EN ISO 22160:2007

Milk and milk-based drinks - Determination of alkaline phosphatase activity - Enzymatic photo-activated system (EPAS) method (ISO 22160:2007)

ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method.
The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats.
The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.

Milch und flüssige Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase - Verfahren mit einem enzymatisch photoaktivierten System (EPAS) (ISO 22160:2007)

Diese Internationale Norm legt ein Verfahren für die Bestimmung der Aktivität der alkalischen Phosphatase in pasteurisierter Vollmilch, Halbfettmilch, Magermilch, Sahne und aromatisierten Milcherzeugnissen mit einem chemilumineszenten (EPAS) Verfahren fest.
Das Verfahren ist für Milch und flüssige Milcherzeugnisse von Kühen, Schafen, Büffeln und Ziegen anwendbar.
Dieses Verfahren ist auch auf Flüssigproben anwendbar, die so verdünnt werden, dass die Aktivität der alkalischen Phosphatase weniger als 7 000 Milliunits je Liter (mU/l) beträgt.
ANMERKUNG   Ein erfolgreicher Ringversuch fand statt, sowohl mit Vollmilch von Kühen, Schafen, Büffeln und Ziegen als auch mit Magermilch von Kühen (Fettgehalt < 0,5 %), Sahne mit 20 % Fettgehalt und Schokoladenmilch mit 2 % Fettgehalt (jeweils Massenanteil).

Lait et boissons à base de lait - Détermination de l'activité de la phosphatase alcaline - Méthode par un système de photoactivation enzymatique (ISO 22160:2007)

L'ISO 22160|FIL 209:2007 spécifie une méthode de détermination de l'activité de la phosphatase alcaline dans les laits pasteurisés entier, demi écrémé, écrémé, la crème et les laits aromatisés, en utilisant une méthode chimiluminescente par photoactivation enzymatique (EPAS).
La présente méthode est applicable au lait de vache, de brebis, de bufflonne et de chèvre ainsi qu'aux boissons à base de lait de ces espèces.
Cette méthode convient également pour tout échantillon liquide, dilué de sorte que l'activité de la phosphatase alcaline diluée présente une teneur inférieure à 7 000 milliunités par litre.

Mleko in pijače na osnovi mleka – Določevanje aktivnosti alkalne fosfataze - Metoda EPAS (Enzymatic photo-activated system) (ISO 22160:2007)

General Information

• Status: Published
• Publication Date: 31-Mar-2007
• Withdrawal Date: 30-Oct-2007
• ICS: 67.100.10 - Milk and processed milk products
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 01-Apr-2007
• Completion Date: 01-Apr-2007
• Ref Project: SIST EN ISO 22160:2007 - Milk and milk-based drinks - Determination of alkaline phosphatase activity - Enzymatic photo-activated system (EPAS) method (ISO 22160:2007)

Relations

• Replaces: EN ISO 11816-1:2000 - Milk and milk products - Determination of alkaline phosphatase activity using a fluorimetric method - Part 1: Milk and milk-based drinks (ISO 11816-1:1997)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
01-julij-2007
0OHNRLQSLMDþHQDRVQRYLPOHND±'RORþHYDQMHDNWLYQRVWLDONDOQHIRVIDWD]H
0HWRGD(3$6(Q]\PDWLFSKRWRDFWLYDWHGV\VWHP,62
Milk and milk-based drinks - Determination of alkaline phosphatase activity - Enzymatic
photo-activated system (EPAS) method (ISO 22160:2007)
Milch und flüssige Milcherzeugnisse - Bestimmung der Aktivität der alkalischen
Phosphatase - Verfahren mit einem enzymatisch photoaktivierten System (EPAS) (ISO
22160:2007)
Lait et boissons a base de lait - Détermination de l'activité de la phosphatase alcaline -
Méthode par un systeme de photoactivation enzymatique (ISO 22160:2007)
Ta slovenski standard je istoveten z: EN ISO 22160:2007
ICS:
67.100.10 0OHNRLQSUHGHODQLPOHþQL Milk and processed milk
SURL]YRGL products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 22160
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2007
ICS 67.100.10 Supersedes EN ISO 11816-1:2000
English Version
Milk and milk-based drinks - Determination of alkaline
phosphatase activity - Enzymatic photo-activated system
(EPAS) method (ISO 22160:2007)
Lait et boissons à base de lait - Détermination de l'activité Milch und flüssige Milcherzeugnisse - Bestimmung der
de la phosphatase alcaline - Méthode par un système de Aktivität der alkalischen Phosphatase - Verfahren mit
photoactivation enzymatique (ISO 22160:2007) einem enzymatisch photoaktivierten System (EPAS) (ISO
22160:2007)
This European Standard was approved by CEN on 6 March 2007.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22160:2007: E
worldwide for CEN national Members.

Foreword
This document (EN ISO 22160:2007) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and
milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by October 2007, and conflicting national
standards shall be withdrawn at the latest by October 2007.

This document supersedes EN ISO 11816-1:2000.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.
Endorsement notice
The text of ISO 22160:2007 has been approved by CEN as EN ISO 22160:2007 without any
modifications.
INTERNATIONAL ISO
STANDARD 22160
IDF
First edition
2007-04-01
Milk and milk-based drinks —
Determination of alkaline phosphatase
activity — Enzymatic photo-activated
system (EPAS) method
Lait et boissons à base de lait — Détermination de l'activité de la
phosphatase alcaline — Méthode par un système de photoactivation
enzymatique
Reference numbers
ISO 22160:2007(E)
IDF 209:2007(E)
©
ISO and IDF 2007
ISO 22160:2007(E)
IDF 209:2007(E)
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Published in Switzerland
ii © ISO and IDF 2007 – All rights reserved

ISO 22160:2007(E)
IDF 209:2007(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Terms and definitions. 1
3 Principle. 1
4 Reagents. 2
5 Apparatus . 3
6 Sampling. 3
7 Preparations . 4
7.1 Test samples . 4
7.2 Alkaline phosphatase-free milk. 4
8 Procedure (see Annex A) . 4
8.1 Calibration . 4
8.2 Calibration adjustment. 6
8.3 Control tests and calibration check. 9
8.4 Determination. 10
8.5 Heat-resistant microbial alkaline phosphatase control. 10
9 Calculation and expression of results. 10
9.1 General. 10
9.2 Manual calculation. 10
9.3 Expression of results . 11
10 Precision. 11
10.1 Interlaboratory test . 11
10.2 Repeatability. 11
10.3 Reproducibility. 12
11 Test report . 12
Annex A (informative) Procedure flowchart . 13
Annex B (informative) Results of interlaboratory trials. 14
Bibliography . 16

ISO 22160:2007(E)
IDF 209:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 22160⎪IDF 209 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5,
Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO and
IDF.
iv © ISO and IDF 2007 – All rights reserved

ISO 22160:2007(E)
IDF 209:2007(E)
Foreword
IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National
Committee in every member country. Every National Committee has the right to be represented on the IDF
Standing Committees carrying out the technical work. IDF collaborates with ISO in the development of
standard methods of analysis and sampling for milk and milk products.
Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the
National Committees for voting. Publication as an International Standard requires approval by at least 50 % of
the IDF National Committees casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. IDF shall not be held responsible for identifying any or all such patent rights.
ISO 22160⎪IDF 209 was prepared by the International Dairy Federation (IDF) and Technical Committee
ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF
and ISO.
All work was carried out by the Joint ISO-IDF Action Team on Heat treatment, of the Standing Committee on
Minor components & characterization of physical properties, under the aegis of its project leader, Mr R. Salter
(US).
ISO 22160:2007(E)
INTERNATIONAL STANDARD
IDF 209:2007(E)
Milk and milk-based drinks — Determination of alkaline
phosphatase activity — Enzymatic photo-activated system
(EPAS) method
1 Scope
This International Standard specifies a method for the determination of the alkaline phosphatase activity in
pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a
chemiluminescent (EPAS) method.
The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats.
The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline
phosphatase activity has less than 7 000 milliunits per litre.
NOTE There has been a successful collaborative trial with cow, sheep, buffalo, and goat whole milk, as well as
skimmed cow milk (< 0,5 % fat), 20 % fat cream and 2 % fat chocol
...