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EN 14164:2008

(Main)

Foodstuffs - Determination of vitamin B6 by HPLC

Foodstuffs - Determination of vitamin B6 by HPLC

This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 by which the different vitamers of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard (EN 14166 ) determines the total vitamin B6 by microbiological assay.

Lebensmittel - Bestimmung von Vitamin B6 mit HPLC

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin B6 in Lebensmitteln mit Hochleistungs Flüssigchromatographie (HPLC) fest. Vitamin B6 ist der Massenanteil der Gesamtmenge an Pyridoxin (PN), Pyridoxal (PL) und Pyridoxamin (PM), einschließlich ihrer phosphorylierten Derivate, die als Pyridoxin bestimmt werden. Die ß-glycosylierten Formen werden nicht berücksichtigt. Diese können mit dem in EN 14663 [1] festgelegten Verfahren bestimmt werden, bei dem die verschiedenen Vitamere von Vitamin B6 (Pyridoxin, Pyridoxal und Pyridoxamin) getrennt und individuell bestimmt werden. Eine dritte Europäische Norm (EN 14166) ) [2] bestimmt den Gesamtgehalt an Vitamin B6 mikrobiologisch.

Produits alimentaires - Dosage de la vitamine B6 par CLHP

La présente Norme européenne spécifie une méthode de dosage de la vitamine B6 présente dans les produits
alimentaires par chromatographie liquide à haute performance (CLHP). La vitamine B6 est la fraction
massique de la somme de pyridoxine, pyridoxal, pyridoxamine y compris leurs dérivés phosphorylés identifiés
comme étant de la pyridoxine. Les dérivés ß-glycosilés ne sont pas pris en considération. Ils peuvent être
dosés selon la méthode indiquée dans l’EN 14663 [1], qui permet de séparer et de quantifier les différents
dérivés de la vitamine B6 (pyridoxal, pyridoxamine et pyridoxine). Une troisième Norme européenne
(EN 141661) [2] traite du dosage de la vitamine B6 totale par essai microbiologique.

Živila - Določevanje vitamina B6 s HPLC

General Information

Status
Withdrawn
Publication Date
03-Jun-2008
Withdrawal Date
03-Jun-2014
ICS
67.050 - General methods of tests and analysis for food products
Technical Committee
CEN/TC 275 - Food analysis - Horizontal methods
Drafting Committee
CEN/TC 275/WG 9 - Vitamins and Carotenoids
Current Stage
9960 - Withdrawal effective - Withdrawal
Start Date
04-Jun-2014
Completion Date
04-Jun-2014
Ref Project
SIST EN 14164:2008 - Foodstuffs - Determination of vitamin B6 by HPLC

Relations

Replaces
ENV 14164:2002 - Foodstuffs - Determination of vitamin B6 by HPLC
Effective Date
22-Dec-2008
Replaced By
EN 14164:2014 - Foodstuffs - Determination of vitamin B6 by high performance chromatography
Effective Date
16-Jul-2011

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 14164:2008
01-september-2008
1DGRPHãþD
SIST ENV 14164:2002
äLYLOD'RORþHYDQMHYLWDPLQD%V+3/&
Foodstuffs - Determination of vitamin B6 by HPLC
Lebensmittel - Bestimmung von Vitamin B6 mit HPLC
Produits alimentaires - Dosage de la vitamine B6 par CLHP
Ta slovenski standard je istoveten z: EN 14164:2008
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 14164:2008 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 14164:2008

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SIST EN 14164:2008


EUROPEAN STANDARD
EN 14164

NORME EUROPÉENNE

EUROPÄISCHE NORM
June 2008
ICS 67.050 Supersedes ENV 14164:2002
English Version
Foodstuffs - Determination of vitamin B by HPLC
6
Produits alimentaires - Dosage de la vitamine B par CLHP Lebensmittel - Bestimmung von Vitamin B mit HPLC
6 6
This European Standard was approved by CEN on 5 December 2007.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.






EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 14164:2008: E
worldwide for CEN national Members.

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SIST EN 14164:2008
EN 14164:2008 (E)
Contents Page
Foreword.3
1 Scope .4
2 Normative references .4
3 Principle.4
4 Reagents.4
5 Apparatus .8
6 Procedure .9
7 Calculation.10
8 Test report .10
Annex A (informative) Example of a chromatogram.12
Annex B (informative) Precision data.13
Annex C (informative) Sample treatment option without acid hydrolysis.15
Annex D (informative) Examples for molar absorption coefficients.16
Bibliography .17

2

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SIST EN 14164:2008
EN 14164:2008 (E)
Foreword
This document (EN 14164:2008) has been prepared by Technical Committee CEN/TC 275 “Food analysis -
Horizontal methods”, the secretariat of which is held by DIN.
This European St
...

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