Foodstuffs - Determination of vitamin B6 by HPLC

This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 by which the different vitamers of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard (EN 14166 ) determines the total vitamin B6 by microbiological assay.

Lebensmittel - Bestimmung von Vitamin B6 mit HPLC

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin B6 in Lebensmitteln mit Hochleistungs Flüssigchromatographie (HPLC) fest. Vitamin B6 ist der Massenanteil der Gesamtmenge an Pyridoxin (PN), Pyridoxal (PL) und Pyridoxamin (PM), einschließlich ihrer phosphorylierten Derivate, die als Pyridoxin bestimmt werden. Die ß-glycosylierten Formen werden nicht berücksichtigt. Diese können mit dem in EN 14663 [1] festgelegten Verfahren bestimmt werden, bei dem die verschiedenen Vitamere von Vitamin B6 (Pyridoxin, Pyridoxal und Pyridoxamin) getrennt und individuell bestimmt werden. Eine dritte Europäische Norm (EN 14166) ) [2] bestimmt den Gesamtgehalt an Vitamin B6 mikrobiologisch.

Produits alimentaires - Dosage de la vitamine B6 par CLHP

La présente Norme européenne spécifie une méthode de dosage de la vitamine B6 présente dans les produits
alimentaires par chromatographie liquide à haute performance (CLHP). La vitamine B6 est la fraction
massique de la somme de pyridoxine, pyridoxal, pyridoxamine y compris leurs dérivés phosphorylés identifiés
comme étant de la pyridoxine. Les dérivés ß-glycosilés ne sont pas pris en considération. Ils peuvent être
dosés selon la méthode indiquée dans l’EN 14663 [1], qui permet de séparer et de quantifier les différents
dérivés de la vitamine B6 (pyridoxal, pyridoxamine et pyridoxine). Une troisième Norme européenne
(EN 141661) [2] traite du dosage de la vitamine B6 totale par essai microbiologique.

Živila - Določevanje vitamina B6 s HPLC

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Standards Content (Sample)

SIST EN 14164:2008
SIST ENV 14164:2002
Foodstuffs - Determination of vitamin B6 by HPLC
Lebensmittel - Bestimmung von Vitamin B6 mit HPLC
Produits alimentaires - Dosage de la vitamine B6 par CLHP
Ta slovenski standard je istoveten z: EN 14164:2008
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
SIST EN 14164:2008 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 14164:2008

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SIST EN 14164:2008

EN 14164


June 2008
ICS 67.050 Supersedes ENV 14164:2002
English Version
Foodstuffs - Determination of vitamin B by HPLC
Produits alimentaires - Dosage de la vitamine B par CLHP Lebensmittel - Bestimmung von Vitamin B mit HPLC
6 6
This European Standard was approved by CEN on 5 December 2007.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.



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© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 14164:2008: E
worldwide for CEN national Members.

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SIST EN 14164:2008
EN 14164:2008 (E)
Contents Page
1 Scope .4
2 Normative references .4
3 Principle.4
4 Reagents.4
5 Apparatus .8
6 Procedure .9
7 Calculation.10
8 Test report .10
Annex A (informative) Example of a chromatogram.12
Annex B (informative) Precision data.13
Annex C (informative) Sample treatment option without acid hydrolysis.15
Annex D (informative) Examples for molar absorption coefficients.16
Bibliography .17


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SIST EN 14164:2008
EN 14164:2008 (E)
This document (EN 14164:2008) has been prepared by Technical Committee CEN/TC 275 “Food analysis -
Horizontal methods”, the secretariat of which is held by DIN.
This European St

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