Soil quality - Determination of soil microbial biomass - Part 2: Fumigation-extraction method (ISO 14240-2:1997)

Gives a method for the determination of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms. It is also applicable to the estimation of microbial nitrogen and ninhydrin-reactive nitrogen in soil.

Bodenbeschaffenheit - Bestimmung der mikrobiellen Biomasse von Böden - Teil 2: Fumigations-Extraktionsverfahren (ISO 14240-2:1997)

Dieser Teil von ISO 14240 legt ein Verfahren zur Bestimmung der mikrobiellen Biomasse von Böden durch Messung der gesamten extrahierbaren organischen Biomasse aus frisch abgetöteten Mikroorganismen fest. Das Verfahren kann auch für die Bestimmung des mikrobiellen Stickstoffs und mikrobiellen Ninhydrin-reaktiven Stickstoffs in Böden verwendet werden, jedoch ist im Rahmen dieses Teils von ISO 14240 nur die Messung von extrahierbarem organischem Kohlenstoff beschrieben. Das Fumigations-Extraktions-Verfahren ist auf aerobe und anaerobe (vernässte, Reisfeld-) Böden über den gesamten pH-Bereich anwendbar. Die Biomasse kann auch in Böden gemessen werden, die leicht zersetzbare Substrate enthalten und in Böden, die mit Kaliumsulfat-Lösung übersättigt sind.
ANMERKUNG   Die Fumigation mit Chloroform beeinflusst ebenfalls die Bodenfauna. Der Kohlenstoffbeitrag dieser Organismen ist üblicherweise gering (< 5 %) und kann in der Regel vernachlässigt werden.

Qualité du sol - Détermination de la biomasse microbienne du sol - Partie 2 : Méthode par fumigation-extraction (ISO 14240-2:1997)

La présente partie de l'ISO 14240 prescrit une méthode pour l'estimation de la biomasse microbienne des sols par mesurage de la matière extractible totale de biomasse organique, essentiellement à partir de microorganismes venant d'être tués. La méthode est également applicable à l'estimation de l'azote microbien et de l'azote microbien réagissant à la ninhydrine dans les sols; toutefois, seul le mesurage du carbone organique extractible est décrit dans le cadre de la présente partie de l'ISO 14240. La méthode par fumigation-extraction (FE) est applicable aux sols aérobies et anaérobies (saturés, irrigués) dans toute la gamme des pH du sol. La biomasse peut également être mesurée dans des sols contenant des substrats en décomposition active et dans des sols sursaturés de solution de sulfate de potassium.  
NOTE -- La fumigation au chloroforme affecte également la faune du sol. La contribution du carbone de ces organismes est généralement faible (< 5 %) et peut en règle générale être négligée.

Kakovost tal - Določevanje talne mikrobne biomase - 2. del: Ekstrakcijska metoda z zaplinjanjem (ISO 14240-2:1997)

Ta del IS0 14240 določa metodo za ocenjevanje mikrobne biomase v tleh z merjenjem skupne izločljive organske biomase, ki v glavnem prihaja iz sveže ubitih mikroorganizmov. Metoda se uporablja tudi za ocenjevanje mikrobnega dušika in mikrobnega ninhidronsko reaktivnega dušika v tleh, vendar ta del IS0 14240 opisuje le merjenje izločljivega organskega ogljika. Ekstrakcijska metoda z zaplinjanjem (FE) se uporablja za aerobna in anaerobna (prepojena, namočena z vodo) tla v celotnem razponu pH tal. Biomasa se lahko meri tudi v tleh, ki vsebujejo aktivno razpadajoče substrate, in tleh, prenasičenih z raztopino kalijevega sulfata.

General Information

Status
Published
Publication Date
21-Jun-2011
Withdrawal Date
30-Dec-2011
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
22-Jun-2011
Due Date
03-Jun-2012
Completion Date
22-Jun-2011

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Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Bodenbeschaffenheit - Bestimmung der mikrobiellen Biomasse von Böden - Teil 2: Fumigations-Extraktionsverfahren (ISO 14240-2:1997)Qualité du sol - Détermination de la biomasse microbienne du sol - Partie 2 : Méthode par fumigation-extraction (ISO 14240-2:1997)Soil quality - Determination of soil microbial biomass - Part 2: Fumigation-extraction method (ISO 14240-2:1997)13.080.30Biološke lastnosti talBiological properties of soilsICS:Ta slovenski standard je istoveten z:EN ISO 14240-2:2011SIST EN ISO 14240-2:2011en,fr,de01-november-2011SIST EN ISO 14240-2:2011SLOVENSKI
STANDARD



SIST EN ISO 14240-2:2011



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 14240-2
June 2011 ICS 13.080.30 English Version
Soil quality - Determination of soil microbial biomass - Part 2: Fumigation-extraction method (ISO 14240-2:1997)
Qualité du sol - Détermination de la biomasse microbienne du sol - Partie 2 : Méthode par fumigation-extraction (ISO 14240-2:1997)
Bodenbeschaffenheit - Bestimmung der mikrobiellen Biomasse von Böden - Teil 2: Fumigations-Extraktionsverfahren (ISO 14240-2:1997) This European Standard was approved by CEN on 3 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14240-2:2011: ESIST EN ISO 14240-2:2011



EN ISO 14240-2:2011 (E) 2 Contents Page Foreword .3 SIST EN ISO 14240-2:2011



EN ISO 14240-2:2011 (E) 3 Foreword The text of ISO 14240-2:1997 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 14240-2:2011 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2011, and conflicting national standards shall be withdrawn at the latest by December 2011. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 14240-2:1997 has been approved by CEN as a EN ISO 14240-2:2011 without any modification.
SIST EN ISO 14240-2:2011



SIST EN ISO 14240-2:2011



INTERNATIONAL STANDARD IS0 14240-2 First edition 1997-01-I 5 Soil quality - Determination of soil microbial biomass - Part 2: Fumigation-extraction method Qualit6 du sol - Dktermination de la biomasse microbienne du sol - Partie 2: Mkthode d/extraction par fumigation Reference number IS0 14240-2: 1997(E) SIST EN ISO 14240-2:2011



IS0 14240=2:1997(E) 0 IS0 Foreword IS0 (the llnternational Organization for Standardization) is a worldwide federation of national standards bodies (IS0 member bodies). The work of preparing International Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established
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