Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area - Test method and requirements (phase 2, step 1)

This European Standard specifies a test method and the minimum requirements for virucidal activity of chemical disinfectant and antiseptic products that form a homogeneous, physically stable preparation when diluted with hard water or - in the case of ready-to-use-products - with water. Products can only be tested at a concentration of 80 % or less as some dilution is always produced by adding the test organisms and interfering substance.
This European Standard applies to products that are used in the veterinary field i.e. in the breeding, husbandry, production, transport and disposal of all animals except when in the food chain following death and entry to the processing industry.
NOTE 1   The method described is intended to determine the virucidal activity of commercial formulations or active substances under the conditions in which they are used.
NOTE 2   This method corresponds to a phase 2 step 1 test (Annex E).

Chemische Desinfektionsmittel und Antiseptika - Quantitativer Suspensionsversuch zur Bestimmung der viruziden Wirkung chemischer Desinfektionsmittel und Antiseptika für den Veterinärbereich - Prüfverfahren und Anforderungen (Phase 2, Stufe 1)

Die vorliegende Europäische Norm legt ein Prüfverfahren und die Mindestanforderungen an die viruzide Wirkung von chemischen Desinfektionsmitteln und Antiseptika fest, die, bei Verdünnung mit hartem Wasser oder - bei der Verwendung von gebrauchsfertigen Produkten - mit Wasser, eine homogene, physikalisch stabile Zubereitung bilden. Die Produkte können nur bei einer Konzentrationen von 80 % oder darunter geprüft werden, da durch die Zugabe der Prüforganismen und der Belastungssubstanz immer eine Verdünnung hervorgerufen wird.
Diese Europäische Norm gilt für Produkte, die im Veterinärbereich eingesetzt werden, d. h. bei der Aufzucht, Haltung, Produktion, beim Transport und bei der Tierkörperbeseitigung aller Tierarten mit Ausnahme der Bereiche, in denen Tiere direkt nach der Tötung als Nahrungsmittel verwendet oder der weiterverarbeitenden Industrie zugeführt werden.
ANMERKUNG 1   Das beschriebene Verfahren ist zur Bestimmung der viruziden Wirksamkeit von im Handel erhältlichen Zubereitungen oder aktiven Substanzen unter Anwendungsbedingungen vorgesehen.
ANMERKUNG 2   Das Verfahren entspricht einem Versuch der Phase 2/Stufe 1 (siehe Anhang D).
Die vorliegende Norm beschreibt Prüfverfahren für den Rinderenterovirus vom Typ 1 (Enteric Cytopathegenic Orphan [ECBO]-Virus ) als Modellvirus für alle Anwendungen, nämlich zur Desinfektion von Instrumenten und Oberflächen sowie zur Behandlung der Postkontamination von Leichenräumen, Rinnsteinen und Nutzviehställen. Die Auswahl dieses Modellvirus beruht auf:
   der Durchführbarkeit der Aufstellung eines Verfahrens zum Prüfen auf Viren;
   der Repräsentation einer Gruppe klinisch bedeutender Viren;
   der gleichhohen Resistenz gegenüber chemischen und physikalischen Wirkstoffen wie andere klinisch bedeutende Viren im Veterinärbereich.

Antiseptiques et désinfectants chimiques - Essai quantitatif de suspension pour l'évaluation de l'activité virucide des antiseptiques et des désinfectants chimiques utilisés dans le domaine vétérinaire - Méthode d'essai et prescriptions (phase 2, étape 1)

La présente Norme européenne spécifie une méthode d'essai et les prescriptions minimales relatives à l'activité virucide des produits antiseptiques et désinfectants chimiques qui forment une préparation homogène et physiquement stable lorsqu’ils sont dilués avec de l'eau dure ou – dans le cas de produits prêts à l'emploi – avec de l'eau. Les produits ne peuvent être soumis à essai qu'à une concentration inférieure ou égale à 80 % car il se produit toujours une certaine dilution du fait de l’addition des organismes d'essai et de la substance interférente.
La présente Norme européenne s'applique aux produits utilisés dans le domaine vétérinaire c'est-à-dire dans la sélection, l'élevage, la production, le transport et l'élimination de tous les animaux, sauf au cours de la chaîne alimentaire à la suite de leur mort et de leur entrée dans l’industrie de transformation.
NOTE 1   La méthode décrite a pour objet de déterminer l'activité virucide de formulations commerciales ou de substances actives dans leurs conditions d'emploi.
NOTE 2   Cette méthode correspond à un essai de phase 2, étape 1 (voir Annexe E).

Kemična razkužila in antiseptiki - Kvantitativni suspenzijski preskus za vrednotenje virucidnega delovanja kemičnih razkužil in antiseptikov v veterini - Preskusna metoda in zahteve (faza 2, stopnja 1)

General Information

Status
Withdrawn
Publication Date
31-Jan-2006
Withdrawal Date
21-Apr-2015
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
22-Apr-2015

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SLOVENSKI STANDARD
SIST EN 14675:2006
01-junij-2006
.HPLþQDUD]NXåLODLQDQWLVHSWLNL.YDQWLWDWLYQLVXVSHQ]LMVNLSUHVNXV]D
YUHGQRWHQMHYLUXFLGQHJDGHORYDQMDNHPLþQLKUD]NXåLOLQDQWLVHSWLNRYYYHWHULQL
3UHVNXVQDPHWRGDLQ]DKWHYH ID]DVWRSQMD

Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation

of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area -

Test method and requirements (phase 2, step 1)

Chemische Desinfektionsmittel und Antiseptika - Quantitativer Suspensionsversuch zur

Bestimmung der viruziden Wirkung chemischer Desinfektionsmittel und Antiseptika für

den Veterinärbereich - Prüfverfahren und Anforderungen (Phase 2, Stufe 1)
Antiseptiques et désinfectants chimiques - Essai quantitatif de suspension pour

l'évaluation de l'activité virucide des antiseptiques et des désinfectants chimiques utilisés

dans le domaine vétérinaire - Méthode d'essai et prescriptions (phase 2, étape 1)

Ta slovenski standard je istoveten z: EN 14675:2006
ICS:
11.080.20 Dezinfektanti in antiseptiki Disinfectants and antiseptics
11.220 Veterinarstvo Veterinary medicine
SIST EN 14675:2006 en,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 14675:2006
---------------------- Page: 2 ----------------------
SIST EN 14675:2006
EUROPEAN STANDARD
EN 14675
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2006
ICS 11.080.20; 11.220
English Version
Chemical disinfectants and antiseptics - Quantitative suspension
test for the evaluation of virucidal activity of chemical
disinfectants and antiseptics used in the veterinary area - Test
method and requirements (phase 2, step 1)

Antiseptiques et désinfectants chimiques - Essai quantitatif Chemische Desinfektionsmittel und Antiseptika -

de suspension pour l'évaluation de l'activité virucide des Quantitativer Suspensionsversuch zur Bestimmung der

antiseptiques et des désinfectants chimiques utilisés dans viruziden Wirkung chemischer Desinfektionsmittel und

le domaine vétérinaire - Méthode d'essai et prescriptions Antiseptika für den Veterinärbereich - Prüfverfahren und

(phase 2, étape 1) Anforderungen (Phase 2, Stufe 1)
This European Standard was approved by CEN on 26 September 2005.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official

versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,

Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,

Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels

© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 14675:2006: E

worldwide for CEN national Members.
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SIST EN 14675:2006
EN 14675:2006 (E)
Contents Page

Foreword ..................................................................................................................................................................3

Introduction..............................................................................................................................................................4

1 Scope ...........................................................................................................................................................5

2 Normative references .................................................................................................................................5

3 Terms and definitions.................................................................................................................................5

4 Requirements ..............................................................................................................................................7

5 Test methods...............................................................................................................................................7

5.1 Principle.......................................................................................................................................................7

5.2 Materials and reagents ...............................................................................................................................7

5.3 Apparatus and glassware.........................................................................................................................14

5.4 Product test solutions ..............................................................................................................................15

5.5 Procedure for assessing the virucidal activity of the product ..............................................................16

5.6 Infectivity assay ........................................................................................................................................17

5.7 Virucidal test – preparation......................................................................................................................17

6 Calculation and expression of results.....................................................................................................18

6.1 Protocol of the CPE result........................................................................................................................18

6.2 Calculation of infectivity titre (TCID ) ....................................................................................................18

6.3 Calculation of PFU ....................................................................................................................................18

6.4 Verification of the methodology ..............................................................................................................18

6.5 Calculation of the virucidal activity of products.....................................................................................19

6.6 Expression of results................................................................................................................................19

7 Conclusion ................................................................................................................................................19

7.1 General ......................................................................................................................................................19

7.2 Test report .................................................................................................................................................19

Annex A (informative) Referenced strains of national collections ...................................................................21

Annex B (normative) Cytotoxicity, reference inactivation tests and test virus titration .................................22

Annex C (informative) Calculation of the viral infectivity titre and plaque-forming units...............................23

Annex D (informative) Example of a typical test report.....................................................................................25

Annex E (informative) Information on the application and interpretation of European Standards

on chemical disinfectants and antiseptics..............................................................................................26

Bibliography...........................................................................................................................................................28

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SIST EN 14675:2006
EN 14675:2006 (E)
Foreword

This European Standard (EN 14675:2006) has been prepared by Technical Committee CEN/TC 216

“Chemical disinfectants and antiseptics”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by August 2006, and conflicting national standards shall be withdrawn at

the latest by August 2006.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,

Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden,

Switzerland and United Kingdom.
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SIST EN 14675:2006
EN 14675:2006 (E)
Introduction

This European Standard specifies a suspension test for establishing whether a chemical disinfectant or

antiseptic has or does not have a virucidal activity in the fields described in the scope.

The laboratory test closely simulates practical conditions of application. Chosen conditions e.g. contact time,

temperature, test organisms, interfering substances, reflect parameters which are found in practical situations

including conditions which may influence the action of antiseptics or chemical disinfectants.

The conditions are intended to cover general purposes and to allow reference between laboratories and

product types. Each utilization concentration of the chemical disinfectant or antiseptic found by this test

corresponds to defined experimental conditions. However, for some applications the recommendations of use

of a product may differ and therefore additional test conditions need to be used.

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SIST EN 14675:2006
EN 14675:2006 (E)
1 Scope

This European Standard specifies a test method and the minimum requirements for virucidal activity of

chemical disinfectant and antiseptic products that form a homogeneous, physically stable preparation when

diluted with hard water or – in the case of ready-to-use-products – with water. Products can only be tested at a

concentration of 80 % or less as some dilution is always produced by adding the test organisms and

interfering substance.

This European Standard applies to products that are used in the veterinary area i.e. in the breeding,

husbandry, production, transport and disposal of all animals except when in the food chain following death

and entry to the processing industry.

NOTE 1 The method described is intended to determine the virucidal activity of commercial formulations or active

substances under the conditions in which they are used.
NOTE 2 This method corresponds to a phase 2 step 1 test (Annex E).
2 Normative references
Not applicable.
3 Terms and definitions

For the purposes of this European Standard, the following terms and definitions apply.

3.1
product
chemical agent or formulation used as chemical disinfectant or antiseptic
3.2
virucide
product which inactivates viruses under defined conditions
NOTE The adjective derived from ‘virucide' is ‘'virucidal’'.
3.3
virucidal activity

capability of a product to produce a reduction in the number of infectious virus particles of relevant test

organisms under defined conditions
3.4
viral infectivity

ability of a virus to express in cells its genetic information and/or multiply in them to infectious progeny

3.5
inactivation of viruses
reduction of infectivity of a virus by a product

NOTE Alteration of antigenic reactivity or of any viral component does not necessarily mean reduction of infectivity of

a virus.
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SIST EN 14675:2006
EN 14675:2006 (E)
3.6
reference virus suspension

virus suspension of a defined virus strain which is not passaged more than 10 times, is maintained in national

culture collection centres and kept in small volumes (less than 1 ml) at a temperature of −196 °C over liquid

nitrogen
NOTE Stock virus suspensions are prepared from reference virus suspensions.
3.7
stock virus suspension

virus suspension of a defined strain that is multiplied on a large scale to obtain a virus suspension of the same

characteristics as the reference virus suspension and kept in a small volume at a temperature of below −70 °C

or preferably at −196 °C over liquid nitrogen
3.8
test virus suspension
virus suspension that is used in the virucidal testing of the disinfectant
3.9
virus Titre
amount of infectious virus per unit volume present in a cell culture lysate
3.10
reference virus inactivation test

test with a defined reagent (e.g. formalin) instead of a product for the internal control of the test

Results of reference virus inactivation test shall be within limits for validating the method.

3.11
cytotoxicity

morphological alteration of cells and/or their destruction or their reduced sensitivity to virus multiplication

caused by the product
3.12
viral cytopathic effect (CPE)

morphological alteration of cells and/or their destruction as a consequence of virus multiplication

3.13
tissue culture infecting dose (TCID )

virus dose that gives rise to cytopathic change (CPE) in 50 % of the inoculated cell cultures

3.14
viral plaque

area of lysis formed in a cell monolayer under semisolid medium due to infection by and multiplication of a

single infectious virus particle
3.15
plaque forming units (PFU)
number of infectious virus particles per unit volume (ml)
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SIST EN 14675:2006
EN 14675:2006 (E)
4 Requirements

The product when diluted with hard water (5.2.2.3) or – in the case of ready-to-use products – with water

(5.2.2.2) and tested in accordance with Clause 5 under simulated low level soiling (3 g/l bovine albumin

solution) (5.2.2.4.2) or simulated high level soiling (10 g/l bovine albumin solution plus 10 g/l yeast extract)

(5.2.2.4.3) according to its practical applications and under the other obligatory test conditions: 1 selected test

organism, 10 °C, 30 min shall demonstrate at least a lg reduction in virus titre of 4. It is possible to test also

the product as delivered (highest test concentration is 80 %).

The virucidal activity shall be evaluated using the following test organism: Bovine enterovirus Type 1 (ECBO).

Where indicated, additional specific virucidal activity shall be determined applying other contact times,

temperatures and test organisms in accordance with 5.2.1 and 5.5.1.1 in order to take into account intended

specific use conditions.

NOTE For these additional conditions, the concentration defined as a result can be lower than the one obtained

under the obligatory test conditions.
5 Test method
5.1 Principle

5.1.1 A sample of the product as delivered and/or diluted with hard water (or water for ready to use

products) is added to a test suspension of virus in a solution of an interfering substance. The mixture is

maintained at 10 °C ± 1 °C for 30 min ± 10 s (obligatory test conditions).

At the end of the contact time, 0,5 ml of virus/disinfectant mixture is taken. The virucidal activity is immediately

suppressed by dilution in ice-cold diluent. A dilution series with a factor of ten is prepared in an ice-cold

medium held in an ice bath for 10 min. Dilutions shall be prepared in glass tubes (5.3.2.9) or microtitre plates

(5.3.2.8). Pipettes shall be changed after each dilution to avoid carry-over of virus.

The dilutions are transferred into cell culture units (wells of microtitre plates) containing suspended cells. Eight

units shall be inoculated with each dilution. After incubation, the titre of infectivity is calculated. The titration

results of quantal tests shall show dilution steps with the percentage of positive results (presence of CPE or

plaques) lying between 100 % and 0 %. The values are calculated according to Spearman and Kärber (see

Annex C).

Values of virus inactivation are calculated from differences of virus titres before and after treatment with the

product.

5.1.2 Additional and optional contact times and temperatures are specified. Additional test organisms can

be used.
5.2 Materials and reagents
5.2.1 Test virus
The virucidal activity shall be evaluated using the following strain:

 Bovine enterovirus Type 1 (Enteric Cytopathogenic Bovine Orphan Virus – ECBO) ATCC VR-248

1) ATCC VR-248, is a strain supplied by the American Type Culture Collections. This information is given for the

convenience of users of this European Standard and does not constitute an endorsement by CEN of the product named. A

corresponding strain supplied by other culture collections may be used if they can be shown to lead to the same results.

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SIST EN 14675:2006
EN 14675:2006 (E)

NOTE 1 Bovine enterovirus Type 1, strain ECBO, is selected as the model virus for the large Genus Picornavirus. The

Genus Picornavirus includes many clinically important virus species, for example Coxsackie A and B, and enteric

cytopathogenic human orphan (ECHO). Some of these viruses are of primary importance and therefore a constant risk for

animals in the veterinary area. Moreover, they have a high resistance to chemicals, are acid-stable (except rhinoviruses)

and are unaffected by lipid solvents such as ether, and most detergents or quaternary ammonium products.

NOTE 2 It is the model virus for all applications namely for disinfection of instruments and surfaces and post-

contamination treatment of post-mortem rooms, kennels and for animal accommodation.

NOTE 3 Due to large differences of resistance against physical and chemical influences between and within different

virus groups, the testing of all viruses against any particular chemical disinfectant or antiseptic is financially impossible.

Therefore, in this European Standard, testing is restricted to only one so called ‘model virus’ that has been selected on the

basis of the present knowledge as a representative example of virus tenacity and of important clinical relevance in the

veterinary area. If a chemical disinfectant or antiseptic shows virucidal activity according to the requirements of this

European Standard, it may be considered for a phase 2 step 2 test (see Annex E).

NOTE 4 If improvements in the methodology of virus multiplication, virus infectivity or cytoxicity reduction of products

are elaborated, they may be used in parallel with the methodology described in this method to show the improvement.

5.2.2 Culture media and reagents
5.2.2.1 General

All weights of chemical substances given in this European Standard refer to the anhydrous salts. Hydrated

forms may be used as an alternative, but the weights required shall be adjusted to allow for consequent

molecular weight differences.

The reagents shall be of analytical grade and/or appropriate for virological purposes. They shall be free from

substances that are toxic or inhibitory to the test organism.

NOTE 1 To improve reproducibility, it is recommended that commercially available dehydrated material is used for the

preparation of culture media. The manufacturer's instructions relating to the preparation of these products should be

rigorously followed.
NOTE 2 For each culture medium and reagent a limitation for use should be fixed.
5.2.2.2 Water
The water shall be freshly glass distilled water and not demineralised water.
Sterilize in the autoclave [5.3.2.1a)].

NOTE 1 Sterilization is not necessary if the water is used, e.g. for preparation of culture media, and subsequently

sterilized.

NOTE 2 If distilled water of adequate quality is not available, water for injections (see bibliographic reference [2]) can

be used.
NOTE 3 See 5.2.2.3 for the procedure to prepare hard water.
5.2.2.3 Hard water for dilution of products
For the preparation of 1 l of hard water, the procedure is as follows:

 prepare solution A: dissolve 19,84 g magnesium chloride (MgCl ) and 46,24 g calcium chloride (CaCl ) in

2 2

water (5.2.2.2) and dilute to 1 000 ml. Sterilize by membrane filtration [5.3.2.1c)] or in the autoclave

[5.3.2.1a)]. Autoclaving – if used – may cause a loss of liquid. In this case make up to 1 000 ml with water

(5.2.2.2) under aseptic conditions. Store the solution in the refrigerator (5.3.2.16) for no longer than one

month;
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SIST EN 14675:2006
EN 14675:2006 (E)

 prepare solution B: dissolve 35,02 g sodium bicarbonate (NaHCO ) in water (5.2.2.2) and dilute to

1 000 ml. Sterilize by membrane filtration [5.3.2.1c)]. Store the solution in the refrigerator (5.3.2.16) for no

longer than one week;

 place 600 ml to 700 ml of water (5.2.2.2) in a 1 000 ml volumetric flask (5.3.2.13) and add 6,0 ml of

solution A, then 8,0 ml of solution B. Mix and dilute to 1 000 ml with water (5.2.2.2). The pH of the hard

water shall be 7,0 ± 0,2, when measured at 20 °C ± 1°C (5.3.2.4). If necessary, adjust the pH by using a

solution of approximately 40 g/l (about 1 mol/l) of sodium hydroxide (NaOH) or approximately 36,5 g/l

(about 1 mol/l) of hydrochloric acid (HCl).

The hard water shall be freshly prepared under aseptic conditions and used within 12 h.

NOTE When preparing the product test solutions ( ), the addition of the product to the hard water produces a

5.4

different final water hardness in each test tube. In any case the final hardness is lower than 300 mg/l of calcium carbonate

(CaCO ) in the test tube.
5.2.2.4 Interfering substance
5.2.2.4.1 General

The interfering substance shall be chosen according to the conditions of use laid down for the product.

The interfering substance shall be sterile and prepared at 10 times its final concentration in the test.

The ionic composition (e.g. pH, calcium and/or magnesium hardness) and chemical composition (e.g. mineral

substances, protein, carbohydrates, lipids and detergents) shall be defined.

NOTE The term ‘interfering substance’ is used even if it contains more than one substance.

5.2.2.4.2 Low level soiling (Bovine albumin solution)

Dissolve 3 g of bovine albumin (Cohn fraction V for Dubos Medium) in 90 ml of water (5.2.2.2) in a 100 ml

volumetric flask ( ). Make up to the mark with water ( ).
5.3.2.13 5.2.2.2

Sterilize by membrane filtration [5.3.2.1c)]. Keep in a refrigerator (5.3.2.16) and use within one month.

The final concentration of bovine albumin in the test procedure (5.5) is 3 g/l.

5.2.2.4.3 High level soiling (mixture of bovine albumin solution with yeast extract)

Dissolve 50 g yeast extract powder in 150 ml of water (5.2.2.2) in a 250 ml volumetric flask (5.3.2.13) and

allow foam to collapse. Make up to the mark with water (5.2.2.2). Transfer to a clean dry bottle and sterilize in

the autoclave [5.3.2.1a)]. Allow to cool to 20 °C ± 1 °C.

Pipette 25 ml of this solution into a 50 ml volumetric flask (5.3.2.13) and add 10 ml of water (5.2.2.2). Dissolve

5 g of the bovine albumin fraction V in the solution with shaking and allow foam to collapse. Make up to the

mark with water (5.2.2.2) sterilize by membrane filtration [5.3.2.1c)], keep in a refrigerator (at 2 °C to 8 °C)

(5.3.2.16) and use within one month.

The final concentration in the test procedure (5.5) is 10 g/l yeast extract and 10 g/l bovine albumin.

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SIST EN 14675:2006
EN 14675:2006 (E)
5.2.2.5 Antibiotic suspension
Chemicals
50 million units penicillin-G (e.g. Sigma PEN-K )

50 g streptomycin sulphate (approx. equal to 750 i.u./mg) (e.g. Sigma Cat: 56501 )

500,000 units mycostatin (e.g. Nystatin: E R Squibb 59150 )
Water (5.2.2.2) to 2,5 l.
Preparation
Dissolve vial contents in water (5.2.2.2) and make up to 2,5 l.
Dispense aseptically into 50 ml and 5 ml aliquots.
Store at −20 °C. Shake the bottle after thawing.
Use 5 ml per litre of medium to give a final concentration of:
Penicillin 100 units/ml
Streptomycin 100 µg /ml
Mycostatin 25 units/ml
5.2.2.6 Antibiotics-Trypsin-Versene (ATV) 10 ×××× Concentrate
Chemicals
Sodium chloride (NaCl) 80 g
Potassium chloride (KCl) 4 g
Glucose 10 g
Trypsin 5 g (e.g. Difco 1:250 Cat No: 0152-15-9 )
Versene (EDTA) 2 g (e.g. Koch-Light Cat No: 0012424-/B )
0,2 % Phenol Red solution
in water (5.2.2.2) 1 000 ml

2) This information is given for the convenience of users of this European Standard and does not constitute an

endorsement by CEN of the products named. Corresponding products supplied by other manufacturers may be used if

they can be shown to lead to the same results.

3) This information is given for the convenience of users of this European Standard and does not constitute an

endorsement by CEN of the products named. Corresponding products supplied by other manufacturers may be used if

they can be shown to lead to the same results.
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SIST EN 14675:2006
EN 14675:2006 (E)
Preparation

Make up solution to 1 l with water (5.2.2.2) [omitting the sodium bicarbonate and antibiotics (5.2.2.5 and

5.2.2.6).
Filter through a membrane filter (0,22 µm pore size) using positive pressure.

Add 76,2 ml 7,5 % sodium bicarbonate solution in water (5.2.2.2) and 5 ml antibiotics (5.2.2.5) to the sterile

filtrate.
Dispense aseptically into 50 ml aliquots and store at −20 °C.
For use add 50 ml of thawed ATV 10 × concentrate to 450 ml of water (5.2.2.2).
The working strength solution contains trypsin 0,05 %, versene 0,02 %, pH = 7,8.
5.2.2.7 Dulbecco’s Phosphate Buffered Saline pH 7,2 – 7,4 (PBS)
Sodium chloride (NaCl) 8,00 g
Potassium dihydrogen phosphate (KH PO) 0,2 g
2 4
Disodium hydrogen phosphate anhydrous (Na HPO) 0,91 g
2 4
Potassium chloride (KCl) 0,2 g
Water (5.2.2.2) to 1 000 ml
The solution may be sterilised by autoclaving at 121 °C for 15 min.
5.2.2.8 Earle’s balanced salt solution (BSS), 10 ×××× concentrated
Sodium chloride (NaCl) 68,0 g
Magnesium sulphate heptahydrate (MgSO 7HO) 2,0 g
4 2
Sodium dihydrogen orthophosphate monohydrate (NaH PO HO) 1,4 g
2 4 2
Potassium chloride (KCl) 4,0 g
) 2,0 g
Calcium chloride (CaCl
Glucose 10,0 g

The calcium chloride (CaCl ) should be dissolved separately in 100 ml of water (5.2.2.2) and added to the

other dissolved reagents just before the solution is brought to a final volume of 1 000 ml with water (5.2.2.2).

The 10x solution is sterilised by membrane filtration [5.3.2.1c)]. For use, the 10x solution is diluted 1:10 with

sterile water (5.2.2.2) and buffered by the addition of 2,5 % of an 8,8 % sodium bicarbonate solution.

5.2.2.9 Phenol red, 1 %

a) 10 g of alcohol-soluble phenol red is placed in a 100 ml beaker. Approximately 20 ml of 1N sodium

hydroxide (NaOH) solution are added, mixed and allowed to stand for a few minutes;

b) the dissolved dye is transferred into a 1 000 ml volumetric flask (5.3.2.13);
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SIST EN 14675:2006
EN 14675:2006 (E)

c) additional 10 ml of 1N sodium hydroxide (NaOH) solution are added into the beaker. The dissolved

material is transferred into the volumetric flask (5.3.2.13);

d) the solution is brought to a final volume of 1 000 ml with water (5.2.2.2) and stored at room temperature.

5.2.2.10 Eagle’s minimum essential medium (MEM)

The following protocol is given as an example of procedure for the preparation of this cell culture nutrient

medium. It may be also obtained from commercial sources. The concentration of amino acids and vitamins

can be doubled to give “fortified” MEM.

The medium is prepared 10x concentrated and stored in the refrigerator (5.3.2.16). At the time of use

glutamine and antibiotics (stored at −20 °C) and Sodium Bicarbonate are added to the 1x solution.

a) Solution A
per litre 10x medium
L-Arginine HCl 1,05 g
L-Histidine HCl 0,31 g
L-Lysine HCl 0,58 g
L-Tryptophane 0,10 g
L-Phenylalanine 0,32 g
L-Threonine 0,48 g
L-Leucine 0,53 g
L-Valine 0,46 g
L-Isoleucine 0,52 g
L-Methionine 0,15 g
b) Solution B
per litre 10x medium
L-Tyrosine 0,36 g
L-Cysteine 0,24 g

These two amino acids are dissolved in 200 ml of 0,075 N hydrochloric acid with heating at 80 °C.

c) Solution C
Nìcotinamide 0,2 g
Pyridoxal 0,2 g
Thiamine 0,2 g
Pantothenic acid 0,2 g
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SIST EN 14675:2006
EN 14675:2006 (E)
Choline 0,2 g
l-Inositol 0,4 g
Riboflavin 0,02 g

These reagents are dissolved in approximately 175 ml of water (5.2.2.2) then brought to a final volume of

200 ml with water (5.2.2.2). The solution is dispensed in 10 ml volumes and stored at −20 °C.

d) Solution D

Dissolve 200 mg of biotin in 150 ml of water (5.2.2.2). To increase stability upon storage, 1 ml of 1 N

hydrochloric acid is added.

The total volume is brought to 200 ml with water (5.2.2.2) and the solution is dispensed in 10 ml aliquots and

stored at −20 °C.
e) Solution E
Dissolve 200 mg folic acid (crystalline) in 200 ml of lx
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