EN 14131:2003

Foodstuffs - Determination of folate by microbiological assay

This European Standard specifies a microbiological method for the determination of the total folate content of foodstuffs by turbidimetric detection of the growth of the microorganism Lactobacillus casei, subsp. rhamnosus (ATCC 7469).
The method allows for the determination of folates in foodstuffs, including naturally occurring folates and added folic acid (pteroylglutamic acid).

Lebensmittel - Mikrobiologische Bestimmung von Folat

Diese Europäische Norm legt ein mikrobiologisches Verfahren zur Bestimmung des Gesamtgehaltes an Folat in Lebensmitteln fest, wobei das Wachstums des Mikroorganismus Lactobacillus casei, subsp. rhamnosus (ATCC 7469) durch Trübungsmessung bestimmt wird.
Dieses Verfahren erlaubt die Bestimmung von Folat einschließlich natürlich vorkommender Folate und hinzugefügter Folsäure (Pteroylglutaminsäure) in

Produits alimentaires - Détermination des folates par essai microbiologique

La présente Norme européenne spécifie une méthode microbiologique permettant de déterminer la teneur totale en folates des produits alimentaires par mesure turbidimétrique de la croissance du microorganisme Lactobacillus casei, subsp. rhamnosus (ATCC 7469).
Cette méthode permet la détermination des folates dans les produits alimentaires, y compris les folates naturels et l'acide folique ajouté (acide ptéroylglutamique).

Živila - Določevanje folata z mikrobiološko metodo

General Information

• Status: Published
• Publication Date: 03-Jun-2003
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 9 - Vitamins and Carotenoids
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 17-Sep-2024
• Ref Project: SIST EN 14131:2003 - Foodstuffs - Determination of folate by microbiological assay

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Mikrobiologische Bestimmung von FolatProduits alimentaires - Détermination des folates par essai microbiologiqueFoodstuffs - Determination of folate by microbiological assay07.100.30Mikrobiologija živilFood microbiologyICS:Ta slovenski standard je istoveten z:EN 14131:2003SIST EN 14131:2003en01-november-2003SIST EN 14131:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14131June 2003ICS 07.100.30English versionFoodstuffs - Determination of folate by microbiological assayProduits alimentaires - Détermination des folates par essaimicrobiologiqueLebensmittel - Mikrobiologische Bestimmung von FolatThis European Standard was approved by CEN on 21 April 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14131:2003 ESIST EN 14131:2003

Optional enzyme treatment.16Annex B (informative)
Results of interlaboratory tests.19Bibliography.20SIST EN 14131:2003

1)Bacto Folic Acid Casei Medium is the trade name of a product supplied by Difco. This information is given for theconvenience of users of this European Standard and does not constitute an endorsement by CEN of the product named.Equivalent products may be used if they can be shown to lead to the same results.SIST EN 14131:2003

2)Distributors include National Collection of Industrial and Marine Bacteria Ltd (Aberdeen, UK) and Culture Collection,University of Göteborg (Gothenburg, Sweden). This information is given for the convenience of users of this EuropeanStandard and does not constitute an endorsement by CEN of the distributors named.SIST EN 14131:2003

or water-bath for incubation at (+37 ±0,2) ºC5.4 Spectrometer/nephelometer,
for measurement of turbidity of folate extracts after incubation in testtubes5.5 Test tube racks with cover,
to hold test tubes during heating and incubation5.6 Dialysis tube (optional),
with molecular mass cut-off of 12000 to 14000, for the preparation of ratplasma conjugase (Annex A).5.7 Liquid-handling system (optional),
for automated assay-tube reading5.8 Microplate reader (optional),
for measurement of turbidity after incubation in microplates5.9 Flat-bottomed 96-well microplates, sterile (optional)SIST EN 14131:2003

EN 14131
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