Animal feeding stuffs - Determination of Hydrocyanic acid by HPLC

This European Standard is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC).
The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained.

Futtermittel - Bestimmung von Blausäure mittels HPLC

Diese Europäische Norm gilt für die quantitative Bestimmung von (gebundener und freier) Blausäure (HCN) in Futtermittelrohstoffen pflanzlichen Ursprungs und Mischfuttermittel mittels Hochleistungs-Flüssig-chromatographie (HPLC). Das Verfahren ist von 10 mg HCN/kg bis 350 mg HCN/kg validiert. Wird das Verfahren außerhalb dieses Bereiches angewendet, sollte es mindestens innerhalb des Labors validiert werden. Eine Bestimmungsgrenze von 2 mg HCN/kg sollte üblicherweise erreicht werden.

Aliments pour animaux - Dosage de l'acide cyanhydrique par CLHP

La présente Norme européenne est applicable à l’analyse quantitative, par chromatographie liquide à haute performance (CLHP), de l'acide cyanhydrique (HCN) (lié et libre) dans les aliments composés pour animaux et les matières premières d’origine végétale destinées à l’alimentation animale. La méthode est validée pour une concentration en HCN comprise entre 10 mg/kg et 350 mg/kg. Lorsque la méthode est utilisée en dehors de cette gamme, il convient qu’elle soit validée a minima au sein du laboratoire. Il convient normalement d'obtenir une limite de quantification en HCN de 2 mg/kg.

Krma - Določevanje cianovodikove kisline s HPLC

Ta evropski standard se uporablja za kvantitativno analizo (vezane in proste) cianovodikove kisline (HCN) v posamičnih krmilih rastlinskega izvora in krmnih mešanicah s tekočinsko kromatografijo visoke ločljivosti (HPLC). Metoda je validirana za količine od 10 mg HCN/kg do 350 mg HCN/kg. Kadar se metoda uporablja zunaj tega razpona, jo je treba validirati vsaj v laboratoriju. Običajno je treba doseči mejo kvantifikacije 2 mg/kg.

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung von Blausäure mittels HPLCAliments des animaux - Dosage de l'acide cyanhydrique par CLHPAnimal feeding stuffs - Determination of Hydrocyanic acid by HPLC65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16160:2012SIST EN 16160:2012en,fr,de01-september-2012SIST EN 16160:2012SLOVENSKI

SIST EN 16160:2012

EN 16160
March 2012 ICS 65.120 English Version
Animal feeding stuffs - Determination of Hydrocyanic acid by HPLC
Aliments pour animaux - Dosage de l'acide cyanhydrique par CLHP
Futtermittel - Bestimmung von Blausäure mittels HPLC This European Standard was approved by CEN on 14 January 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16160:2012: ESIST EN 16160:2012

EN 16160:2012 (E) 2 Contents Page Foreword .31Scope .42Normative references .43Principle .44Reagents .45Apparatus .76Sampling .87Preparation of test sample .88Procedure .99Calculation of results . 1110Precision . 1211Test report . 12Annex A (informative)
Results of the collaborative study . 13Annex B (informative)
Results of the collaborative study without recovery correction . 14Annex C (informative)
Alternative procedure for enzymatic breakdown (8.3.3)
and steam distillation (8.3.4). 15Bibliography . 16 SIST EN 16160:2012

EN 16160:2012 (E) 3 Foreword This document (EN 16160:2012) has been prepared by Technical Committee CEN/TC 327 “Animal feeding stuffs”, the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publicati

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