silica gel is
cosmetic applications.
placed on top of it. The column is filled with activated charcoal and the
5.2 The U.S. Pharmacopeia and the National Formulary spectroscopic solvent poured into the reservoir and allowed to percolate
through the charcoal at atmospheric pressure. The purified solvent is
specifications for mineral oil require the measurement of
collected at the bottom of the column and stored in glass-stoppered bottles
ultraviolet absorption.
as it is very hygroscopic and reacts with some metal containers in the
presence of air.
6. Apparatus
6.1 Spectrophotometer, equipped to handle liquid samples 7.2 Naphthalene—high-purity, 99+ %.
in 1-cm path length cells. The instrument shall be capable of 7.3 Standard Reference Solution—Asolution containing 7.0
measuring absorbance with a repeatability of 61.0 % or better mg of naphthalene per litre of purified isooctane.
from an average at the 0.4 absorbance level in the spectral 7.4 Standard Reference Spectrum—The absorbance curve
region near 290 nm with a nominal band width of 1 nm or less. obtained by scanning the standard reference solution in the
range from 260 to 350 nm against isooctane of the same
NOTE 1—For recommended methods of testing spectrophotometers to
spectral purity as that used to prepare the standard (7.1.2).
be used in this test method refer to Practice E275.
7.5 Standard Reference Absorbance— The absorbance at
6.2 Fused Quartz Cells, two, having path lengths of 1.00 6
275 nm of the standard reference spectrum.
0.005 cm, or better. The distance in centimetres does not
NOTE 4—This absorbance will be approximately 0.30.
include the thickness of the cell in which the sample is
contained.
8. Procedure
6.3 Separatory Funnels, glass-stoppered, 125-mL capacity,
8.1 Transfer 25 mL of the mineral oil and 25 mL of hexane
equipped with TFE-fluorocarbon stopcocks or other suitable
to a separatory funnel and mix. Add 5.0 mL of dimethyl
stopcocks that will not contaminate the solvents used.
sulfoxide and shake the mixture vigorously for at least 1 min.
7. Reagents and Materials
Allow to stand until the lower layer is clear.
7.1 Solvents: 8.2 Transfer the lower layer to a separatory funnel, add 2
mLofhexaneandshakethem
...
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