EN ISO 15952:2011
(Main)Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)
Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)
ISO 15952:2005 specifies a semi-static method for the determination of the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added: substances or preparations; and soils (contaminated or of unknown quality) or waste materials. A static method is also described.
ISO 15952:2005 does not apply to volatile substances, i.e. substances for which the Henry constant, H, or the air/water partition coefficient is over 1, or for which the vapour pressure is over 0,013 3 Pa at 25 °C.
This test takes into account possible changes in the test substance, preparation, soil or waste material because the test mixture is prepared and renewed every 7 days during the 28-day test period.
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken - Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO 15952:2006)
Diese Internationale Norm legt ein semistatisches (halbkontinuierliches) Verfahren zur Bestimmung der Wirkungen von Kontaminanten auf das Wachstum und Überleben von jungen Schnecken, üblicherweise Helix aspersa aspersa Müller (Cornu aspersum), fest. Die Tiere werden unter Verwendung eines Prüfsubstrats (künstlicher oder natürlicher Boden in Abhängigkeit von der Zielsetzung der Studie), dem festgelegte Mengen von Folgendem zugesetzt werden, über den Haut und Verdauungspfad exponiert:
- Substanzen oder Zubereitungen;
- Böden (verunreinigte (kontaminierte) oder von unbekannter Beschaffenheit) oder Abfälle.
Zusätzlich zu dem semistatischen Verfahren darf ein statisches Verfahren angewendet werden (optional). Dieses Verfahren ist in Anhang A beschrieben.
Diese Methode ist nicht auf flüchtige Substanzen anwendbar, d. h. Substanzen, bei denen die Henry Konstante H oder der Luft/Wasser Verteilungskoeffizient größer als 1 ist, oder bei denen der Dampf-druck bei 25 °C 0,013 3 Pa überschreitet.
Diese Prüfung berücksichtigt mögliche Veränderungen der Prüfsubstanz, der Zubereitung, des Bodens oder Abfalls, weil im Verlauf der 28 tägigen Prüfdauer die Prüfmischung alle 7 Tage hergestellt und ausgewechselt wird.
Qualité du sol - Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) - Détermination des effets sur la croissance par contamination du sol (ISO 15952:2006)
L'ISO 15952:2005 s'applique à une méthode semi-statique pour la détermination des effets de contaminants sur la croissance et la survie d'escargots juvéniles, généralement Helix aspersa aspersa Müller. Les animaux sont exposés par les voies cutanée et digestive à un substrat d'essai (sol artificiel ou naturel selon l'objectif de l'étude) auquel sont ajoutées des quantités définies de substances ou de préparations, de sols (contaminés ou de qualité inconnue) ou de déchets. Une méthode statique est aussi décrite.
L'ISO 15952:2005 ne s'applique pas aux substances volatiles, c'est-à-dire aux substances dont la constante de Henry, H, ou le coefficient de partage air/eau est supérieur à 1, ou pour lesquelles la pression de vapeur est supérieure à 0,013 3 Pa à 25 °C.
Cet essai prend en considération le changement éventuel de la substance d'essai, de la préparation, du sol ou des déchets, étant donné que le mélange d'essai est préparé et renouvelé tous les 7 jours pendant la période d'essai de 28 jours.
Kakovost tal - Vpliv onesnaževal na juvenilne stadije kopenskih polžev (Helicidae) - Ugotavljanje vplivov na rast zaradi onesnaženja tal (ISO 15952:2006)
ISO 15952:2005 določa semistatično metodo ugotavljanja vpliva onesnaževal na rast in preživetje polžev, po navadi Helix aspersa aspersa Müller, v juvenilnih stadijih. Živali so prek kože in prebavnega trakta izpostavljene preskusnemu substratu (umetnim ali naravnim tlem, glede na namen raziskave), ki se mu dodajo opredeljene količine naslednjega: snovi ali pripravki ter tla (onesnažena ali neznane količine) ali odpadni materiali. Opisana je tudi statična metoda.
ISO 15952:2005 ne velja za hlapne snovi, tj. snovi, pri katerih je Henryjeva konstanta, H, ali porazdelitveni koeficient zrak/voda večji od 1 ali pri katerih parni tlak presega 0,013 3 Pa pri 25 °C.
Ta preskus upošteva morebitne spremembe v preskusni snovi, pripravku, tleh ali odpadnem materialu, ker se preskusna zmes pripravlja in obnavlja vsakih sedem dni med 28-dnevnim preskusnim obdobjem.
General Information
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Kakovost tal - Vpliv onesnaževal na juvenilne stadije kopenskih polžev (Helicidae) - Ugotavljanje vplivov na rast zaradi onesnaženja tal
(ISO 15952:2006)Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken - Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO 15952:2006)Qualité du sol - Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) - Détermination des effets sur la croissance par contamination du sol (ISO 15952:2006)Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)13.080.30Biološke lastnosti talBiological properties of soilsICS:Ta slovenski standard je istoveten z:EN ISO 15952:2011SIST EN ISO 15952:2012en,fr,de01-januar-2012SIST EN ISO 15952:2012SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 15952
June 2011 ICS 13.080.30 English Version
Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)
Qualité du sol - Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) - Détermination des effets sur la croissance par contamination du sol (ISO 15952:2006)
Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken - Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO 15952:2006) This European Standard was approved by CEN on 10 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 15952:2011: ESIST EN ISO 15952:2012
Reference numberISO 15952:2006(E)© ISO 2006
INTERNATIONAL STANDARD ISO15952First edition2006-02-15Soil quality — Effects of pollutants on juvenile land snails (Helicidae) — Determination of the effects on growth by soil contamination Qualité du sol — Effets des polluants vis-à-vis des escargots juvéniles (Helicidae) — Détermination des effets sur la croissance par contamination du sol
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ii © ISO 2006 – All rights reserved
ISO 15952:2006(E) © ISO 2006 – All rights reserved iiiContents Page Foreword.iv Introduction.v 1 Scope.1 2 Normative references.1 3 Terms and definitions.2 4 Principle.3 5 Test environment.3 6 Reagents.3 7 Apparatus.5 8 Storage and preparation of the samples.6 8.1 Soil to be tested.6 8.2 Waste material.6 9 Procedure.6 9.1 Preparation of the test.6 9.2 Distribution of the test mixture.8 9.3 Introduction of the feed.8 9.4 Introduction of the biological reagent.8 9.5 Handling during the tests.8 10 Reference substance.10 11 Calculations and expression of results.10 11.1 Calculations.10 11.2 Expression of results.12 12 Validity of test for Helix aspersa aspersa.13 13 Test report.13 Annex A (normative)
Static method.15 Annex B (informative)
Breeding technique for snails.16 Annex C (informative)
Example of composition of snail feed.21 Annex D (informative)
Example of table of data.22 Annex E (informative)
Example of results with Helix aspersa aspersa.23 Annex F (informative)
Determination of the effects on growth by food contamination.26 Annex G (informative)
Test performance with other snail species.30 Bibliography.31
ISO 15952:2006(E) iv © ISO 2006 – All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 15952 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods. SIST EN ISO 15952:2012
ISO 15952:2006(E) © ISO 2006 – All rights reserved vIntroduction Because of the limited amount of data available concerning toxicity of contaminants on soil organisms, the problems of assessing the ecotoxicity of soils and waste are cause for serious concern at both national and international levels. Currently available tests use soil-fauna organisms restricted to annelid (earthworms and Enchytraeidae) and arthropod phyla (insects: Collembola and Coleoptera). Among the latter, two standards assess acute toxicity [earthworms (ISO 11268-1) and coleoptera larvae [5]] and three other standards assess sublethal effects of soil contaminants on reproduction (earthworms [2], Collembola [1], Enchytraeidae [3]). In the biological cycles of organisms, it appears that growth is, like reproduction, a fundamental ecophysiological parameter to be taken into consideration for the sustainability of species and ecosystems [33]. Snails are pertinent ecological indicators for assessing the quality of soils [15], as they are characteristic of the soil surface layer (saprophagous and phytophagous) of which a large part of the biological cycle takes place in the soil (egg-laying, hatching, initial stages of development, hibernation, etc.) [6], [17], [26]. During the other phases of their cycle, they eat soil and are in contact with the soil via their moist pedal sole (foot) covered with mucus and participate in the permanent exchanges with the soil (water, mineral salts, excrement and finally shell and organic matter when they die) [6], [17], [28]. In addition, they constitute an important link between plants, fauna and soil microorganisms. They correspond fully to the criteria for a good biological indicator: easy to sample and identify, they are widely distributed; they accumulate contaminants [8, 10 to 14, 16, 17, 19, 21, 26, 27, 35 to 43]; their ecological and physiological characteristics are well-known [6], [9], [29]; and they are now easy to breed under controlled conditions [19], [23, [29]. Their susceptibility to common contaminants of their environment has been demonstrated [10 to 15, 18 to 27, 32, 33, 36 to 42]. This International Standard describes a method for determining the effects on survival
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