EN ISO 11268-2:2015

Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)

ISO 11268-2:2012 specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials.
Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil).
Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions.
The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.
This method does not take into account the persistence of the substance during the test.

Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Regenwürmer - Teil 2: Bestimmung der Wirkung auf die Reproduktionsleistung von Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)

Qualité du sol - Effets des polluants vis-à-vis des vers de terre - Partie 2: Détermination des effets sur la reproduction de Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)

L'ISO 11268-2:2012 spécifie l'une des méthodes permettant d'évaluer la fonction d'habitat des sols et de déterminer les effets de contaminants du sol et de produits chimiques sur la reproduction d'Eisenia fetida/Eisenia andrei par absorption cutanée et ingestion. Cet essai chronique s'applique aux sols et aux matériaux de type sol de qualité inconnue, provenant par exemple de sites contaminés, de sols amendés, de sols après remédiation, de sols agricoles ou autres sites d'intérêt et de déchets.
Les effets des substances sont évalués à l'aide d'un sol standard, de préférence un substrat de sol artificiel défini. Pour les sols contaminés, les effets sont déterminés dans le sol soumis à essai et dans un sol témoin. Selon l'objectif de l'étude, le substrat témoin et de dilution (gamme de dilutions d'un sol contaminé) peut être soit un sol non contaminé comparable à l'échantillon de sol à évaluer (sol de référence), soit un sol standard (par exemple un sol artificiel).
Des informations sont fournies sur la manière d'utiliser cette méthode pour évaluer des produits chimiques dans des conditions tempérées ainsi que dans des conditions tropicales.
La méthode ne s'applique pas aux substances volatiles, c'est-à-dire aux substances pour lesquelles H (constante de Henry) ou le coefficient de partage air/eau est supérieur à 1, ou pour lesquelles la pression de vapeur à 25 °C excède 0,013 3 Pa.
Cette méthode ne tient pas compte de la stabilité de la substance pendant l'essai.

Kakovost tal - Učinki onesnaževal na deževnike - 2. del: Določanje učinkov na reprodukcijo Eisenia fetida/Eisenia Andrei (ISO 11268-2:2012)

Ta del standarda EN-ISO 11268 določa eno od metod za ocenjevanje funkcije tal habitata ter določevanje učinkov onesnaževal tal in kemikalij na reprodukcijo Eisenia fetida/Eisenia andrei z dermalnim ali prehranskim vnosom. Ta kronični preskus se uporablja za tla in talne materiale neznane kakovosti, na primer tla iz onesnaženih območij, obogatena tla, tla po sanaciji, kmetijska in druga problematična mesta ter odpadne materiale.
Vpliv snovi se določi z uporabo standardnih tal, najbolje z določenim umetnim talnim substratom. Za kontaminirana tla se učinki določijo v preskušenih in kontrolnih tleh. Glede na cilj raziskave naj bi bila kontrolni in zredčeni substrat (serija redčenja kontaminiranih tal) neokužena tla, primerljiva s tlemi, ki se jih preskuša (referenčna tla), ali standardna tla (npr. umetna tla). Zagotovljene so informacije o uporabi te metode za preskušanje kemikalij v zmernih in tropskih pogojih.
Ta metoda ni primerna za hlapljive snovi, npr. snovi, pri katerih je H (Henryjeva konstanta) ali koeficient deleža zraka/vode večji od 1 ali pri katerih parni tlak pri 25 °C presega 0,0133 Pa. Pri tej metodi se ne upošteva obstojnost snovi med preskusom.

General Information

• Status: Withdrawn
• Publication Date: 11-Aug-2015
• Withdrawal Date: 31-Jan-2023
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Ref Project: SIST EN ISO 11268-2:2015 - Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)

Relations

• Replaced By: EN ISO 11268-2:2023 - Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species (ISO 11268-2:2023)
• Effective Date: 18-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2015
.DNRYRVWWDO8þLQNLRQHVQDåHYDOQDGHåHYQLNHGHO'RORþDQMHXþLQNRYQD
UHSURGXNFLMR(LVHQLDIHWLGD(LVHQLD$QGUHL,62
Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on
reproduction of Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Regenwürmer - Teil 2:
Bestimmung der Wirkung auf die Reproduktionsleistung von Eisenia fetida/Eisenia
andrei (ISO 11268-2:2012)
Qualité du sol - Effets des polluants vis-à-vis des vers de terre - Partie 2: Détermination
des effets sur la reproduction de Eisenia fetida/Eisenia andrei (ISO 11268-2:2012)
Ta slovenski standard je istoveten z: EN ISO 11268-2:2015
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 11268-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2015
ICS 13.080.30
English Version
Soil quality - Effects of pollutants on earthworms - Part 2:
Determination of effects on reproduction of Eisenia fetida/Eisenia
andrei (ISO 11268-2:2012)
Qualité du sol - Effets des polluants vis-à-vis des vers de Bodenbeschaffenheit - Wirkungen von Schadstoffen auf
terre - Partie 2: Détermination des effets sur la reproduction Regenwürmer - Teil 2: Bestimmung der Wirkung auf die
de Eisenia fetida/Eisenia andrei (ISO 11268-2:2012) Reproduktionsleistung von Eisenia fetida/Eisenia andrei
(ISO 11268-2:2012)
This European Standard was approved by CEN on 6 August 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11268-2:2015 E
worldwide for CEN national Members.

Contents Page
European foreword .3

European foreword
The text of ISO 11268-2:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 11268-2:2015 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2016, and conflicting national standards shall be withdrawn
at the latest by February 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11268-2:2012 has been approved by CEN as EN ISO 11268-2:2015 without any modification.
INTERNATIONAL ISO
STANDARD 11268-2
Second edition
2012-11-01
Soil quality — Effects of pollutants on
earthworms —
Part 2:
Determination of effects on reproduction
of Eisenia fetida/Eisenia andrei
Qualité du sol — Effets des polluants vis-à-vis des vers de terre —
Partie 2: Détermination des effets sur la reproduction de Eisenia fetida/
Eisenia andrei
Reference number
ISO 11268-2:2012(E)
©
ISO 2012
ISO 11268-2:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2012 – All rights reserved

ISO 11268-2:2012(E)
Contents Page
Foreword .iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 3
5 Reagents and material . 4
6 Apparatus . 5
7 Procedure . 6
7.1 Experimental design . 6
7.2 Preparation of test mixture . 7
7.3 Addition of the earthworms . 8
7.4 Test conditions and measurements . 8
7.5 Reference substance . 9
8 Calculation and expression of results . 9
8.1 Calculation . 9
8.2 Expression of results . 9
9 Validity of the test . 9
10 Statistical analysis . 9
10.1 General . 9
10.2 Single-concentration tests .10
10.3 Multi-concentration tests .10
11 Test report . 11
Annex A (informative) Determination of the chronic toxicity of chemicals on earthworms under
tropical conditions .13
Annex B (informative) Culturing of Eisenia fetida and Eisenia andrei .15
Annex C (informative) Determination of water holding capacity of artificial soil .16
Annex D (informative) Techniques for counting juvenile worms hatched from cocoons.17
Annex E (informative) Performance of the method .18
Bibliography .20
ISO 11268-2:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11268-2 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
This second edition cancels and replaces the first edition (ISO 11268-2:1998), which has been technically revised.
ISO 11268 consists of the following parts, under the general title Soil quality — Effects of pollutants on earthworms:
— Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andrei
— Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei
— Part 3: Guidance on the determination of effects in field situations
iv © ISO 2012 – All rights reserved

ISO 11268-2:2012(E)
Introduction
Ecotoxicological test systems are applied to obtain information about the effects of contaminants in soil and are
[34] [35]
proposed to complement conventional chemical analysis (see ISO 15799 and ISO 17616 ). ISO 15799
includes a list and short characterization of recommended and standardized test systems and ISO 17616 gives
guidance on the choice and evaluation of the bioassays. Aquatic test systems with soil eluate are applied to
obtain information about the fraction of contaminants potentially reaching the groundwater by the water path
(retention function of soils), whereas terrestrial test systems are used to assess the habitat function of soils. As
standardized test systems using earthworms as indicator organisms for the habitat function of soil, an acute
test for survival and a chronic test for reproduction are available.
This part of ISO 11268 describes a method that is based on the determination of sublethal effects of contaminated
soils on adult earthworms of the species Eisenia fetida (Savigny 1826) and Eisenia andrei (André 1963).
Optionally, the method can be used for testing chemicals added to standard soils (e.g. artificial soil) for their
sublethal hazard potential to earthworms. Finally, information is provided on how to use this method for testing
chemicals under tropical conditions (see Annex A).
Eisenia fetida and Eisenia andrei are considered to be representatives of soil fauna and earthworms in
particular. Background information on the ecology of earthworms and their use in ecotoxicological te
...