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CEN

EN 14663:2005

(Main)

Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC

Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC

This document specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC).
Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives as well as the b-glycosylated forms that have been added to foodstuffs, calculated as pyridoxine.
This method has been successfully validated with semolina with milk (infant food), potato puree, vegetables with ham (convenient products) and a multi vitamin drink at levels from 0,034 mg/100 g to 1,21 mg/100 g.

Lebensmittel - Bestimmung von Vitamin B6 (einschließlich glucosidisch gebundener Verbindungen) mit HPLC

Dieses Dokument legt ein Verfahren für die Bestimmung von Vitamin B6 in Lebensmitteln mit Hochleistungs-Flüssigchromatographie
(HPLC) fest.
Vitamin B6 ist der Massenanteil der Summe von Pyridoxin, Pyridoxal und Pyridoxamin einschließlich ihrer
phosphorylierten Verbindungen, wie auch von ß-glycosylierten Verbindungen, berechnet als Pyridoxin.
Dieses Verfahren wurde im Ringversuch erfolgreich für Massenanteile von 0,034 mg/100 g bis 1,21 mg/100 g
in Milch-Griesbrei (Kleinkindernahrung), Kartoffelpüree, Schinken mit Gemüse (Fertignahrung) und einem
Multivitamingetränk validiert.

Produits alimentaires - Dosage de la vitamine B6 (y compris ses formes glycosylées) par CLHP

Le présent document spécifie une méthode de dosage de la vitamine B 6 présente dans les produits
alimentaires, par chromatographie liquide à haute performance (CLHP).
La vitamine B 6 est la fraction massique de la somme de la pyridoxine, du pyridoxal et de la pyridoxamine, y
compris leurs dérivés phosphorylés ainsi que les formes b-glycosylées calculés en tant que pyridoxine.
La présente méthode a été validée avec succès avec de la semoule au lait (aliment pour bébé), de la purée
de pommes de terre, des légumes au jambon (produits tout préparés) et avec une boisson multivitaminée, à
des niveaux de concentration de 0,034 mg/100 g à 1,21 mg/100 g.

Živila – Določevanje vitamina B6 (vključno z glikosilirano obliko) s HPLC

General Information

Status
Published
Publication Date
06-Dec-2005
ICS
67.050 - General methods of tests and analysis for food products
Technical Committee
CEN/TC 275 - Food analysis - Horizontal methods
Drafting Committee
CEN/TC 275/WG 9 - Vitamins and Carotenoids
Current Stage
9093 - Decision to confirm - Review Enquiry
Completion Date
03-Sep-2021
Ref Project
SIST EN 14663:2006 - Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC

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SLOVENSKI STANDARD
SIST EN 14663:2006
01-marec-2006
äLYLOD±'RORþHYDQMHYLWDPLQD% YNOMXþQR]JOLNRVLOLUDQRREOLNR V+3/&
Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC
Lebensmittel - Bestimmung von Vitamin B6 (einschließlich glucosidisch gebundener
Verbindungen) mit HPLC
Produits alimentaires - Dosage de la vitamine B6 (y compris ses formes glycosylées) par
CLHP
Ta slovenski standard je istoveten z: EN 14663:2005
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 14663:2006 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 14663:2006

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SIST EN 14663:2006
EUROPEAN STANDARD
EN 14663
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2005
ICS 67.050

English Version
Foodstuffs - Determination of vitamin B6 (including its
glycosylated forms) by HPLC
Produits alimentaires - Dosage de la vitamine B6 (y Lebensmittel - Bestimmung von Vitamin B6 (einschließlich
compris ses formes glycosylées) par CLHP glucosidisch gebundener Verbindungen) mit HPLC
This European Standard was approved by CEN on 26 October 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 14663:2005: E
worldwide for CEN national Members.

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SIST EN 14663:2006
EN 14663:2005 (E)
Contents Page
Foreword .3
1 Scope.4
2 Normative references.4
3 Principle.4
4 Reagents.4
5 Apparatus.9
6 Procedure.9
7 Calculation.11
8 Precision.12
9 Test report.14
Annex A (informative)  Precision data .15
Annex B (informative) Examples for suitable HPLC-conditions for the determination of vitamin B
6
compounds.19
Annex C (informative)  Examples for molar extinction coefficients .20
Annex D (informative) Figures.21
Bibliography.
...

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