EN ISO 13162:2015
(Main)Water quality - Determination of carbon 14 activity - Liquid scintillation counting method (ISO 13162:2011)
Water quality - Determination of carbon 14 activity - Liquid scintillation counting method (ISO 13162:2011)
ISO 13162:2011 specifies the conditions for the determination of 14C activity concentration in samples of environmental water or of 14C-containing water using liquid scintillation counting.
The method is applicable to the analysis of any organic molecule soluble in water that is well mixed with the scintillation cocktail. It does not apply to micelles or "large" particles (lipids, fulvic acid, humic acid, etc.) that are inadequately mixed with the scintillation cocktail and the water. Some beta energy is lost without any excitation of the scintillation cocktail and the results are underestimated. The method is not applicable to the analysis of organically bound 14C, whose determination requires additional chemical processing (such as chemical oxidation, combustion).
It is possible to determine 14C activity concentrations below 106 Bq l-1 without any sample dilution.
Wasserbeschaffenheit - Bestimmung der Aktivität von Kohlenstoff-14 - Verfahren mit dem Flüssigszintillationszähler (ISO 13162:2011)
Qualité de l'eau - Détermination de l'activité volumique du carbone 14 - Méthode par comptage des scintillations en milieu liquide (ISO 13162:2011)
Kakovost vode - Določanje aktivnosti ogljika C-14 - Metoda štetja s tekočinskim scintilatorjem (ISO 13162:2011)
Ta mednarodni standard opisuje pogoje za določevanje koncentracije aktivnosti ogljika C-14 v vzorcih okoljske vode ali vode, ki vsebuje ogljik C-14, s štetjem s tekočinskim scintilatorjem. Metoda se uporablja za analizo katere koli organske molekule, topne v vodi, ki je dobro zmešana s scintilacijskim koktajlom. Ne uporablja se za micele ali »velike« delce (lipide, fulvično kislino, humično kislino itd.), ki so neustrezno zmešani s scintilacijskim koktajlom in vodo. Nekaj beta energije se izgubi brez vzbujanja scintilacijskega koktajla, pri čemer so rezultati podcenjeni. Metoda se ne uporablja za analizo organsko vezanega ogljika C-14, ki za določevanje zahteva dodatno kemično predelavo (na primer kemično oksidacijo, zgorevanje). Koncentracije aktivnosti ogljika C-14 pod 10(nad)6 Bq/l je mogoče določiti brez redčenja vzorca.
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Standards Content (Sample)
SLOVENSKI STANDARD
01-oktober-2015
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Water quality - Determination of carbon 14 activity -- Liquid scintillation counting method
(ISO 13162:2011)
Wasserbeschaffenheit - Bestimmung der Aktivität von Kohlenstoff-14 - Verfahren mit
dem Flüssigszintillationszähler (ISO 13162:2011)
Qualité de l'eau - Détermination de l'activité volumique du carbone 14 - Méthode par
comptage des scintillations en milieu liquide (ISO 13162:2011)
Ta slovenski standard je istoveten z: EN ISO 13162:2015
ICS:
13.060.60 Preiskava fizikalnih lastnosti Examination of physical
vode properties of water
17.240 Merjenje sevanja Radiation measurements
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 13162
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2015
ICS 17.240; 13.060.60
English Version
Water quality - Determination of carbon 14 activity - Liquid
scintillation counting method (ISO 13162:2011)
Qualité de l'eau - Détermination de l'activité volumique du Wasserbeschaffenheit - Bestimmung der Aktivität von
carbone 14 - Méthode par comptage des scintillations en Kohlenstoff-14 - Verfahren mit dem
milieu liquide (ISO 13162:2011) Flüssigszintillationszähler (ISO 13162:2011)
This European Standard was approved by CEN on 30 July 2015.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13162:2015 E
worldwide for CEN national Members.
Contents
Page
European foreword .3
European foreword
The text of ISO 13162:2011 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 13162:2015 by
Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2016, and conflicting national standards shall be withdrawn
at the latest by February 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 13162:2011 has been approved by CEN as EN ISO 13162:2015 without any modification.
INTERNATIONAL ISO
STANDARD 13162
First edition
2011-11-01
Water quality — Determination of
carbon 14 activity — Liquid scintillation
counting method
Qualité de l’eau — Détermination de l’activité volumique du
carbone 14 — Méthode par comptage des scintillations en milieu liquide
Reference number
ISO 13162:2011(E)
©
ISO 2011
ISO 13162:2011(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
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Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2011 – All rights reserved
ISO 13162:2011(E)
Contents Page
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Symbols, definitions, units, and abbreviations . 2
4 Principle . 2
5 Reagents and equipment . 3
5.1 Reagents . 3
5.2 Equipment . 4
6 Sampling and samples . 5
6.1 Sampling . 5
6.2 Sample storage . 5
7 Procedure . 5
7.1 Sample preparation . 5
7.2 Preparation of the sources to be measured . 5
7.3 Counting procedure . 6
7.4 Calibration and verification . 6
7.5 Measurement conditions . 6
8 Expression of results . 7
8.1 General . 7
8.2 Calculation of activity concentration . 7
8.3 Decision threshold . 8
8.4 Detection limit . 8
8.5 Confidence limits . 9
8.6 Calculations using the activity per mass . 9
9 Test report . 9
Annex A (informative) Numerical applications . 11
Annex B (informative) Internal standard method .13
Annex C (informative) Extraction of total carbon: precipitate counting .15
Annex D (informative) Extraction of total carbon: absorption counting .18
Bibliography .21
ISO 13162:2011(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13162 was prepared by Technical Committee ISO/TC 147, Water quality.
iv © ISO 2011 – All rights reserved
ISO 13162:2011(E)
Introduction
The carbon 14 ( C) present in the environment is of natural origin and man made. As a result of atmospheric
nuclear weapon testing, emissions from nuclear engineering installations, and the application and processing
of isotopes, relatively large amounts of C have been released into the environment. Due to the substantial
14 14
proportion of C in the human internal dose contribution, monitoring of C activity concentrations in the
environment is necessary in order to follow its circulation in the hydrosphere and biosphere. C is the second
radionuclide (�3 500 Bq) to contribute to the human body natural radioactivity, behind K (�6 000 Bq).
INTERNATIONAL STANDARD ISO 13162:2011(E)
Water quality — Determination of carbon 14 activity — Liquid
scintillation counting method
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This standard does not purport to address all of the safety problems, if any, associated with
its use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this International Standard
be carried out by suitably trained staff.
1 Scope
This International Standard specifies the conditions for the determination of C activity concentration in
samples of environmental water or of C-containing water using liquid scintillation counting.
The method is applicable to the analysis of any organic molecule soluble in water that is well mixed with the
scintillation cocktail. It does not apply to micelles or “large” particles (lipids, fulvic acid, humic acid, etc.) that are
inadequately mixed with the scintillation cocktail and the water. Some beta energy is lost without any excitation
of the scintillation cocktail and th
...
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