EN ISO 13366-2:2006

Milk - Enumeration of somatic cells - Part 2: Guidance on the operation of fluoro-opto-electronic counters (ISO 13366-2:2006)

ISO 13366-2|IDF 148-2:2006 gives guidance on the operating conditions for counting somatic cells, in both raw and chemically preserved milk, using fluoro-opto-electronic somatic cell counters in which either a rotating disc technique or flow cytometry is applied in the counting section.
The guidance is applicable to the counting of somatic cells in raw cow milk. The guidance is also applicable to raw milk of other species, such as goat, sheep and buffalo, if the specified prerequisites are met.

Milch - Zählung somatischer Zellen - Teil 2: Leitfaden zum Betrieb fluoreszenzoptoelektronischer Zählgeräte (ISO 13366-2:2006)

Dieser Teil von ISO 13366 | IDF 148 gibt Leitlinien zu den Betriebsbedingungen der Zählung somatischer Zellen in Rohmilch und chemisch konservierter Milch. Die Zählung erfolgt mit einem fluoreszenz¬optoelektronischen Zählgerät für somatische Zellen, in der entweder ein Verfahren mit rotierender Scheibe oder die Durchflusszytometrie im Zählbereich eingesetzt wird.
Der Leitfaden ist anwendbar für die Zählung somatischer Zellen in Rohmilch. Der Leitfaden ist auch für die Zählung in Rohmilch anderer Tierarten wie Ziegen, Schafen und Büffeln anwendbar, falls die festgelegten Vorbedingungen eingehalten werden.

Lait - Dénombrement des cellules somatiques - Partie 2: Lignes directrices pour la mise en oeuvre des compteurs fluoro-opto-électroniques (ISO 13366-2:2006)

L'ISO 13366-2 | FIL 148-2:2006 indique des lignes directrices concernant les conditions opératoires pour le dénombrement des cellules somatiques dans le lait cru et le lait contenant des conservateurs chimiques, à l'aide de compteurs fluoro-opto-électroniques utilisant soit une méthode sur disque rotatif, soit la cytométrie en flux pour la partie comptage.
Les présentes lignes directrices s'appliquent au dénombrement des cellules somatiques dans le lait cru de vache. Elles s'appliquent également au dénombrement dans le lait cru d'animaux d'autres espèces, telles que la chèvre, la brebis et la bufflesse, si les conditions préalables spécifiées sont remplies.

Mleko - Ugotavljanje števila somatskih celic - 2. del: Navodilo za uporabo flouro-opto-elektronskih števcev (ISO 13366-2:2006)

General Information

• Status: Published
• Publication Date: 30-Sep-2006
• Withdrawal Date: 29-Apr-2007
• ICS: 67.100.10 - Milk and processed milk products
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 01-Oct-2006
• Completion Date: 01-Oct-2006
• Ref Project: SIST EN ISO 13366-2:2007 - Milk - Enumeration of somatic cells - Part 2: Guidance on the operation of fluoro-opto-electronic counters (ISO 13366-2:2006)

Relations

• Replaces: EN ISO 13366-3:1997 - Milk - Enumeration of somatic cells - Part 3: Fluoro-opto-electronic method (ISO 13366-3:1997)
• Effective Date: 22-Dec-2008
• Replaces: EN ISO 13366-2:1997 - Milk - Enumeration of somatic cells - Part 2: Electronic particle counter method (ISO 13366-2:1997)
• Effective Date: 22-Dec-2008
• Corrected By: EN ISO 13366-2:2006/AC:2007 - Milk - Enumeration of somatic cells - Part 2: Guidance on the operation of fluoro-opto-electronic counters (ISO 13366-2:2006)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
01-marec-2007
Mleko - Ugotavljanje števila somatskih celic - 2. del: Navodilo za uporabo flouro-
opto-elektronskih števcev (ISO 13366-2:2006)
Milk - Enumeration of somatic cells - Part 2: Guidance on the operation of fluoro-opto-
electronic counters (ISO 13366-2:2006)
Milch - Zählung somatischer Zellen - Teil 2: Leitfaden zum Betrieb
fluoreszenzoptoelektronischer Zählgeräte (ISO 13366-2:2006)
Lait - Dénombrement des cellules somatiques - Partie 2: Lignes directrices pour la mise
en oeuvre des compteurs fluoro-opto-électroniques (ISO 13366-2:2006)
Ta slovenski standard je istoveten z: EN ISO 13366-2:2006
ICS:
67.100.10 0OHNRLQSUHGHODQLPOHþQL Milk and processed milk
SURL]YRGL products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 13366-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2006
ICS 67.100.10 Supersedes EN ISO 13366-2:1997
English Version
Milk - Enumeration of somatic cells - Part 2: Guidance on the
operation of fluoro-opto-electronic counters (ISO 13366-2:2006)
Lait - Dénombrement des cellules somatiques - Partie 2: Milch - Zählung somatischer Zellen - Teil 2: Leitfaden zum
Lignes directrices pour la mise en oeuvre des compteurs Betrieb fluoreszenzoptoelektronischer Zählgeräte (ISO
fluoro-opto-électroniques (ISO 13366-2:2006) 13366-2:2006)
This European Standard was approved by CEN on 29 September 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13366-2:2006: E
worldwide for CEN national Members.

Foreword
This document (EN ISO 13366-2:2006) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and
milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by April 2007, and conflicting national
standards shall be withdrawn at the latest by April 2007.

This document supersedes EN ISO 13366-2:1997.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Endorsement notice
The text of ISO 13366-2:2006 has been approved by CEN as EN ISO 13366-2:2006 without any
modifications.
INTERNATIONAL ISO
STANDARD 13366-2
IDF
148-2
Second edition
2006-10-01
Milk — Enumeration of somatic cells —
Part 2:
Guidance on the operation
of fluoro-opto-electronic counters
Lait — Dénombrement des cellules somatiques —
Partie 2: Lignes directrices pour la mise en œuvre des compteurs
fluoro-opto-électroniques
Reference numbers
ISO 13366-2:2006(E)
IDF 148-2:2006(E)
©
ISO and IDF 2006
ISO 13366-2:2006(E)
IDF 148-2:2006(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. Neither the ISO Central
Secretariat nor the IDF accepts any liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies
and IDF national committees. In the unlikely event that a problem relating to it is found, please inform the ISO Central Secretariat at the
address given below.
©  ISO and IDF 2006
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO or IDF at the respective
address below.
ISO copyright office International Dairy Federation
Case postale 56 • CH-1211 Geneva 20 Diamant Building • Boulevard Auguste Reyers 80 • B-1030 Brussels
Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88
Fax + 41 22 749 09 47 Fax + 32 2 733 04 13
E-mail [e-mail] E-mail [e-mail]
Web www.iso.org Web www.fil-idf.org
Published in Switzerland
ii © ISO and IDF 2006 – All rights reserved

ISO 13366-2:2006(E)
IDF 148-2:2006(E)
Contents Page
Foreword. iv
Foreword. v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Principle. 2
5 Factors affecting the results of measurements. 2
5.1 Sample bottles . 2
5.2 Sampling. 2
5.3 Preservation . 3
5.4 Sample storage and transport. 3
5.5 Interfering substances . 3
5.6 Sample quality at analysis . 4
5.7 Applied chemicals . 4
5.8 Instrument condition . 4
5.9 Working factor. 5
5.10 Testing volumes. 5
6 Calibration . 5
6.1 Reference materials. 5
6.2 Calibration procedure. 6
7 Sampling. 8
8 Determination. 8
9 Checks on performance in routine operation. 8
9.1 Blank checks . 8
9.2 Carry-over effect . 8
9.3 Ratio of reagent volume and test sample volume. 9
9.4 Pilot checks . 9
9.5 Additional instrument surveillance. 9
9.6 Repeatability. 9
9.7 Intralaboratory reproducibility . 10
9.8 External comparisons . 10
10 Specific remarks for the use with milk from different species . 10
10.1 General. 10
10.2 Cow milk . 10
10.3 Goat milk. 10
10.4 Sheep milk . 11
10.5 Buffalo milk . 11
11 Precision. 11
11.1 Repeatability. 11
11.2 Intralaboratory reproducibility . 11
11.3 Reproducibility. 12
12 Test report . 12
Bibliography . 13

ISO 13366-2:2006(E)
IDF 148-2:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13366-2⎪IDF 148-2 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 5, Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO
and IDF.
This edition of ISO 13366-2⎪IDF 148-2 cancels and replaces ISO 13366-2:1997 and ISO 13366-3:1997, which
have been technically revised.
ISO 13366 consists of the following parts, under the general title Milk — Enumeration of somatic cells:
— Part 1: Microsc
...