CEN ISO/TS 10272-3:2010
(Main)Microbiology of food and animal feeding stuffs - Horizontal method for detection and enumeration of Campylobacter spp. - Part 3: Semi-quantitative method (ISO/TS 10272-3:2010)
Microbiology of food and animal feeding stuffs - Horizontal method for detection and enumeration of Campylobacter spp. - Part 3: Semi-quantitative method (ISO/TS 10272-3:2010)
ISO/TS 10272-3:2010 describes a horizontal method for the semi-quantitative determination of Campylobacter spp.
It is applicable to products intended for human consumption or for the feeding of animals, and to environmental samples in the area of food production and food handling. However, it is possible that ISO/TS 10272-3:2010 is not appropriate in every detail for certain products, deviations from it being made necessary for technical reasons. It is possible that ISO/TS 10272-3:2010 is not applicable at all to some other products.
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum Nachweis und zur Zählung von Campylobacter spp. - Teil 3: Semiquantitatives Verfahren (ISO/TS 10272-3:2010)
Dieser Teil von ISO 10272 legt ein horizontales Verfahren zur semiquantitativen Bestimmung von
Campylobacter spp. fest.
Dieses Dokument gilt für Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind, und
für Umgebungsproben im Bereich der Herstellung und der Behandlung von Lebensmitteln. Es ist jedoch
möglich, dass dieser Teil von ISO 10272 nicht bis ins Einzelne für bestimmte Produkte geeignet ist, dass
Abweichungen davon aus fachlichen Gründen erforderlich sind. Es ist möglich, dass dieser Teil von
ISO 10272 auf einige andere Produkte überhaupt nicht anwendbar ist.
Microbiologie des aliments - Méthode horizontale pour la recherche et le dénombrement de Campylobacter spp. - Partie 3: Méthode semi-quantitative (ISO/TS 10272-3:2010)
L'ISO/TS 10272-3:2010 décrit une méthode horizontale pour la détermination semi-quantitative de Campylobacter spp.
Elle est applicable aux produits destinés à une consommation humaine ou aux aliments pour animaux et à des échantillons environnementaux dans le domaine de la production alimentaire et de la manipulation des aliments. Toutefois, il est possible que l'ISO/TS 10272-3:2010 ne convienne pas en tout point pour certains produits, des écarts étant rendus nécessaires par des raisons techniques. Il est possible que l'ISO/TS 10272-3:2010 ne s'applique pas du tout à certains autres produits.
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti in števila bakterij Campylobacter spp. - 3. del: Semikvantitativna metoda (ISO/TS 10272-3:2010)
Ta del ISO 10272 opisuje horizontalno metodo za semikvantitativno metodo določevanja bakterij Campylobacter spp. Velja za proizvode, namenjene za človeško porabo ali za krmljenje živali ter za okoljske vzorce v območjih proizvodnje hrane in ravnanja z hrano. Vendar je možno, da ta del ISO 10272 ni v vseh podrobnostih primeren za določene proizvode, zato so odstopanja zaradi tehničnih razlogov potrebna. Možno je, da ta del ISO 10272 sploh ne velja za nekatere druge proizvode.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2010
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti in
števila bakterij Campylobacter spp. - 3. del: Semikvantitativna metoda (ISO/TS
10272-3:2010)
Microbiology of food and animal feeding stuffs - Horizontal method for detection and
enumeration of Campylobacter spp. - Part 3: Semi-quantitative method (ISO/TS 10272-
3:2010)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum
Nachweis und zur Zählung von Campylobacter spp. - Teil 3: Semiquantitatives Verfahren
(ISO/TS 10272-3:2010)
Microbiologie des aliments - Méthode horizontale pour la recherche et le dénombrement
de Campylobacter spp. - Partie 3: Méthode semi-quantitative (ISO/TS 10272-3:2010)
Ta slovenski standard je istoveten z: CEN ISO/TS 10272-3:2010
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN ISO/TS 10272-3
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
March 2010
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - Horizontal
method for detection and enumeration of Campylobacter spp. -
Part 3: Semi-quantitative method (ISO/TS 10272-3:2010)
Microbiologie des aliments - Méthode horizontale pour la Mikrobiologie von Lebensmitteln und Futtermitteln -
recherche et le dénombrement de Campylobacter spp. - Horizontales Verfahren zum Nachweis und zur Zählung von
Partie 3: Méthode semi-quantitative (ISO/TS 10272-3:2010) Campylobacter spp. - Teil 3: Semiquantitatives Verfahren
(ISO/TS 10272-3:2010)
This Technical Specification (CEN/TS) was approved by CEN on 28 December 2009 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
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© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 10272-3:2010: E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (CEN ISO/TS 10272-3:2010) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the
secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Croatia, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,
Spain, Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO/TS 10272-3:2010 has been approved by CEN as a CEN ISO/TS 10272-3:2010 without any
modification.
TECHNICAL ISO/TS
SPECIFICATION 10272-3
First edition
2010-03-01
Microbiology of food and animal feeding
stuffs — Horizontal method for detection
and enumeration of Campylobacter
spp. —
Part 3:
Semi-quantitative method
Microbiologie des aliments — Méthode horizontale pour la recherche et
le dénombrement de Campylobacter spp. —
Partie 3: Méthode semi-quantitative
Reference number
ISO/TS 10272-3:2010(E)
©
ISO 2010
ISO/TS 10272-3:2010(E)
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ii © ISO 2010 – All rights reserved
ISO/TS 10272-3:2010(E)
Contents Page
Foreword .iv
Introduction.v
1 Scope.1
2 Normative references.1
3 Terms and definitions .1
4 Principle.2
5 Culture media and reagents .2
6 Apparatus.3
7 Sampling.3
8 Preparation of test sample .4
9 Procedure.4
9.1 General .4
9.2 Test portion, initial suspension and dilutions.4
9.3 Enrichment.4
9.4 Isolation.4
9.5 Confirmation of Campylobacter spp. .5
9.6 Identification of Campylobacter spp. (optional).6
10 Calculation and expression of results .8
10.1 Method of calculation.8
10.2 Precision.8
11 Test report.9
Annex A (normative) Diagram of procedure .10
Annex B (normative) Composition and preparation of culture media and reagents.11
Bibliography.17
ISO/TS 10272-3:2010(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
In other circumstances, particularly when there is an urgent market requirement for such documents, a
technical committee may decide to publish other types of document:
⎯ an ISO Publicly Available Specification (ISO/PAS) represents an agreement between technical experts in
an ISO working group and is accepted for publication if it is approved by more than 50 % of the members
of the parent committee casting a vote;
⎯ an ISO Technical Specification (ISO/TS) represents an agreement between the members of a technical
committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting
a vote.
An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a
further three years, revised to become an International Standard, or withdrawn. If the ISO/PAS or ISO/TS is
confirmed, it is reviewed again after a further three years, at which time it must either be transformed into an
International Standard or be withdrawn.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO/TS 10272-3 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
ISO 10272 consists of the following parts, under the general title Microbiology of food and animal feeding
stuffs — Horizontal method for detection and enumeration of Campylobacter spp.:
⎯ Part 1: Detection method
⎯ Part 2: Colony count technique [Technical Specification]
⎯ Part 3: Semi-quantitative method [Technical Specification]
iv © ISO 2010 – All rights reserved
ISO/TS 10272-3:2010(E)
Introduction
Because of the large variety of food and feed products, ISO 10272 may not be appropriate in every detail for
certain products, and for some other products it may be necessary to use different methods.
Nevertheless, in all cases, every attempt should be made to apply ISO 10272 as far as possible, any
deviations being made only if absolutely necessary for technical reasons.
When ISO 10272 is next reviewed, account will be taken of all information then available regarding the extent
to which the methods have been followed and the reasons for deviations from it in the case of particular
products. The harmonization of test methods cannot be immediate and, for certain groups of products,
International Standards and/or national standards may already exist that do not comply with ISO 10272. It is
hoped that, when such standards are reviewed, they will be changed to comply with ISO 10272, so that
eventually the only remaining departures are those necessary for well-established technical reasons.
...
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