EN ISO 10273:2003
(Main)Microbiology of food and animal feedings stuffs - Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica (ISO 10273:2003)
Microbiology of food and animal feedings stuffs - Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica (ISO 10273:2003)
ISO 10273:2003 specifies a horizontal method for the detection of Yersinia enterocolitica presumed to be pathogenic to human subjects. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum Nachweis von präsumtiv pathogenen Yersenia enterocolitica (ISO 10273:2003)
Diese Internationale Norm legt ein horizontales Verfahren zum Nachweis von präsumtiv pathogenen Yersinia enterocolitica fest. Diese Internationale Norm gilt für
Erzeugnisse, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind,
Umgebungsproben im Bereich der Herstellung und der Behandlung von Lebensmitteln
Microbiologie des aliments - Méthode horizontale pour la recherche de Yersinia enterocolitica présumées pathogènes (ISO 10273:2003)
L'ISO 10273:2003 spécifie une méthode horizontale pour la recherche des Yersinia enterocolitica présumées pathogènes chez l'homme. Elle est applicable aux produits pour l'alimentation humaine et animale, ainsi qu'aux échantillons d'environnement pour la production et la distribution des aliments.
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti domnevno patogene Yersinie enterocolitice (ISO 10273:2003)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2003
Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti
domnevno patogene Yersinie enterocolitice (ISO 10273:2003)
Microbiology of food and animal feedings stuffs - Horizontal method for the detection of
presumptive pathogenic Yersinia enterocolitica (ISO 10273:2003)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum
Nachweis von präsumtiv pathogenen Yersenia enterocolitica (ISO 10273:2003)
Microbiologie des aliments - Méthode horizontale pour la recherche de Yersinia
enterocolitica présumées pathogenes (ISO 10273:2003)
Ta slovenski standard je istoveten z: EN ISO 10273:2003
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 10273
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2003
ICS 07.100.30
English version
Microbiology of food and animal feedings stuffs - Horizontal
method for the detection of presumptive pathogenic Yersinia
enterocolitica (ISO 10273:2003)
Microbiologie des aliments - Méthode horizontale pour la Mikrobiologie von Lebensmitteln und Futtermitteln -
recherche de Yersinia enterocolitica présumées Horizontales Verfahren zum Nachweis von präsumtiv
pathogènes (ISO 10273:2003) pathogenen Yersenia enterocolitica (ISO 10273:2003)
This European Standard was approved by CEN on 20 May 2003.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10273:2003 E
worldwide for CEN national Members.
CORRECTED 2003-09-24
Foreword
This document (EN ISO 10273:2003) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 275 "Food
analysis - Horizontal methods", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by December 2003, and conflicting national
standards shall be withdrawn at the latest by December 2003.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and
the United Kingdom.
Endorsement notice
The text of ISO 10273:2003 has been approved by CEN as EN ISO 10273:2003 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of
any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated
by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 6887-1 1999 Microbiology of food and animal EN ISO 6887-1 1999
feeding stuffs - Preparation of test
samples, initial suspension and
decimal dilutions f or
microbiological examination - Part
1: General rules for the
preparation of the initial
suspension and decimal dilutions
ISO 8261 2001 Milk and milk products - General EN ISO 8261 2001
guidance for the preparation of
test samples, initial suspensions
and decimal dilutions for
microbiological examination
ISO/TS 11133-1 2000 Microbiology of food and animal ENV ISO 11133-1 2000
feeding stuffs - Guidelines on
preparation and production of
culture media - Part 1: General
guidelines on quality assurance
for the preparation of culture
media in the laboratory
INTERNATIONAL ISO
STANDARD 10273
Second edition
2003-06-15
Microbiology of food and animal feeding
stuffs — Horizontal method for the
detection of presumptive pathogenic
Yersinia enterocolitica
Microbiologie des aliments — Méthode horizontale pour la recherche de
Yersinia enterocolitica présumées pathogènes
Reference number
ISO 10273:2003(E)
©
ISO 2003
ISO 10273:2003(E)
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ii © ISO 2003 — All rights reserved
ISO 10273:2003(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references . 1
3 Terms and definitions. 1
4 Principle . 2
4.1 General. 2
4.2 Enrichment in selective liquid media. 2
4.3 Plating out and identification. 2
4.4 Confirmation. 2
5 Reagents and media . 2
6 Apparatus and glassware. 4
7 Sampling . 5
8 Preparation of test sample. 5
9 Procedure (see Annex A). 5
9.1 Test portion and initial suspension . 5
9.2 Enrichment. 6
9.3 Plating out and identification. 6
9.4 Confirmation. 6
10 Test report. 12
Annex A (normative) Diagram of procedure. 13
Annex B (normative) Composition and preparation of culture media and reagents . 14
Annex C (informative) Biochemical characteristics at 30 °C of Yersinia pseudotuberculosis,
Yersinia enterocolitica and biochemically related species . 29
Annex D (informative) Biovars (biotyping) of Yersinia enterocolitica . 30
Bibliography . 31
ISO 10273:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 10273 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
This second edition cancels and replaces the first edition (ISO 10273:1994), Subclause 9.4 of which has been
technically revised.
iv © ISO 2003 — All rights reserved
ISO 10273:2003(E)
Introduction
Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods which are specific to these products may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt will be made to apply this
horizontal method as far as possible.
When this International Standard is next reviewed, account will be taken of all information then available
regarding the extent to which this horizontal method has been followed and the reasons for deviations from
this method in the case of particular products.
The harmonization of test methods cannot be immediate, and for certain groups of products International
Standards and/or national standards may already exist that do not comply with this horizontal method. It is
hoped that when such standards are reviewed they will be changed to comply with this International Standard
so that eventually the only remaining departures from this horizontal method will be those necessary for
well-established technical reasons.
INTERNATIONAL STANDARD ISO 10273:2003(E)
Microbiology of food and animal feeding stuffs — Horizontal
me
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