EN ISO 23611-3:2011
(Main)Soil quality - Sampling of soil invertebrates - Part 3: Sampling and soil extraction of enchytraeids (ISO 23611-3:2007)
Soil quality - Sampling of soil invertebrates - Part 3: Sampling and soil extraction of enchytraeids (ISO 23611-3:2007)
ISO 23611-3:2007 specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). It applies to all terrestrial biotopes in which enchytraeids occur.
ISO 23611-3:2007 is not applicable for semi-terrestrial soils and might be difficult to use under extreme climatic or geographical conditions.
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 3: Probenahme und Bodenextraktion von Enchytraeen (ISO 23611-3:2007)
Dieser Teil von ISO 23611 legt ein Verfahren zur Probenahme, Handhabung und Extraktion von Enchytraeen aus terrestrischen Freilandböden fest, mit dessen Hilfe die Voraussetzungen für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) geschaffen werden.
Grundlegende Informationen zur Ökologie von Enchytraeen und ihrer Verwendung als Bioindikatoren in der terrestrischen Umwelt lassen sich den im Literaturverzeichnis aufgeführten Quellen entnehmen.
Dieser Teil von ISO 23611 gilt für alle terrestrischen Biotope, in denen Enchytraeen leben. Der für Freiland-untersuchungen im Allgemeinen gültige Probenahmeplan ist bereits in ISO 10381-1 festgelegt. Diese Einzelheiten können in Abhängigkeit von den klimatischen/regionalen Bedingungen des zu beprobenden Standorts variieren. Referenz [6] vermittelt einen Überblick zur Bestimmung der Wirkungen von Verunreinigungen auf Enchytraeen unter Freilandbedingungen.
Die Verfahren für einige andere Bodenorganismengruppen, wie Regenwürmer und Mikroarthropoden, sind in ISO 23611 1 und ISO 23611 2 festgelegt.
Dieser Teil von ISO 23611 gilt nicht für semi-terrestrische Böden (d. h. in oder nahe reinem Wasser lebend) und kann unter extremen klimatischen oder geographischen Bedingungen (z. B. im Hochgebirge) schwierig anzuwenden sein.
Für die Probenahme von Wirbellosen in Böden wird nachdrücklich empfohlen, den Standort zu charakterisieren (z. B. Berücksichtigung des Klimas und der Bodennutzung). Eine derartige Charakterisierung wird jedoch in diesem Teil von ISO 23611 nicht behandelt. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 sind zur Bestimmung des pH-Wertes, der Korngrößenverteilung, des C/N-Verhältnisses, des organischen Kohlenstoffgehalts und des Wasserrückhaltevermögens geeigneter.
Qualité du sol - Prélèvement des invertébrés du sol - Partie 3: Prélèvement et extraction des enchytréides (ISO 23611-3:2007)
L'ISO 23611-3:2007 spécifie une méthode pour le prélèvement, la manipulation et l'extraction des enchytraéides du sol prélevés sur le terrain comme pré-requis à l'utilisation de ces animaux en tant que bio-indicateurs (par exemple, pour évaluer la qualité d'un sol en tant qu'habitat pour ces organismes). La présente partie de l'ISO 23611 s'applique à tous les biotopes terrestres dans lesquels il est possible de trouver des enchytraéides.
L'ISO 23611-3:2007 n'est pas applicable à des sols semi-terrestres et peut être d'utilisation difficile dans des conditions climatiques ou géographiques extrêmes.
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 3. del: Vzorčenje in ekstrakcija enhitrej iz tal (ISO 23611-3:2007)
ISO 23611-3:2007 določa metodo za vzorčenje, delo in ekstrakcijo enhitrej iz poljskih tal kot nujen pogoj za uporabo teh živali kot bioindikatorjev (npr. za ocenjevanje kakovosti tal kot habitata za organizme). Velja za vse zemeljske biotope, v katerih so enhitreje.
ISO 23611-3:2007 ne velja za polzemeljska tla in je morda težje uporaben v ekstremnih klimatskih ali geografskih razmerah.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 3: Probenahme und Bodenextraktion von Enchytraeen (ISO 23611-3:2007)Qualité du sol - Prélèvement des invertébrés du sol - Partie 3: Prélèvement et extraction des enchytréides (ISO 23611-3:2007)Soil quality - Sampling of soil invertebrates - Part 3: Sampling and soil extraction of enchytraeids (ISO 23611-3:2007)13.080.30Biološke lastnosti talBiological properties of soilsICS:Ta slovenski standard je istoveten z:EN ISO 23611-3:2011SIST EN ISO 23611-3:2012en,fr,de01-januar-2012SIST EN ISO 23611-3:2012SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 23611-3
July 2011 ICS 13.080.30; 13.080.05 English Version
Soil quality - Sampling of soil invertebrates - Part 3: Sampling and soil extraction of enchytraeids (ISO 23611-3:2007)
Qualité du sol - Prélèvement des invertébrés du sol -Partie 3: Prélèvement et extraction des enchytréides (ISO 23611-3:2007)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 3: Probenahme und Bodenextraktion von Enchytraeen (ISO 23611-3:2007) This European Standard was approved by CEN on 17 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 23611-3:2011: ESIST EN ISO 23611-3:2012
Reference numberISO 23611-3:2007(E)© ISO 2007
INTERNATIONAL STANDARD ISO23611-3First edition2007-05-01Soil quality — Sampling of soil invertebrates — Part 3: Sampling and soil extraction of enchytraeids Qualité du sol — Prélèvement des invertébrés du sol — Partie 3: Prélèvement et extraction des enchytréides
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ii © ISO 2007 – All rights reserved
ISO 23611-3:2007(E) © ISO 2007 – All rights reserved iiiContents Page Foreword.iv Introduction.v 1 Scope.1 2 Terms and definitions.1 3 Principle.1 4 Reagents.2 5 Apparatus.2 6 Procedure.3 6.1 Soil sampling.3 6.2 Extraction of the enchytraeids.3 6.3 Microscopic identification.4 6.4 Preservation of Enchytraeidae.4 6.5 Validity of the extraction process.4 6.6 Determination of biomass.5 7 Data assessment.5 8 Test report.5 Annex A (informative)
Species identification in enchytraeids.7 Annex B (informative)
Quick extraction of enchytraeids.8 Bibliography.10
ISO 23611-3:2007(E) iv © ISO 2007 – All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 23611-3 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods. ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates: ⎯ Part 1: Hand-sorting and formalin extraction of earthworms ⎯ Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina) ⎯ Part 3: Sampling and soil extraction of enchytraeids ⎯ Part 4: Sampling, extraction and identification of soil-inhabiting nematodes SIST EN ISO 23611-3:2012
ISO 23611-3:2007(E) © ISO 2007 – All rights reserved vIntroduction This part of ISO 23611 has been drawn up since there is a growing need for the standardization of terrestrial zoological field methods. Such methods, mainly covering the sampling, extraction and handling of soil invertebrates, are needed for the following purposes: ⎯ biological classification of soils including soil quality assessment (e.g. References [21], [25], [27]); ⎯ terrestrial bioindication and long-term monitoring (e.g. References [13], [26]); ⎯ evaluation of the effects of chemicals on soil animals (References [15], [22]). Data for these purposes are gained by standardized methods since they can form the basis for far-reaching decisions (e.g. whether a given site should be remediated or not). In fact, the lack of such standardized methods is one of the most important reasons why biological classification concepts in terrestrial (i.e. soil) habitats have so far been relatively rarely used in comparison to aquatic sites. Originally, the methods described here were developed for taxonomical and ecological studies, investigating the role of enchytraeids in various soil ecosystems. These animals without doubt belong to the most important soil invertebrates in temperate regions (mainly in acidic soils [5]). Their influence on soil functions like litter decomposition and nutrient cycling is well-known [14], [19]. Due to their number which is often very high (and to their population biomass), they are also important in many terrestrial food-webs [4]. Some species have unintentionally been distributed by man in many soils of the world. Since it is neither possible nor useful to standardize methods for all soil organisms, the most important ones have been selected. [Microbiological parameters are already covered by existing ISO guidelines (e.g. ISO 10381-6 [29], ISO 14240-1 [37] and ISO 14240-2 [38])]. SIST EN ISO 23611-3:2012
INTERNATIONAL STANDARD ISO 23611-3:2007(E) © ISO 2007 – All rights reserved 1Soil quality — Sampling of soil invertebrates — Part 3: Sampling and soil extraction of enchytraeids 1 Scope This part of ISO 23611 specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment are included in the Bibliography. This part of ISO 23611 applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies in general is specified in ISO 10381-1. These details can vary according to the climatic/regional conditions of the site to be sampled and an overview on the determination of effects of pollutants on enchytraeids in field situations is given in Reference [6]. Methods for some other soil organism groups such as earthworms or micro-arthropods are specified in ISO 23611-1 and ISO 23611-2. This part of ISO 23611 is not applicable for semi-terrestrial (i.e. living in or close to the pure water) soils and might be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains). When sampling soil invertebrates, it is highly recommendable to characterize the site (e.g. concerning climate and land use). However, such a characterization is not covered by this part of ISO 23611. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 are more suitable for measuring pH, particle size distribution, C/N ratio, organic carbon content and water holding capacity. 2 Terms and definitions For the purposes of this document, the following terms and definitions apply. 2.1 enchytraeids small soil-inhabiting worms (a few millimetres to several centimetres in length) belonging to the family Enchytraeidae, order Oligochaeta (class Clitellata, phylum Annelida) EXAMPLE Species of the genera Enchytraeus, Fridericia or Cogn
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