FprEN ISO 25202

• Abstract:

  This international standard specifies a method for the determination of the content of Leather.; This method is based on high performance liquid chromatography (HPLC). It is selective and not sensitive to coloured extracts.

• Status: Not Published
• Publication Date: 01-Nov-2026
• ICS: 59.140.30 - Leather and furs
• Technical Committee: CEN/TC 289 - Leather
• Drafting Committee: CEN/TC 289/WG 1 - Chemical test methods
• Current Stage: 5020 - Submission to Vote - Formal Approval
• Start Date: 16-Jul-2026
• Completion Date: 16-Jul-2026
• Ref Project: oSIST prEN ISO 25202:2025 - Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS 25202:2024)

• Consolidated By: ISO/FDIS 25202 - Leather — Chemical analysis — Determination of glutaraldehyde content
• Effective Date: 12-Feb-2026

Overview

FprEN ISO 25202: Leather - Chemical analysis - Determination of glutaraldehyde content is an international standard developed by CEN and ISO. The standard outlines a reliable and selective method for the quantitative determination of free and released glutaraldehyde in leather using high performance liquid chromatography (HPLC). This method ensures accurate detection of glutaraldehyde, a common chemical used in leather processing, without interference from colored extracts-a frequent challenge in the analysis of treated leather materials.

The standard supports quality control, safety compliance, and regulatory requirements within the leather industry, fostering international consistency in testing procedures and results.

Key Topics

• Purpose: Establishes a standardized procedure for determining the content of glutaraldehyde in leather materials, ensuring product safety and regulatory transparency.
• Method Principle:
  • Utilizes high performance liquid chromatography (HPLC/Liquid Chromatography) with UV detection.
  • Separates and quantifies glutaraldehyde derivatives, even in the presence of other aldehydes and ketones.
  • Extraction involves sample elution with detergent, chemical reaction with 2,4-dinitrophenylhydrazine (DNPH), and subsequent measurement using LC.
• Sample Preparation:
  • Specific procedures for preparing specimens, including sampling according to established ISO standards for leather.
  • Extraction parameters tailored to optimize glutaraldehyde release and detection.
• Selectivity:
  • Method is not sensitive to color, minimizing interference from dyed leathers and colored extracts.
• Reporting:
  • Results are reported as glutaraldehyde content in mg/kg.
  • Requires detailed documentation including sample identification, testing conditions, and deviations from procedure.

Applications

FprEN ISO 25202 is vital in several practical scenarios within the leather sector:

• Leather Manufacturing: Ensures precise monitoring of residual glutaraldehyde, supporting both process optimization and compliance with safety standards.
• Product Testing and Certification: Enables manufacturers and laboratories to provide verifiable data for regulatory agencies and customers regarding chemical content in finished leather products, including shoes, garments, furniture, and automotive components.
• Environmental & Consumer Safety: Supports risk assessments related to skin contact and environmental exposure by verifying the levels of potentially hazardous substances.
• Import/Export and Regulatory Compliance: Facilitates international trade of leather goods by complying with recognized chemical safety and labeling requirements.

Related Standards

To ensure consistency and reliability in chemical analysis of leather, FprEN ISO 25202 references and complements several related standards:

• ISO 2418: Leather - Chemical, physical, mechanical, and fastness tests - Position and preparation of specimens for testing
• ISO 4044: Leather - Chemical tests - Preparation of chemical test samples
• ISO 3696: Water for analytical laboratory use - Specification and test methods
• ISO 17226-1: Leather - Chemical determination of formaldehyde content - Part 1: Method using high-performance liquid chromatography

These associated standards provide guidance for specimen preparation, testing protocols, and use of specified analytical reagents, supporting accurate and replicable results across the leather industry.

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Keywords: FprEN ISO 25202, leather chemical analysis, glutaraldehyde determination, high performance liquid chromatography, HPLC, leather testing standards, CEN, ISO, DNPH, quality control, regulatory compliance, leather manufacturing, chemical safety.

SLOVENSKI STANDARD
01-februar-2025
Usnje - Kemijska analiza - Določevanje vsebnosti glutaraldehida (ISO/DIS
25202:2024)
Leather - Chemical analysis - Determination of glutaraldehyde content (ISO/DIS
25202:2024)
Leder - Chemische Analyse - Bestimmung des Glutaraldehydgehalts (ISO/DIS
25202:2024)
Cuir - Analyse chimique - Détermination de la teneur en glutaraldéhyde (ISO/DIS
25202:2024)
Ta slovenski standard je istoveten z: prEN ISO 25202
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.40 Kemijska analiza Chemical analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
2024-12-11
ICS: 59.140.30
Voting terminates on:
2025-03-05
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference numbers
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
DRAFT
ISO/DIS 25202:2024(en)
International
Standard
ISO/DIS 25202
IULTCS
IUC 40
IULTCS
Leather — Chemical analysis —
Secretariat: ISO
Determination of glutaraldehyde
content
Voting begins on:
ICS: 59.140.30
Voting terminates on:
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: [phone]
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: [e-mail]
Website: www.iso.org
Reference numbers
Published in Switzerland ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
ii
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Preparation of test specimens . 2
8 Procedure . 3
8.1 Sample processing .3
8.1.1 Extraction .3
8.1.2 Reaction with DNPH .3
8.2 Determination by LC .3
9 Expression of results . 4
9.1 General .4
9.2 Calculation of the glutaraldehyde content .4
9.3 Precision of the test method .4
10 Test report . 4
Annex A (informative) LC conditions . 5
Bibliography . 6

iii
ISO/DIS 25202:2024(en)
IULTCS/IUC 40:2024(en)
Foreword
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in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
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patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of
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For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
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Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www.iso.org/iso/foreword.html.
This document was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 289, Leather, in collaboration with the Chemical Test Commission of the International Union of
Leather Technologists and Chemists Societies (IUC Commission, IULTCS), in accordance with the Agreement
on technical cooperation between ISO and CEN (Vienna Agreement). This method is technically similar to
the Colorimetric Section of the method IUC 19 which was declared an official method at the IULTCS Delegates
meeting on 31st May 2003 i
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