Plant biostimulants - Determination of the yeast and mould content

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen

Dieses Dokument legt ein horizontales Verfahren zur Zählung von Hefen und Schimmelpilzen in Pflanzen-Biostimulanzien, die zur Verwendung in der Landwirtschaft bestimmt sind, mithilfe des Koloniezähl-verfahrens nach aerober Bebrütung bei (25 ± 2,5) °C fest.
Dieses Dokument hat die Zählung von Hefen und Schimmelpilzen in technischen und formulierten Pflanzen-Biostimulanzien, die im flüssigen oder im festen Zustand vorliegen können, zum Gegenstand. Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, ausgenommen jene, die aus Pilzen oder Hefe bestehen, um zu überprüfen, dass die Konzentration von Hefen und Schimmelpilzen die jeweiligen Grenzwerte, die in der EU Düngeprodukteverordnung [1] angegeben sind, nicht überschreitet.
Gegebenenfalls können die gezählten Hefen und Schimmelpilze durch geeignete Identifizierungsprüfungen identifiziert werden.

Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures

Le présent document spécifie une méthode horizontale pour le dénombrement des levures et des moisissures présentes dans les biostimulants des végétaux destinés à être utilisés en agriculture, par la technique de comptage des colonies après incubation aérobie à 25 °C ± 2,5 °C.
Le présent document permet le dénombrement des levures et des moisissures, dans les biostimulants des végétaux de qualité technique, à la fois à l’état liquide et à l’état solide. La méthode est applicable aux biostimulants microbiens des végétaux, excepté ceux composés de champignons ou de levures, pour vérifier que la concentration des levures et des moisissures ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
Si nécessaire, les levures et les moisissures dénombrées peuvent être identifiées par des essais d’identification appropriés.

Rastlinski biostimulanti - Določanje kvasovk in plesni

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Standards Content (Sample)

oSIST prEN 17707:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti in števila kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Ta slovenski standard je istoveten z: prEN 17707
65.080 Gnojila Fertilizers
oSIST prEN 17707:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17707:2023

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oSIST prEN 17707:2023

prEN 17707


April 2023
ICS 65.080 Will supersede CEN/TS 17707:2022
English Version

Plant biostimulants - Determination of the yeast and
mould content
Biostimulants des végétaux - Détermination de la Pflanzen-Biostimulanzien - Bestimmung des Gehalts an
teneur en levures et en moisissures Hefen und Schimmelpilzen
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17707:2023 E
worldwide for CEN national Members.

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oSIST prEN 17707:2023
prEN 17707:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principles . 8
4.1 General . 8
4.2 Plate count method . 8
5 Diluent and culture media . 8
5.1 General . 8
5.2 Diluent . 8
5.3 Culture media . 9
6 Apparatus and glassware .

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