EN ISO 18862:2019
(Main)Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS and GC-MS after derivatization (ISO 18862:2016)
Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS and GC-MS after derivatization (ISO 18862:2016)
ISO 18862:2016 specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction and determination by HPLC-MS/MS and GC-MS. It was validated in a method validation study on roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.
Kaffee und Kaffee-Erzeugnisse - Bestimmung von Acrylamid - Verfahren mittels HPLC-MS/MS und mittels GC-MS nach Derivatisierung (ISO 18862:2016)
Dieses Dokument legt Verfahren zur Bestimmung des Acrylamidgehalts in Kaffee und Kaffee Erzeugnissen durch Wasserextraktion, Aufreinigung durch Festphasen Extraktion und Bestimmung mittels HPLC MS/MS und GC MS fest. Es wurde in einer Verfahrensvalidierungsstudie an Röstkaffee, löslichem Kaffee, Kaffeesurrogaten und Kaffee Erzeugnissen mit Bereichen von 53 μg/kg bis 612,1 μg/kg validiert.
Café et derivés du café - Dosage de l'acrylamide - Méthodes par CLHP-SM/SM et CG-SM après dérivation (ISO 18862:2016)
Le présent document spécifie des méthodes de dosage de l'acrylamide dans le café et les dérivés du café par extraction à l'eau, purification par extraction en phase solide et dosage par CLHP-SM/SM et CG-SM. Il a été validé au cours d'une étude de validation de la méthode réalisée sur du café torréfié, du café soluble, des substituts de café et des dérivés du café dans des plages de concentration allant de 53 μg/kg à 612,1 μg/kg.
Kava in proizvodi iz kave - Določevanje akrilamida - Metode z uporabo HPLC-MS/MS in GC-MS po derivatizaciji (ISO 18862:2016)
ISO 18862:2016 določa metode za določevanje akrilamida v kavi in kavnih izdelkih z ekstrakcijo z vodo, čiščenje s trdnofazno ekstrakcijo ter določevanje s HPLC-MS/MS in GC-MS. Potrjena je bila v študiji potrjevanja metode na praženi kavi, topni kavi, kavnih nadomestkih in kavnih izdelkih z obsegom od 53 μg/kg do 612,1 µg/kg.
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2020
Kava in proizvodi iz kave - Določevanje akrilamida - Metode z uporabo HPLC-
MS/MS in GC-MS po derivatizaciji (ISO 18862:2016)
Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS
and GC-MS after derivatization (ISO 18862:2016)
Kaffee und Kaffee-Erzeugnisse - Bestimmung von Acrylamid - Verfahren mittels HPLC-
MS/MS und mittels GC-MS nach Derivatisierung (ISO 18862:2016)
Café et derivés du café - Détermination de la teneur en acrylamide - Méthodes par
CLHP-SM/SM et CG-SM après dérivation (ISO 18862:2016)
Ta slovenski standard je istoveten z: EN ISO 18862:2019
ICS:
67.140.20 Kava in kavni nadomestki Coffee and coffee substitutes
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 18862
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2019
EUROPÄISCHE NORM
ICS 67.140.20
English Version
Coffee and coffee products - Determination of acrylamide -
Methods using HPLC-MS/MS and GC-MS after
derivatization (ISO 18862:2016)
Café et derivés du café - Dosage de l'acrylamide - Kaffee und Kaffee-Erzeugnisse - Bestimmung von
Méthodes par CLHP-SM/SM et CG-SM après dérivation Acrylamid - Verfahren mittels HPLC-MS/MS und
(ISO 18862:2016) mittels GC-MS nach Derivatisierung (ISO 18862:2016)
This European Standard was approved by CEN on 5 November 2018.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
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COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18862:2019 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 18862:2016 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18862:2019
by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is
held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2020, and conflicting national standards shall be
withdrawn at the latest by April 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18862:2016 has been approved by CEN as EN ISO 18862:2019 without any modification.
INTERNATIONAL ISO
STANDARD 18862
First edition
2016-07-15
Coffee and coffee products —
Determination of acrylamide —
Methods using HPLC-MS/MS and GC-
MS after derivatization
Café et de ses dérivés — Dosage de l’acrylamide — Méthodes utilisant
CLHP-MS/MS et CG-MS après dérivation
Reference number
ISO 18862:2016(E)
©
ISO 2016
ISO 18862:2016(E)
© ISO 2016, Published in Switzerland
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ISO 18862:2016(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 3
7 Sampling . 4
8 Procedure. 4
8.1 General . 4
8.2 Preparation of the sample extract . 4
8.3 Clean-up of the extracts . 5
8.3.1 Carrez precipitation . 5
8.3.2 Solid phase extraction . 5
8.4 HPLC-MS/MS measurement . 5
8.4.1 High-performance liquid chromatography (HPLC) . 5
8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS) . 6
8.5 Measurement with GC-MS . 6
8.5.1 Derivatization and sample preparation for gas chromatography . 6
8.5.2 Gas chromatography . . 7
8.5.3 Identification and quantification by mass spectrometry . 7
9 Calibration . 7
9.1 General advice . 7
9.2 Determination of linearity and definition of the working range . 7
9.3 Calibration with internal standard solution . 7
9.4 Determination of the laboratory specific recovery. 8
10 Evaluation . 8
10.1 Criteria for identification . 8
10.2 Calculation and final results . 8
11 Precision data . 9
11.1 General . 9
11.2 Repeatability . 9
11.3 Reproducibility . 9
11.4 Recovery . 9
12 Measurement uncertainty . 9
13 Test report .10
Annex A (informative) Performance characteristics .11
Annex B (informative) Examples of absorber materials .12
Annex C (informative) Examples of columns and analysis conditions .13
Bibliography .19
ISO 18862:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to de
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