EN ISO 14183:2008

Animal feeding stuffs - Determination of monensin, narasin and salinomycin contents - Liquid chromatographic method using post-column derivatization (ISO 14183:2005)

ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method.
The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.

Futtermittel - Bestimmung der Gehalte an Monensin, Narasin und Salinomycin - Flüssigkeitschromatographisches Verfahren mittels Nachsäulenderivatisierung (ISO 14183:2005)

Dieses Dokument legt ein Verfahren der Hochleistungsflüssigchromatographie (HPLC) zur Bestimmung der Monensin , Narasin  und Salinomycin Gehalte in Futtermitteln, Futtermittel Zusatzstoffen (trocken und flüssig) und mineralischen Futtermittel Vormischungen fest. Das Verfahren ist nicht anwendbar auf Gemische, die chemische Zusatzstoffe (pharmazeutische Produkte) enthalten. Lasalocid und Semduramicin können nach diesem Verfahren nicht bestimmt werden.
Die Bestimmungsgrenze liegt für Monensin etwa bei 1 mg/kg, für Salinomycin etwa bei 2 mg/kg und für Narasin etwa bei 2 mg/kg. Eine niedrigere Bestimmungsgrenze kann angewendet werden, ist dann aber vom Anwender zu validieren.

Aliments des animaux - Détermination des teneurs en monensine, narasine et salinomycine - Méthode par chromatographie liquide utilisant la dérivatisation post-colonne (ISO 14183:2005)

L'ISO 14183:2005 spécifie une méthode de détermination des teneurs en monensine, en narasine et en salinomycine dans les aliments pour animaux, les compléments (secs et liquides) et les prémélanges de minéraux, par chromatographie liquide à haute performance (CLHP). La méthode ne s'applique pas aux prémélanges médicamenteux (produits pharmaceutiques) et ne permet pas de déterminer le lasalocide et la semduramicine.
La limite de quantification est respectivement d'environ 1 mg/kg, 2 mg/kg et 2 mg/kg pour la monensine, la salinomycine et la narasine. Une limite de quantification inférieure peut être atteinte mais cela est sujet à validation par l'utilisateur.

Krma - Določevanje monezina, narazina in salinomicina - Metoda tekočinske kromatografije s postkolonsko derivatizacijo (ISO 14183:2005)

Ta evropski standard določa metodo tekočinske kromatografije visoke ločljivosti (HPLC) za določevanje vrednosti monezina, narazina in salinomicina v krmi, dodatkih (suhih in tekočih) ter mineralnih mešanicah. Ta metoda se ne uporablja za predmešanice za zdravilno krmno mešanico (farmacevtski izdelki). Vrednosti lasalocida in semduramicina ni mogoče določiti s to metodo. Za monezin, salinomicin in narazin so meje kvantifikacije približno 1 mg/kg, 2 mg/kg in 2 mg/kg. Nižja meja kvantifikacije je dosegljiva, vendar jo mora validirati uporabnik.

General Information

• Status: Published
• Publication Date: 11-Nov-2008
• Withdrawal Date: 30-May-2009
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
• Drafting Committee: CEN/TC 327/WG 3 - Feed additives and drugs
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 12-Nov-2008
• Due Date: 15-Oct-2008
• Completion Date: 12-Nov-2008
• Directive: 70/373/EC - Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs
• Mandate: M/382 - Mandate for standardisation addressed to CEN in the field of Methods of analysis for animal Feedingstuffs
• Ref Project: SIST EN ISO 14183:2009 - Animal feeding stuffs - Determination of monensin, narasin and salinomycin contents - Liquid chromatographic method using post-column derivatization (ISO 14183:2005)

Relations

• Corrected By: EN ISO 14183:2008/AC:2012 - Animal feeding stuffs - Determination of monensin, narasin and salinomycin contents - Liquid chromatographic method using post-column derivatization - Technical Corrigendum 1 (ISO 14183:2005/Cor 1:2010)
• Effective Date: 27-Mar-2010

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 14183:2009
01-april-2009
.UPD'RORþHYDQMHPRQH]LQDQDUD]LQDLQVDOLQRPLFLQD0HWRGDWHNRþLQVNH
NURPDWRJUDILMHVSRVWNRORQVNRGHULYDWL]DFLMR,62
Animal feeding stuffs - Determination of monensin, narasin and salinomycin contents -
Liquid chromatographic method using post-column derivatization (ISO 14183:2005)
Futtermittel - Bestimmung der Gehalte an Monensin, Narasin und Salinomycin -
Flüssigkeitschromatographisches Verfahren mittels Nachsäulenderivatisierung (ISO
14183:2005)
Aliments des animaux - Détermination des teneurs en monensine, narasine et
salinomycine - Méthode par chromatographie liquide utilisant la dérivatisation post-
colonne (ISO 14183:2005)
Ta slovenski standard je istoveten z: EN ISO 14183:2008
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN ISO 14183:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 14183:2009

---------------------- Page: 2 ----------------------

SIST EN ISO 14183:2009
EUROPEAN STANDARD
EN ISO 14183
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2008
ICS 65.120

English Version
Animal feeding stuffs - Determination of monensin, narasin and
salinomycin contents - Liquid chromatographic method using
post-column derivatization (ISO 14183:2005)
Aliments des animaux - Détermination des teneurs en Futtermittel - Bestimmung der Gehalte an Monensin,
monensine, narasine et salinomycine - Méthode par Narasin und Salinomycin -
chromatographie liquide utilisant la dérivatisation post- Flüssigkeitschromatographisches Verfahren mittels
colonne (ISO 14183:2005) Nachsäulenderivatisierung (ISO 14183:2005)
This European Standard was approved by CEN on 25 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14183:2008: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 14183:2009
EN ISO 14183:2008 (E)
Contents Page
Foreword.3

2

---------------------- Page: 4 ----------------------

SIST EN ISO 14183:2009
EN ISO 14183:2008 (E)
Foreword
The text of ISO 14183:2005 has been prepared by Technical Committee ISO/TC 34 “Agricultural food
products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO
14183:2008 by Technical Committee CEN/TC 327 “Animal feeding stuffs - Methods of sampling and analysis”
the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the
latest by May 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 14183:2005 has been approved by CEN as a EN ISO 14183:2008 without any modification.

3

---------------------- Page: 5 ----------------------

SIST EN ISO 14183:200
...