EN ISO 10519:2026

• Abstract:

  This document specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.

• Status: Not Published
• Publication Date: 13-Oct-2026
• ICS: 67.200.20 - Oilseeds
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6055 - CEN Ratification completed (DOR) - Publishing
• Start Date: 27-Sep-2026
• Completion Date: 27-Sep-2026
• Ref Project: oSIST prEN ISO 10519:2026 - Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/FDIS 10519:2026)

• Consolidated By: ISO 10519:2026 - Rapeseed — Determination of chlorophyll content — Spectrometric method
• Effective Date: 18-Apr-2026
• Revises: EN ISO 10519:2015/A1:2025 - Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor (ISO 10519:2015/Amd 1:2025)
• Effective Date: 08-Apr-2026
• Revises: EN ISO 10519:2015 - Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015)
• Effective Date: 08-Apr-2026

EN ISO 10519:2026 is a CEN and ISO standard for measuring the chlorophyll content of rapeseed by spectrometric analysis. It tells laboratories how to prepare the seed, extract the pigments, measure absorbance, and calculate chlorophyll content for results reported in milligrams per kilogram. The method applies to rapeseed seed and is not for chlorophyll in oils.

What does EN ISO 10519:2026 specify?

EN ISO 10519:2026 specifies a spectrometric method for determining chlorophyll content in rapeseed. In practice, it gives a complete laboratory procedure from sample preparation through to reporting, so users can generate comparable results from seed samples.

The document is organized into clauses on scope, normative references, terms and definitions, principle, reagent, apparatus, sampling, preparation of the test sample, procedure, expression of results, precision, and the test report. It also includes two informative annexes that support method validation and instrument calibration.

Annex	What it covers
Annex A	Results of an interlaboratory test, including precision data
Annex B	Preparation of the calibration curve to determine the k factor

What are the key requirements of EN ISO 10519:2026?

EN ISO 10519:2026 sets out a simple but controlled laboratory method. The important points are the sample condition, the extraction conditions, the measurement wavelengths, and the way results are corrected and reported.

Sample preparation and extraction

Clause 8 requires the laboratory sample to be prepared in accordance with ISO 664 after impurities are removed. Seeds with more than 10 % moisture are dried at 45 °C for 12 h to reduce the risk of chlorophyll loss, then ground to a uniform test sample. This matters because uneven grinding or excess moisture can change the result before testing even starts.

Clause 9 then specifies a 2 g test portion and an extraction with 30 ml of the specified solvent mixture from Clause 5. The method uses a shaking or ball-mill extraction, followed by settling and filtration into a tube for measurement. In practice, this means the operator must keep the extraction conditions consistent if results are to be comparable.

Spectrometric measurement and calculation

Clause 9.3 requires absorbance measurements at 665 nm, 705 nm and 625 nm with a spectrometer suitable for the 600 nm to 700 nm range and a 2 nm spectral bandwidth. The readings at 705 nm and 625 nm are used for baseline correction. This helps reduce the effect of background absorption and improves the usefulness of the 665 nm reading for chlorophyll determination.

Clause 10 gives the calculation of chlorophyll content from corrected absorbance, optical path length, test portion mass and solvent volume. The result is expressed as milligrams per kilogram of product as received, and the moisture content may also be used if the user wants a dry-product basis. The document also notes that the k factor depends on the instrument bandwidth, so some instruments may need calibration rather than use of the default value.

Precision and reporting

Clause 11 summarizes precision from interlaboratory testing and gives repeatability and reproducibility guidance. Clause 12 lists the information that has to appear in the test report, including the sampling method if known, the method used, the result, any checked repeatability, the date, and any operating details or incidents that may have affected the outcome. This is important for quality control, audits, and comparing results between laboratories.

What terms does EN ISO 10519:2026 define?

• Chlorophyll content - The mass fraction of substances in the sample that absorb near 665 nm, expressed as chlorophyll A and reported in milligrams per kilogram.
• Test portion - The weighed portion of the prepared test sample that is taken for extraction and measurement.
• Extraction solvent - The solvent mixture used to remove chlorophyll from the ground rapeseed before spectrometric measurement.
• k factor - The constant used in the calculation of chlorophyll content; Annex B shows how to determine it for a given spectrometer.
• Repeatability - The closeness of results from the same method, same laboratory, same operator and same equipment over a short time.
• Reproducibility - The closeness of results from the same method used in different laboratories with different operators and equipment.

Who uses EN ISO 10519:2026?

EN ISO 10519:2026 is used by testing laboratories, oilseed processors, quality control staff, and buyers who need to measure chlorophyll in rapeseed. It is also relevant to inspection, trade, and plant breeding work where seed quality has to be checked with a standardized laboratory method. The method is most useful when results need to be compared across sites or against a specification.

What changed in EN ISO 10519:2026 from the previous edition?

EN ISO 10519:2026 replaces ISO 10519:2015. The foreword states that this fourth edition is a minor revision and incorporates ISO 10519:2015/Amd 1:2025.

The change explicitly stated is the addition of Annex B, which provides the calibration curve procedure used to determine the k factor.

Which standards are used with EN ISO 10519:2026?

• ISO 648 - Gives the requirements for the single-volume pipettes used in the extraction and calibration work.
• ISO 664 - Covers reduction of the laboratory sample to the test sample.
• ISO 665 - Provides the method for determining moisture and volatile matter when results are needed on a dry-product basis.
• ISO 542 - Is cited as a recommended sampling method for oilseeds.
• ISO 5725:1986 - Is cited in Annex A as the basis for the interlaboratory precision study.

What does the EN ISO 10519:2026 document contain?

The document includes a figure of the mechanical microgrinder in Clause 6, plus the measurement setup for spectrometric analysis. Clause 10 contains the calculation formula for chlorophyll content, including the corrected absorbance terms and the variables used in the result.

Annex A provides the interlaboratory test results behind the precision statements. Annex B gives the calibration procedure and the curve used to determine the k factor for instruments where the default value is not suitable.

SLOVENSKI STANDARD
01-julij-2026
Seme oljne repice - Določevanje klorofila - Spektrometrijska metoda (ISO/FDIS
10519:2026)
Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO/FDIS
10519:2026)
Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren
(ISO/FDIS 10519:2026)
Graines de colza - Détermination de la teneur en chlorophylle - Méthode
spectrométrique (ISO/FDIS 10519:2026)
Ta slovenski standard je istoveten z: prEN ISO 10519
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

FINAL DRAFT
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
2026-06-03
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
2026-08-26
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 10519:2026(en) © ISO 2026

FINAL DRAFT
ISO/FDIS 10519:2026(en)
International
Standard
ISO/FDIS 10519
ISO/TC 34/SC 2
Rapeseed — Determination
Secretariat: AFNOR
of chlorophyll content —
Voting begins on:
Spectrometric method
Graines de colza — Détermination de la teneur en chlorophylle —
Voting terminates on:
Méthode spectrométrique
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
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Phone: [phone]
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Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 10519:2026(en) © ISO 2026

ii
ISO/FDIS 10519:2026(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagent . 1
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 3
9 Procedure . 3
9.1 Test portions .3
9.2 Extraction .3
9.3 Determination .3
10 Expression of results . 4
11 Precision . 4
11.1 Repeatability .4
11.2 Reproducibility .4
12 Test report . 4
Annex A (informative) Results of interlaboratory test . 6
Annex B (informative) Preparation of the calibration curve to determine the k factor . 7
Bibliography . 9

iii
ISO/FDIS 10519:2026(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 2, Oleaginous seeds and fruits and oilseed meals, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
This fourth edition cancels and replaces the third edition (ISO 10519:2015), of which it constitutes a minor
revision. It also incorporates the Amendment ISO 10519:2015/Amd 1:2025.
The changes are as follows:
— Annex B “Preparation of the calibration curve to determine the k factor” has been added.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
FINAL DRAFT International Standard ISO/FDIS 10519:2026(en)
Rapeseed — Determination of chlorophyll content —
Spectrometric method
1 Scope
This document specifies a spectrometric method for the determination of the chlorophyll content of
rapeseed. It is not applicable to the determination of chlorophyll in oils.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 648, Laboratory glassware — Single-volume pipettes
ISO 664, Oilseeds — Reduction of laboratory sample to test sample
ISO 665, Oilseeds — Determination of moisture and volatile matter content
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
chlorophyll content
mass fraction of substances in the sample contributing to the absorption band at a wavelength near 665 nm,
measured as chlorophyll A
Note 1 to entry: The chlorophyll conten
...