ASTM E653-91(2003)

Standard Test Method for Effectiveness of Aerosol and Pressurized Space Spray Insecticides Against Flying Insects

SIGNIFICANCE AND USE
This test method provides a satisfactory means of determining the relative efficacy of aerosol and pressurized space spray insecticide formulations against house flies (Musca domestica, L) strains.
Test data obtained by this test method may also be adequate to support label claims for the use of the product against mosquitoes, gnats, flying moths, wasps, and certain other small flying insects. This test method is not designed to measure the residual activity.
As a biological test, it is subject to the variations that accompany the reaction of living organisms. It should be employed under the supervision of personnel familiar with the biological testing of insecticides.
SCOPE
1.1 This method determines the effectiveness of aerosol and pressurized space-spray insecticides against house flies ( Musca domestica L ) and, with modifications in dosage, other flying insects.  
1.2 The test may be conducted using approximately 100 house flies per test (small group) or 500 flies per test (large group).  
1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 14-Jul-1991
• ICS: 71.100.99 - Other products of the chemical industry
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Relations

• Replaces: ASTM E653-91(1996)e1 - Standard Method for Testing Effectiveness of Aerosol and Pressurized Space Spray Insecticides Against Flying Insects
• Effective Date: 15-Jul-1991
• Replaced By: ASTM E653-91(2009) - Standard Test Method for Effectiveness of Aerosol and Pressurized Space Spray Insecticides Against Flying Insects (Withdrawn 2018)
• Effective Date: 01-Apr-2009

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E653–91(Reapproved 2003)
Standard Test Method for
Effectiveness of Aerosol and Pressurized Space Spray
1
Insecticides Against Flying Insects
This standard is issued under the fixed designation E 653; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.4 pressurized sprays—these products deliver mist
sprays intermediate between aerosols and sprays intended to
1.1 This test method determines the effectiveness of aerosol
deposit an insecticidal residue. They produce sprays in which
and pressurized space-spray insecticides against house flies
less than 80 % of the particles have an arithmetic mean
(Musca domestica L) and, with modifications in dosage, other
diameter of 30 µm and many are 50 µm to 100 µm in mean
flying insects.
diameter. Pressurized sprays shall be no less effective than the
1.2 The test may be conducted using approximately 100
selected reference standards when tested against house flies at
house flies per test (small group) or 500 flies per test (large
no more than twice the dosage specified for the selected
group).
reference standard.
1.3 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
4. Summary of Test Method
responsibility of the user of this standard to establish appro-
4.1 If the small-group method is used, ten tests are run on
priate safety and health practices and determine the applica-
the Official Test Aerosol (using the selected reference stan-
bility of regulatory limitations prior to use.
3
dard) and on each of the specimens in parallel.The specimens
2. Referenced Document of a series shall be randomized in the order of testing.
2
4.2 If the large-group method is used, the test is conducted
2.1 ASTM Standards:
as in 4.1, with the exception that five, rather than ten tests are
E 652 Test Method for Nonresidual Liquid Household In-
required.
secticides Against Flying Insects
4.3 The average percentage mortality of the test insecticide
3. Terminology
compared with that of the selected reference standard is the
basisforassigningeitherGradeA(aerosolorpressurizedspace
3.1 Definitions of Terms Specific to This Standard:
spray) or Grade B (pressurized space spray) rating to the test
3.1.1 aerosols—for this test method, the spray from aerosol
specimen.
dispensers should be in finely divided form in which 80 % or
more of the individual spray particles have an arithmetic mean
5. Significance and Use
diameter of 30 µm or less, and none of the spray particles have
5.1 This test method provides a satisfactory means of
a diameter of more than 50 µm. Aerosols shall be no less
determining the relative efficacy of aerosol and pressurized
effective than the selected reference standards when tested
spacesprayinsecticideformulationsagainsthouseflies(Musca
against house flies at the same dosage or less.
domestica, L) strains.
3.1.2 fly culture—all adults resulting from the seeding of
5.2 Test data obtained by this test method may also be
eggs collected at one time on a given date.
adequate to support label claims for the use of the product
3.1.3 knocked-down flies—all adult test flies incapable of
against mosquitoes, gnats, flying moths, wasps, and certain
coordinated movement (moribund).
other small flying insects. This test method is not designed to
measure the residual activity.
1
This test method is under the jurisdiction of ASTM Committee E35 on
5.3 As a biological test, it is subject to the variations that
Pesticides and is the direct responsibility of Subcommittee E35.12 on Insect Control
accompany the reaction of living organisms. It should be
Agents. It was originally developed by the Chemical Specialties Manufacturers
employed under the supervision of personnel familiar with the
Association (CSMA).
Current edition approved July 15, 1991. Published September 1991. Originally
biological testing of insecticides.
approved in 1978. Last previous edition approved in 1984 as E 653–84.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM The Official Test Aerosol (Selected Reference Standard) has been found
Standards volume information, refer to the standard’s Document Summary page on suitable for this test and is available from CSMA, 1913 Eye Street N.W.,
the ASTM website. Washington, DC 20006.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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E653–91 (2003)
6. Apparatus 6.9 Apparatus for Collecting Treat
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