Plant biostimulants - Detection of Vibrio spp.

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
-   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory must validate the procedure and data generated.

Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.

Dieses Dokument legt ein horizontales Verfahren zum Nachweis von enteropathogenen Vibrio spp. fest, die Erkrankungen des Menschen am oder über den Darmtrakt verursachen [1]. Die Spezies, deren Nachweis die hier aufgeführten Verfahren einbeziehen, sind Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus.
Das Dokument gilt für
-   mikrobielle Pflanzen-Biostimulanzien.
ANMERKUNG 1   Die Weltgesundheitsorganisation (WHO; en: World Health Organization) hat V. parahaemolyticus, V. cholerae und V. vulnificus als die wichtigsten Kontaminanten von Vibrio spp. identifiziert [1].
ANMERKUNG 2   Eine Bestätigung durch PCR Tests ist möglich; in diesem Fall muss das Labor das Verfahren und die erzeugten Daten validieren.

Biostimulants des végétaux - Détection de Vibrio spp.

Le présent document spécifie une méthode horizontale pour la recherche des espèces entéropathogènes de Vibrio provoquant des maladies dans ou via le tractus intestinal chez l’homme [1]. Les espèces détectables par les méthodes spécifiées incluent Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus.
Il s’applique :
-   aux biostimulants microbiens des végétaux.
NOTE 1   L’Organisation Mondiale de la Santé (OMS) a identifié V. parahaemolyticus, V. cholerae et V. vulnificus comme les principales espèces de Vibrio contaminantes [1].
NOTE 2   Pour la confirmation, il est possible de recourir aux essais de PCR ; dans ce cas, le laboratoire doit valider le mode opératoire et les données générées.

Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.

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Standards Content (Sample)

oSIST prEN 17711:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
Plant biostimulants - Detection of Vibrio spp.
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp.
Ta slovenski standard je istoveten z: prEN 17711
65.080 Gnojila Fertilizers
oSIST prEN 17711:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17711:2023

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oSIST prEN 17711:2023

prEN 17711


April 2023
ICS 65.080 Will supersede CEN/TS 17711:2022
English Version

Plant biostimulants - Detection of Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp. Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17711:2023 E
worldwide for CEN national Members.

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oSIST prEN 17711:2023
prEN 17711:2023 (E)
Contents Page
European foreword . 6
Introduction . 7
1 Scope . 8
2 Normative references . 8
3 Terms and definitions . 8
4 Principle . 9
4.1 General . 9
4.2 Primary enrichment in a liquid selective medium . 9
4.3 Secondary enrichment in a liquid selective medium . 9
4.4 Isolation and identification . 10
4.5 Confirmation . 10
5 Culture media and reagents . 10
5.1 Enrichment medium: alkaline saline peptone water (ASPW) . 10
5.2 Solid selective isolation media . 10
5.2.1 First medium: thiosulphate, citrate, bile and sucrose agar medium (TCBS) .

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