FprEN 17715

Plant biostimulants - Detection of Shigella spp.

This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.

Pflanzen-Biostimulanzien - Nachweis von Shigella spp.

Dieses Dokument liefert ein Verfahren zum Nachweis, dass das Pathogen Shigella spp. in mikrobiellen Pflanzen-Biostimulanzien nicht in einer Konzentration vorhanden ist, die die jeweiligen Grenzwerte überschreitet, die in der EU Düngeprodukteverordnung festgelegt sind.
Das Nachweisverfahren für Shigella-Pathogene ist nicht trennscharf, und eine Quantifizierung wird selten durchgeführt. Der Nachweis erfolgt in der Regel mit einem Anreicherungsmedium und anschließender Subkultivierung auf verschiedenen Selektivmedien.

Biostimulants des végétaux - Recherche de Shigella spp.

Le présent document fournit une méthode permettant de vérifier que le pathogène Shigella spp n’est pas présent dans les biostimulants microbiens des végétaux à une concentration dépassant les limites correspondantes indiquées dans le Règlement UE sur les fertilisants.
La méthode de détection des pathogènes Shigella n’est pas sensible et la quantification est rarement réalisée. La détection est généralement réalisée en utilisant un milieu d’enrichissement, puis en procédant à un repiquage sur une variété de milieux sélectifs.

Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.

General Information

• Status: Not Published
• Publication Date: 02-May-2024
• ICS: 65.080 - Fertilizers
• Technical Committee: CEN/TC 455 - Plant Biostimulants and Agricultural Micro-Organisms
• Drafting Committee: CEN/TC 455/WG 3 - Pathogenic and non-pathogenic microorganisms
• Current Stage: 4599 - Dispatch of FV draft to CMC - Finalization for Vote
• Start Date: 03-Nov-2023
• Due Date: 10-Oct-2023
• Completion Date: 03-Nov-2023
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/564 - Fertilising products in support of Regulation (EU) 2019/1009
• Mandate: M/564 AMD 1 - amending Implementing Decision C(2020) 612 as regards certain harmonised standards and standardisation deliverables, deadlines for their adoption and the duration of the validity of the standardisation request
• Mandate: M/XXX - Commission Implementing Decision on a standardisation request as regards ecodesign requirements for smartphones, mobile phones other than smartphones, cordless phones and slate tablets in support of Regulation (EU) 2023/1670 and Delegated Regulation (EU) 2023/1669
• Ref Project: oSIST prEN 17715:2023 - Plant biostimulants - Detection of Shigella spp.

Relations

• Revises: CEN/TS 17715:2022 - Plant biostimulants - Detection of Shigella spp.
• Effective Date: 08-Feb-2023

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 17715:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.
Plant biostimulants - Detection of Shigella spp.
Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
Biostimulants des végétaux - Détection de Shigella spp.
Ta slovenski standard je istoveten z: prEN 17715
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17715:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 17715:2023

---------------------- Page: 2 ----------------------
oSIST prEN 17715:2023


DRAFT
EUROPEAN STANDARD
prEN 17715
NORME EUROPÉENNE

EUROPÄISCHE NORM

April 2023
ICS 65.080 Will supersede CEN/TS 17715:2022
English Version

Plant biostimulants - Detection of Shigella spp.
Biostimulants des végétaux - Détection de Shigella spp. Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17715:2023 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 17715:2023
prEN 17715:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
4.1 General . 7
4.2 Enrichment in selective liquid medium . 7
4.3 Plating out and identification of colonies. 7
4.4 Biochemical and serological confirmation . 7
5 Culture media, reagents and antisera . 7
6 Apparatus and glassware . 8
7 Sampling . 8
8 Procedure.
...