EN ISO 11268-3:2015

Soil quality - Effects of pollutants on earthworms - Part 3: Guidance on the determination of effects in field situations (ISO 11268-3:2014)

ISO 11268-3: 2014 specifies techniques for determining the effects of substances on earthworms in the field and provides a basis for determining the effects of chemicals applied to or incorporated into soil, including soil injections or drilled pelleted formulations.

Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Regenwürmer - Teil 3: Anleitung für die Bestimmung von Wirkungen unter Freilandbedingungen (ISO 11268-3:2014)

Qualité du sol - Effets des polluants vis-à-vis des vers de terre - Partie 3: Lignes directrices relatives à la détermination des effets sur site (ISO 11268-3:2014)

ISO 11268-3:2014 décrit des techniques utilisées pour étudier les effets de substances sur les vers de terre sur le terrain et constitue une base pour la détermination des effets des produits chimiques appliqués ou incorporés au sol, y compris des injections dans le sol ou l'enfouissement de granulés par semoirs.

Kakovost tal - Učinki onesnaževal na deževnike - 3. del: Navodilo za ugotavljanje učinkov v terenskih razmerah (ISO 11268-3:2014)

Ta del standarda ISO 11268 opisuje tehnike za določanje učinkov snovi na deževnike v terenskih razmerah ter zagotavlja podlago za ugotavljanje učinkov kemikalij, uporabljenih na tleh ali vključenih v tla.

General Information

• Status: Published
• Publication Date: 11-Aug-2015
• Withdrawal Date: 28-Feb-2016
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 12-Aug-2015
• Due Date: 08-Oct-2016
• Completion Date: 12-Aug-2015
• Ref Project: SIST EN ISO 11268-3:2015 - Soil quality - Effects of pollutants on earthworms - Part 3: Guidance on the determination of effects in field situations (ISO 11268-3:2014)

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2015
.DNRYRVWWDO8þLQNLRQHVQDåHYDOQDGHåHYQLNHGHO1DYRGLOR]DXJRWDYOMDQMH
XþLQNRYYWHUHQVNLKUD]PHUDK,62
Soil quality - Effects of pollutants on earthworms - Part 3: Guidance on the determination
of effects in field situations (ISO 11268-3:2014)
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Regenwürmer - Teil 3: Anleitung
für die Bestimmung von Wirkungen unter Freilandbedingungen (ISO 11268-3:2014)
Qualité du sol - Effets des polluants vis-à-vis des vers de terre - Partie 3: Lignes
directrices relatives à la détermination des effets sur site (ISO 11268-3:2014)
Ta slovenski standard je istoveten z: EN ISO 11268-3:2015
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 11268-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2015
ICS 13.080.30
English Version
Soil quality - Effects of pollutants on earthworms - Part 3:
Guidance on the determination of effects in field situations (ISO
11268-3:2014)
Qualité du sol - Effets des polluants vis-à-vis des vers de Bodenbeschaffenheit - Wirkungen von Schadstoffen auf
terre - Partie 3: Lignes directrices relatives à la Regenwürmer - Teil 3: Anleitung für die Bestimmung von
détermination des effets sur site (ISO 11268-3:2014) Wirkungen unter Freilandbedingungen (ISO 11268-3:2014)
This European Standard was approved by CEN on 6 August 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11268-3:2015 E
worldwide for CEN national Members.

Contents Page
European foreword .3
European foreword
The text of ISO 11268-3:2014 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 11268-3:2015 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2016, and conflicting national standards shall be withdrawn
at the latest by February 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11268-3:2014 has been approved by CEN as EN ISO 11268-3:2015 without any modification.
INTERNATIONAL ISO
STANDARD 11268-3
Second edition
2014-10-15
Soil quality — Effects of pollutants
on earthworms —
Part 3:
Guidance on the determination of
effects in field situations
Qualité du sol — Effets des polluants vis-à-vis des vers de terre —
Partie 3: Lignes directrices relatives à la détermination des effets sur site
Reference number
ISO 11268-3:2014(E)
©
ISO 2014
ISO 11268-3:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
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Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 11268-3:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Units . 1
4 Principle . 1
5 Reagents and material . 2
6 Apparatus . 2
7 Procedure. 2
7.1 Sampling of earthworm populations . 2
7.2 Preservation . 3
7.3 Determination of biomass . 3
8 Preparation for the test . 3
8.1 Test site . 3
9 Procedure. 6
9.1 Application of test substance . . 6
9.2 Sampling dates . 6
9.3 Reference substance . 7
10 Data assessment. 7
10.1 End points . 7
10.2 Identification of earthworm species . 8
10.3 Determination of biomass with gut content . 8
11 Calculation and expression of results . 8
12 Validity of the test . 8
13 Test report . 9
Annex A (informative) Additional requirements of pesticide testing .10
Annex B (informative) Information on specific earthworm species or communities in
different climatic or geographic regions .11
Bibliography .12
ISO 11268-3:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
This second edition cancels and replaces the first edition (ISO 11268-3:1999), which has been
technically revised.
ISO 11268 consists of the following parts, under the general title Soil quality — Effects of pollutants
on earthworms:
— Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andrei
— Part 2: Determination of effects on reproduction to Eisenia fetida/Eisenia andrei
— Part 3: Guidance on the determination of effects in field situations
iv © ISO 2014 – All rights reserved
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