EN ISO 6888-3:2003

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers (ISO 6888-3:2003)

ISO 6888-3:2003 specifies a horizontal method for the enumeration and detection of coagulase-positive staphylococci, using the most probable number (MPN) technique. It is applicable to
products intended for human consumption and the feeding of animals, andenvironmental samples in the area of food production and food handling.
This method is recommended for products where staphylococci are expected to be stressed and in low numbers as, for example, in dried products. Coagulase-positive staphylococci will primarily be Staphylococcus aureus but Staphylococcus intermedius and some strains of Staphylococcus hyicus also produce coagulase.

Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für die Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Species) - Teil 3: Nachweis und MPN-Verfahren für niedrige Keimzahlen (ISO 6888-3:2003)

Dieser Teil der ISO 6888 legt ein horizontales Verfahren für die Zählung und den Nachweis von koagulase-positiven Staphylokokken mit dem MPN-Verfahren (wahrscheinlichste Keimzahl) fest.
Er gilt für
   Produkte, die für den menschlichen Verzehr und als Futtermittel bestimmt sind;
   Umgebungsproben im Bereich der Herstellung von Lebensmitteln und beim Umgang mit Lebensmitteln.
Dieses Verfahren wird für Produkte empfohlen, bei denen Staphylokokken in geschädigter Form und in niedriger Anzahl erwartet werden, wie z. B. bei getrockneten Produkten. Koagulase-positive Staphylokokken sind in erster Linie Staphylococcus aureus. Aber auch Staphylococcus intermedius sowie einige Stämme von Staphylococcus hyicus bilden Koagulase.

Microbiologie des aliments - Méthode horizontale pour le dénombrement des staphylocoques à coagulase positive (Staphylococcus aureus et autres espèces) - Partie 3: Recherche et méthode NPP pour les faibles nombres (ISO 6888-3:2003)

L'ISO 6888-3:2003 spécifie une méthode horizontale pour le dénombrement et la recherche des staphylocoques à coagulase positive par la méthode NPP (nombre le plus probable). Elle est applicable aux
produits destinés à l'alimentation humaine et animale;échantillons d'environnement dans le domaine de la production et de la distribution des aliments.
Cette méthode est recommandée pour les produits susceptibles de contenir des staphylocoques stressés et en faible nombre, comme c'est le cas, par exemple, des produits secs. Les staphylocoques à coagulase positive seront surtout des Staphylococcus aureus, mais les Staphylococcus intermedius et certaines souches de Staphylococcus hyicus peuvent également sécréter de la coagulase libre.

Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila koagolaza pozitivnih stafilokokov (Staphylococcus aureus in druge vrste) - 3. del: Ugotavljanje prisotnosti in MPN-tehnika pri majhnem številu (ISO 6888-3:2003)

General Information

• Status: Published
• Publication Date: 14-Mar-2003
• Withdrawal Date: 29-Sep-2003
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 12-Jun-2009
• Completion Date: 12-Jun-2009
• Ref Project: SIST EN ISO 6888-3:2003 - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers (ISO 6888-3:2003)

Relations

• Corrected By: EN ISO 6888-3:2003/AC:2005 - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers (ISO 6888-3:2003)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2003
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje števila
koagolaza pozitivnih stafilokokov (Staphylococcus aureus in druge vrste) - 3. del:
Ugotavljanje prisotnosti in MPN-tehnika pri majhnem številu (ISO 6888-3:2003)
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of
coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3:
Detection and MPN technique for low numbers (ISO 6888-3:2003)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für die
Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere
Species) - Teil 3: Nachweis und MPN-Verfahren für niedrige Keimzahlen (ISO 6888-
3:2003)
Microbiologie des aliments - Méthode horizontale pour le dénombrement des
staphylocoques a coagulase positive (Staphylococcus aureus et autres especes) - Partie
3: Recherche et méthode NPP pour les faibles nombres (ISO 6888-3:2003)
Ta slovenski standard je istoveten z: EN ISO 6888-3:2003
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 6888-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2003
ICS 07.100.30
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the enumeration of coagulase-positive staphylococci
(Staphylococcus aureus and other species) - Part 3: Detection
and MPN technique for low numbers (ISO 6888-3:2003)
Microbiologie des aliments - Méthode horizontale pour le Mikrobiologie von Lebensmitteln und Futtermitteln -
dénombrement des staphylocoques à coagulase positive Horizontales Verfahren für die Zählung von koagulase-
(Staphylococcus aureus et autres espèces) - Partie 3: positiven Staphylokokken (Staphylococcus aureus und
Recherche et méthode NPP pour les faibles nombres (ISO andere Species) - Teil 3: Nachweis und MPN-Verfahren für
6888-3:2003) niedrige Keimzahlen (ISO 6888-3:2003)
This European Standard was approved by CEN on 28 February 2003.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6888-3:2003 E
worldwide for CEN national Members.

CORRECTED  2003-05-14
Foreword
This document (EN ISO 6888-3:2003) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 275 "Food analysis
- Horizontal methods", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2003, and conflicting national
standards shall be withdrawn at the latest by September 2003.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and the
United Kingdom.
Endorsement notice
The text of ISO 6888-3:2003 has been approved by CEN as EN ISO 6888-3:2003 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of any
of these publications apply to this European Standard only when incorporated in it by amendment or
revision. For undated references the latest edition of the publication referred to applies (including
amendments).
NOTE Where an International Publication has been modified by common modifications, indicated by
(mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 6887-1 1999 Microbiology of food and animal EN ISO 6887-1 1999
feeding stuffs — Preparation of test
samples, initial suspension and
decimal dilutions for microbiological
examination — Part: 1: General rules
for the preparation of the initial
suspension and decimal dilutions
ISO 6888-1 1999 Microbiology of food and animal EN ISO 6888-1 1999
feeding stuffs — Horizontal method
for the enumeration of coagulase-
positive staphylococci
(Staphylococcus aureus and other
species) — Part 1: Technique using
Baird-Parker agar medium
ISO 6888-2 1999 Microbiology of food and animal EN ISO 6888-2 1999
feeding stuffs — Horizontal method
for the enumeration of coagulase-
positive staphylococci
(Staphylococcus aureus and other
species) — Part 2: Technique using
rabbit plasma fibrinogen agar
medium
ISO 8261 2001 Milk and mild products — General EN ISO 8261 2001
guidance for the preparation of test
samples, initial suspensions and
decimal dilutions for microbiological
examination
ISO/TR 11133-1 2000 Microbiology of food and animal ENV ISO 11133-1 2000
feeding stuffs — Guidelines on
preparation and production of culture
media — Part 1: General guidelines
on quality assurance for the
preparation of culture media in the
laboratory
INTERNATIONAL ISO
STANDARD 6888-3
First edition
2003-03-15
Corrected version
2004-01-15
Microbiology of food and animal feeding
stuffs — Horizontal method for the
enumeration of coagulase-positive
staphylococci (Staphylococcus aureus
and other species) —
Part 3:
Detection and MPN technique for low
numbers
Microbiologie des aliments — Méthode horizontale pour le
dénombrement des staphylocoques à coagulase positive
(Staphylococcus aureus et autres espèces) —
Partie 3: Recherche et méthode NPP pour les faibles nombres

Reference number
ISO 6888-3:2003(E)
©
ISO 2003
ISO 6888-3:2003(E)
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ISO 6888-3:2003(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references . 1
3 Terms and definitions. 2
4 Principle . 2
4.1 Detection method. 2
4.2 Enumeration method . 2
5 Diluents and culture media . 3
6 Apparatus. 6
7 Sampling . 6
8 Preparation of test sample. 6
9 Procedure. 6
9.1 Detection method. 6
9.2 Enumeration method . 7
9.3 Selection of plates and interpretation. 8
10 Expression of results. 9
10.1 Detection method. 9
10.2 Enumeration method . 9
11 Precision . 10
12 Test report. 10
Bibliography . 11

ISO 6888-3:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6888-3 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
ISO 6888 consists of the following parts, under the general title Microbiology of food and animal feeding
stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus
and other species):
 Part 1: Technique using Baird-Parker agar medium
 Part 2: Technique using rabbit plasma fibrinogen agar medium
 Part 3: Detection and MPN technique for low numbers
This corrected version of ISO 6888-3:2003 incorporates the following correction:
Subclause 9.1.1
The second paragraph has been amended to resolve any ambiguity.
iv © ISO 2003 — All rights reserved

ISO 6888-3:2003(E
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