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CEN

prEN ISO 13136-1

(Main)

Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024)

Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024)

This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening.
This document is applicable to
—   products intended for human consumption,
—   products intended for animal feeding,
—   environmental samples in the area of food and feed production, handling, and
—   samples from the primary production stage.

Mikrobiologie der Lebensmittelkette - Nachweis, Isolierung und Charakterisierung von Shiga-Toxin bildenden Escherichia coli (STEC) - Teil 1: Horizontales Verfahren zum Nachweis und zur Isolierung von Shiga-Toxin bildenden Escherichia coli (STEC) (ISO/DIS 13136‑1:2024)

Microbiologie de la chaîne alimentaire - Détection, isolement et caractérisation des Escherichia coli producteurs de Shigatoxines (STEC) - Partie 1: Méthode horizontale pour la détection et l’isolement des Escherichia coli producteurs de Shigatoxines (STEC (ISO/DIS 13136-1:2024)

Mikrobiologija v prehranski verigi - Odkrivanje prisotnosti, izolacija in karakterizacija Escherichia coli (STEC), ki proizvaja Shiga toksin - 1. del: Horizontalna metoda za odkrivanje prisotnosti in izolacijo Escherichia coli (STEC), ki proizvaja Shiga toksin (ISO/DIS 13136-1:2024)

General Information

Status
Not Published
Publication Date
10-Jun-2025
ICS
07.100.30 - Food microbiology
Technical Committee
CEN/TC 463 - Microbiology of the food chain
Drafting Committee
CEN/TC 463/WG 2 - Shiga toxin producing Escherichia coli (STEC)
Current Stage
4020 - Submission to enquiry - Enquiry
Start Date
15-Feb-2024
Due Date
10-Jun-2023
Completion Date
15-Feb-2024
Ref Project
oSIST prEN ISO 13136-1:2024 - Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024)

Relations

Revises
CEN ISO/TS 13136:2012 - Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)
Effective Date
17-Aug-2022

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Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN ISO 13136-1:2024
01-april-2024
Mikrobiologija v prehranski verigi - Odkrivanje prisotnosti, izolacija in
karakterizacija Escherichia coli (STEC), ki proizvaja Shiga toksin - 1. del:
Horizontalna metoda za odkrivanje prisotnosti in izolacijo Escherichia coli (STEC),
ki proizvaja Shiga toksin (ISO/DIS 13136-1:2024)
Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-
producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and
isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024)
Mikrobiologie der Lebensmittelkette - Nachweis, Isolierung und Charakterisierung von
Shiga-Toxin bildenden Escherichia coli (STEC) - Teil 1: Horizontales Verfahren zum
Nachweis und zur Isolierung von Shiga-Toxin bildenden Escherichia coli (STEC)
(ISO/DIS 13136-1:2024)
Microbiologie de la chaîne alimentaire - Détection, isolement et caractérisation des
Escherichia coli producteurs de Shigatoxines (STEC) - Partie 1: Méthode horizontale
pour la détection et l’isolement des Escherichia coli producteurs de Shigatoxines (STEC
(ISO/DIS 13136-1:2024)
Ta slovenski standard je istoveten z: prEN ISO 13136-1
ICS:
07.100.30 Mikrobiologija živil Food microbiology
oSIST prEN ISO 13136-1:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 13136-1:2024

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oSIST prEN ISO 13136-1:2024
DRAFT
International
Standard
ISO/DIS 13136-1
ISO/TC 34/SC 9
Microbiology of the food
Secretariat: AFNOR
chain — Detection, isolation and
Voting begins on:
characterization of Shiga toxin-
2024-02-14
producing Escherichia coli (STEC) —
Voting terminates on:
2024-05-08
Part 1:
Horizontal method for the detection
and isolation of Shiga toxin-
producing Escherichia coli (STEC)
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference number
ISO/DIS 13136-1:2024(en)
© ISO 2024

---------------------- Page: 3 ----------------------
oSIST prEN ISO 13136-1:2024
DRAFT
ISO/DIS 13136-1:2024(en)
International
Standard
ISO/DIS 13136-1
ISO/TC 34/SC 9
Microbiology of the food
Secretariat: AFNOR
chain — Detection, isolation and
Voting begins on:
characterization of Shiga toxin-
producing Escherichia coli (STEC) —
Voting terminates on:
Part 1:
Horizontal method for the detection
and isolation of Shiga toxin-
producing Escherichia coli (STEC)
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
COPYRIGHT PROTECTED DOCUMENT
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: +41 22 749 01 11
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/DIS 13136-1:2023(en)
© ISO 2023
© ISO 2024 – All rights reserved
ii

---------------------- Page: 4 ----------------------
oSIST prEN ISO 13136-1:2024
ISO/DIS 13136-1:2023(en)
Contents Page
Foreword .
...

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